Zeiss LSM 880 Operating Manual page 281

LSM 880
(2) Correlation G(t)
The diagram shows the correlation functions for each activated channel. The zoom function can be used
to display certain diagram areas of interest in an enlarged form. Use the left mouse button to click on the
margin of the area of interest in the diagram, keep the mouse button pressed and draw a rectangular
above the area of interest in any required direction. On release of the mouse button, the selected area is
displayed in an enlarged form in the diagram. The scaling of axes is matched automatically.
By clicking the right mouse button you can choose
between different options in the Correlation
context menu (Fig. 367).
• Select the line Reset Diagram Zoom with the
left mouse button to reset the zoom value.
The data (measuring values) of the diagram can be
copied to the clipboard or stored directly as an
ASCII file via the context menu.
• Select the line Copy data to clipboard with a
click of the mouse if you want to insert the data
directly into other WINDOWS programs via the
clipboard. The Paste function permits direct
insertion of these data into the required
program.
• Select the line Write text to file to store the data in an external ASCII file (.txt). Normalized values
will also be exported in this way.
Note that the Export button in the Display Correlation window will only export the original
values.
The diagram display can be manipulated with the context menu as follows:
• Selection of Abscissa logarithmic will scale the x-axis logarithmically
• Selection of Ordinate logarithmic will scale the y-axis logarithmically.
• The line width allows you to select the thickness of the graph. Six settings are possible.
• Selection of Normalize will normalize the curves to 2 (Fig. 368):
If Normalize is selected the G(0) value corresponding to the number of diffusing particles will be
normalized to 2, not the total correlation to which also other processes like triplet state can
contribute. All other values (subtracted by 1) will than be multiplied by the correction factor defined
by the ratio of 1 to [G(0)-1] and increased by 1, hence G (t)
Deactivating Normalize will display the normal correlation curve.
Normalization only works for a curve, for which a fit has been conducted in FCS Fit and hence a
G(0) is available.
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CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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Fig. 367 Correlation context menu
=[1/ (G(0)-1) * (G(t)-1)]+1.
normalized
ZEISS
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