Zeiss LSM800-6 Quick Start Manual

Zeiss LSM800-6 vertical-2 + Airyscan confocal microscope
I. System description:
Inverted (vertical) confocal microscope with Airyscan module
Motorized stage for multiple position imaging
Objectives:

Objective Plan-Apochromat 10x / NA 0.45 Air (WD=2.1mm)

Objective Plan-Apochromat 20x / NA 0.8 Air

Objective W-Plan-Apochromat 40x / NA 1.0 water dipping DIC VIS-IR

Objective C-Apochromat 63x / NA 1.2 W Korr

Laser lines: 405, 488, 561, 640 nm
Detectors:

2 PMT detectors with free choice of the spectral range. (PMT 1: tunable short pass and various
bandpass filters in front of the PMT, PMT 2: tunable long pass and various bandpass filters in
front of detector)

1 GaASP PMT for the Airyscan detector that can function as a normal detector in confocal mode
(detection range adjustable by a tunable short pass and a tunable long pass filter, no bandpass
filters)

An ESID detector for transmitted light.
Simultaneous detection of only two dyes possible, more than two have to be acquired sequentially.
II. Starting up:
1. Switch on the multiple socket (1). Provides power to microscope controllers
2. Switch on the system(2) and Components (3)
3. Switch on the computer (4)
4. Login: BIF-User (Useme!11)
5. Open ZEN Blue
your images)
6. If Pop-up appears about stage calibration, press "calibrate now"
Software and press "Start System" ("Image Processing" is for offline analysis of
2019-03-15
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