End - Zeiss LSM 880 Operating Manual

LSM 880
5.2.19.4

End

Expand the End field by clicking the
triangle (Fig. 230).
In the Mode drop down menu (Fig. 231)
you can select the way the acquisition is
triggered. You have the choice between
Manual, Trigger and Time.
If Manual is selected the acquisition of a
time series ends using the Stop button
or after the defined number of cycles.
If Trigger is selected the Trigger In drop
down menu becomes available (Fig. 232)
from which up to 4 triggers can be
selected. The system will interrupt the
acquisition once the trigger in signal is
received.
If Time is selected the hh, min and sec
input boxes become available (Fig. 233),
in which the hours, minutes and seconds
can be edited. The system interrupts the
acquisition after the defined computer
time has been elapsed.
It is possible to send a Trigger Out signal
with the end of the times series. The
Trigger Out drop down menu allows
defining up to 4 trigger out signals.
If the entered number of cycles is
processed without a set trigger
impulse been given to end the
procedure, the
finished.
If a trigger signal to end the scan arrives before the cycles have been processed, the Time Series will
only be interrupted. Waiting for Trigger will be displayed in the status line. The Time Series can
now be continued via a new trigger signal or ended via Stop.
A Time Series is finished when the end Time is reached.
If the entered number of Cycles has been processed, the Time Series is finished before the end
Time. If the number of cycles has not yet been processed until the end Time, the Time Series is also
finished. The end time for the Time Series can be changed online. Bleach times will be added to the
total time for image acquisition.
If a time series is interrupted before its programmed end, the programmed number of images will
be taken over in the database. However, only those images are stored which were created before
interruption of the time series. This is due to the fact that the original image parameters are to be
taken over via the Reuse function.
It is possible to stop and restart a time series via TriggerIN and have the data concatenated in one
file by alternating between the Start and End function. For example starting the time series with
TriggerIN 1 and stopping it via TriggerIN 2. Alternating between the two trigger inputs in essence
pauses the time series for a desired time.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
Time Series is
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Fig. 230 End field
Fig. 231 End Mode drop down menu
Fig. 232 End Mode drop down menu – Trigger
selected
Fig. 233 End Mode drop down menu – Time selected
ZEISS
161