Zeiss LSM 880 Operating Manual page 195

LSM 880
Procedure to adjust Pinholes
• Mount a carrier with a suitable dye solution and orient the sample stage. Select the test sample.
• Select the beam path or the method with attached beam path you want to use.
• Set the laser power above 100 kHz, if possible.
• Open the Pinhole Adjust window (Fig. 270).
• Set the pinhole diameter as required
• Select the channel to which you want to adjust in a dual channel set up.
• Adjust both pinhole axes separately in the following order: x coarse, y coarse, x fine, y fine.
Both pinhole axes x and y must be adjusted. Otherwise the pinhole alignment will not be stored.
You might be able to increase count rate by adjusting the collimator in the Collimator panel for
invisible light. To find the optimum collimator settings use either the count rate or images. This
function should be changed with care only. The optimal collimator setting is pre-set in the factory.
For visible light there is no need for collimation.
In the Pinhole Alignment, the beam path is adjusted relative to the fixed pinhole. In a one channel
setting, only the selected channel is active and the chosen laser line used. In a two channel setup
both channels can be chosen for doing the pinhole alignment. If both laser lines are either from the
VIS or InVIS range than only one channel has to be adjusted, since the same beam path is used.
However, if one laser is from the VIS range and the other from InVIS, than you have to adjust both
channels. If only one laser line is activated with two channels selected, for example when using a
50/50 beam splitter, than this laser line is used either for channel 1 or channel 2.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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ZEISS
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