Controls For Axio Examiner - Zeiss LSM 880 Operating Manual

LSM 880
(2) Microscope settings on Axio Imager for reflected-light observation (Epi-fluorescence)
• Turn on the HBO 100 W or X-Cite power supply with switch (Fig. 61/8)
• Click on the RL reflected light button. The shutter opens.
To avoid excessive bleaching of biological samples, expose the specimen to the minimum
possible irradiation, i.e. keep the irradiation time as short as possible. For this, use the slider in
the Ocular tool to attenuate the illumination of the HBO / X-Cite.
• By clicking on the reflector turret button, select the reflector module (filter sets) to suit the type of
fluorescence excitation. Proceed as follows:
• Click on the reflector turret button.
• Click on the desired reflector module.
− The reflector turret moves the selected reflector module into the beam path.
The FITC filter set consists of an excitation filter for the 450 - 490 nm spectral range, an FT color
splitter for 510 nm and an LP long pass filter, which passes emission light wavelengths greater
than 510 nm (FSET 09 = FITC, FSET 15 = Rhodamine, FSET 01 = DAPI).
Other filter sets:
DAPI:
Rhodamine: BP 546 FSET15
The filter sets described in this section are examples; other sets are available on request.
The aperture setting on the condenser of the Axio Imager.Z2 is performed in fixed steps.
5.1.3

Controls for Axio Examiner

For setting the Axio Examiner, proceed in the same way as with Axio Imager.Z2 and Axio Observer.Z1.
Since the objective turret of the Axio Examiner is not motorized the setting of the desired objective has to
be done manually.
The objective in use must be indicated in the Acquisition Mode tool.
10/2014 V_01
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
BP 365 FSET01
FT 395
LP 397
FT 580
LP 590
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ZEISS
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