Multidimensional Acquisition - Bleaching - Zeiss LSM 880 Operating Manual

ZEISS
Fig. 202 Bleaching tool
The repeated bleaching also allows bleaching the sample to an intensity value calculated as % of the
initial intensity within the region to bleach. To achieve this indicate the percentage value in the box next
to: Stop when Intensity drops to.
The number of Iterations indicates the total amount of scans which are performed for bleaching the
selected region (see also section during each bleach process.
A Different scan Speed for the bleaching process can be set independently from the scan speed which
is used for imaging. A lower speed results in a longer pixel dwell time, which increases the efficiency of
bleaching.
Different Z Position: If this option is active you can set the current stage position as the one in which
the bleaching will be done by clicking Mark Z. This function is only available using the Line or Frame
scanning mode.
When the imaging is performed in Spot Mode (chosen as acquisition mode in the Acquisition Mode
tool), a Different XY Spot Bleach Position can be selected. Click Spot Select in the Acquisition
Mode tool. A new image appears with a green crosshair. When clicking and dragging the crosshair a
red crosshair becomes visible. The red crosshair marks the spot that is imaged (This is also indicated in the
Scan Area of the Acquisition Mode tool. The green crosshair marks the spot that is bleached. Move the
center of the crosshairs to the desired positions.
Use one out of four triggers with Trigger In to trigger the bleaching process. In addition, one out of four
triggers can be used as Trigger Out signal to control an external device. (For a detailed description on
the trigger use and interface see printed manual CHAPTER 4: ANNEX).
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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5.2.16 Multidimensional
Bleaching
When the Bleaching tool tab is expanded the
Bleaching tool becomes available (Fig. 202).
The Bleaching tool is only available in LSM
Imaging mode.
The use of this function permits setting the bleach
parameters using the LSM 710 and LSM 780
systems for bleaching in spot, line or frame mode.
The Bleach Settings can be saved under a name
and reloaded for later use. Existing settings can
also be deleted from the list.
Bleaching works in
acquisition of a series of images over time. During
the sequential imaging of frames, lines or a spot,
bleaching of the specimen can be performed
according to the bleach settings defined in the
Bleaching tool.
Start Bleaching after # scans expects a number,
which indicates the amount of scanned frames,
lines, or spots after which then the bleaching of
the sample happens.
Repeat Bleach after # scans expects a number,
which indicates the amount of scanned frames,
lines, or spots after which then the bleaching of
the sample is repeated.
LSM 880
Acquisition
conjunction with the
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