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Imaging and Flow Cytometry Core CPOS
J. Airyscan imaging set up
1.
Follow air to oil immersion lens change
procedure in training. Switch to 63x objective.
2.
Select pre-set with prefix '0IFCore_Airyscan...'
or
3.
Click 'Smart setup'
4.
select fluorescent dyes and then click '+' button.
5.
Select 'Airyscan' mode.
6.
click 'OK'
7.
Confirm the following settings in 'Acquisition
Mode' window:
8.
'Frame Size' = 256 x 256 px.
intensity adjustment preventing pre-mature
sample bleaching)
9.
'bit' = Either 8 or 16 but follow carefully in step
[J19] when adjusting intensity parameter.
10. 'Scan Speed' = maximum
11. 'Zoom' > 1.3x
12. Start by only checking √ ONE channel in the
'channels' window.
13. Adjust detection wavelength range to suit your
particular fluorescent dye.
14. Turn on 'Continuous' scan mode
15.
! Important!
Go to 'Airyscan' tab in centre
preview area.
16. Click on 'Display' tab under centre preview area.
17. Turn on 'Auto' checkbox in 'Display' tab and
select 'Min/Max' option for real time intensity
display.
18. Adjust 'Master Gain' between 500V to 850V.
ver. 2.1 (Updated: 11/13/2020)
LSM 800 SOP
(Please refer to steps F26 to F32 for graphical
guidance)
(This is to facilitate
J2
J3
[Refer to Step F9 for graphical guide.]
J15
J17
J-15
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