Zeiss LSM 510 DuoScan Confocal Microscope Manuals

Manuals and User Guides for Zeiss LSM 510 DuoScan Confocal Microscope. We have 1 Zeiss LSM 510 DuoScan Confocal Microscope manual available for free PDF download: Manual

Zeiss LSM 510 DuoScan Manual

Zeiss LSM 510 DuoScan Manual (606 pages)

Brand: Zeiss | Category: Microscope | Size: 33.4 MB

Table of Contents

Chapter 1 Notes on Device Safety

5
Chapter 2 Lsm 510 and Lsm 510 Meta - Setup Requirements

31
Chapter 3 Introduction to Laser Scanning Microscopy

53
Chapter 4 Operation in Expert Mode

63
- Operation in Expert Mode
  71
- Purpose
  71
- Software
  71
- Windows and Window Elements
  72
- Backup
  73
- Convention for the Text in this Manual
  73
- Software Operation
  73
- Switching on the System
  74
- Switching on the Enterprise UV Laser
  75
- Starting the LSM 5 Program
  76
- Main Menu
  78
- File Menu
  80
- Create New Image Database
  81
- Image Database Window
  82
- Open Existing Image Database
  82
- Form Display Mode
  84
- Gallery Display Mode
  86
- Load an Image into the Image Display Window
  87
- Copy / Paste Image(S) to the Clipboard
  88
- Load Image with Reduced Resolution (Subset)
  88
- Update Image Database Display (Refresh)
  88
- Display Images with Certain Features (Filter)
  89
- Delete Function
  91
- On Filter Function
  91
- Save an Image to the Image Database
  92
- Import of Images
  95
- Export of Images
  96
- Multi Print
  97
- Overlay Toolbar
  97
- Exit the Expert Mode
  99
- Printing Images
  99
- Acquire Menu
  100
- Function Description
  101
- Laser Control
  101
- Opening / Closing the Laser Control Window
  101
- Settings
  102
- Microscope Control
  103
- Open the Microscope Control Window
  103
- Microscope Control Window for Axio Imager.z1
  104
- Microscope Control Window for Axiovert 200 M
  109
- Microscope Control for Axioskop 2 FS
  113
- Working in LSM Mode
  113
- Configuration Control
  114
- Open / Close the Configuration Control Window
  115
- Spectra Button
  115
- Config Button
  116
- Laserline Button
  116
- Settings for Single Track in the Channel Mode
  118
- Settings for Multi Track in the Channel Mode
  123
- Ratio Settings Panel
  127
- Settings in the Lambda Mode
  128
- Settings in the Online Fingerprinting Mode
  130
- Non Descanned Panel
  131
- Camera Detection Panel
  132
- Scan Control
  133
- Open / Close the Scan Control Window
  134
- Frame
  136
- Line
  154
- Spot
  159
- Camera Control
  160
- Edit ROI (Region of Interest)
  162
- Function Description
  162
- Open / Close the Edit ROI Window
  162
- Open / Close the Time Series Control Window
  166
- Time Series
  166
- Start Series Panel
  167
- Stop Series Panel
  169
- Cycle Delay / Time Interval Panels
  170
- Marker Panel
  172
- Time Series of a Frame
  174
- Time Series of a Frame over Z Stack
  176
- Time Series of a Frame over a Z Stack over Lambda
  177
- Time Series with Mean ROI
  178
- Edit Bleach
  180
- Open / Close the Edit Bleach Window
  180
- Settings Panel
  181
- Bleach Parameter Panel
  182
- Excitation of Bleach Track Panel
  183
- Stage
  184
- Start / End a Bleaching Procedure
  184
- Focus Position Panel
  185
- Open / Close the Stage and Focus Control Window
  185
- Stage Position Panel
  186
- Tile Scan Panel
  188
- VIS, TV and LSM Buttons
  190
- Add
  191
- Open / Close the Add Window
  191
- Process Menu
  191
- Destination Panel
  192
- Source Panel
  192
- Add Panel
  193
- Preview Panel
  193
- Open / Close the Subtract Window
  194
- Performance of the Subtract Function
  194
- Subtract
  194
- Multiply
  195
- Open / Close the Multiply Window
  195
- Performance of the Multiply Function
  195
- Open / Close the Ratio Window
  196
- Performance of the Ratio Function
  196
- Ratio
  196
- Copy (Channel)
  197
- Formula Panel
  197
- Open / Close the Copy Window
  197
- Performance of the Copy Function
  197
- Duplication (Image)
  198
- Filter
  198
- Image Panel
  198
- Open / Close the Filter Window
  198
- Filter List and Edit Panel
  199
- Contrast
  202
- Preview Panel
  202
- Image Panel
  203
- Interpolate
  203
- Open / Close the Interpolate Brightness and Contrast Window
  203
- Interpolation Panel
  204
- Channel Shift
  205
- Open / Close the Channel Shift Window
  205
- Preview Panel
  205
- Image Panel
  206
- Shift Panel
  206
- Preview Panel
  207
- Definition of Channels Panel
  208
- Open / Close the Unmix Window
  208
- Source Panel
  208
- Unmix
  208
- Function Description
  211
- Ion Concentration
  211
- Open / Close the Ion Concentration Window
  211
- Single Wavelength Dyes - Offline Calibration
  212
- Ratiometric Dyes
  213
- Ratiometric Dyes - Online Ratio
  213
- Ratiometric Dyes - Calibration
  214
- Kinetic
  215
- Open / Close the Kinetic Analysis Window
  215
- Options for Calibration Image Selection (Equation- or Titration Calibration)
  215
- Ratiometric Dyes - Equation Calibration (Grynkiewicz)
  215
- Function Description
  216
- Example: FRAP Performed in a Nucleus Expressing GFP Labeled Proteins
  217
- Copy Full Resolution
  220
- Copy Window Contents
  220
- Image Scaling
  220
- Paste Bitmap
  220
- Depthcod (Color Coded Depth Map)
  221
- Open / Close the Depth Coding Window
  221
- View Menu
  221
- Depth Coding Panel
  222
- Source Panel
  222
- Open / Close the Projection Window
  224
- Preview Panel
  224
- Projection
  224
- Source Panel
  224
- Projection Panel
  225
- Preview Panel
  226
- Open / Close the Stereo Images Window
  228
- Source Panel
  228
- Stereo
  228
- Stereo Images Panel
  229
- Preview Panel
  231
- Deconvolution (DCV)
  232
- Open / Close the 3D Deconvolution Window
  233
- Deconvolution Panel
  234
- Source Panel
  234
- Macro Language
  236
- Macro Menu
  236
- Edit Macro Function
  237
- Macro Control
  237
- Open / Close the Macro Control Window
  237
- Assign Macro to Button Function
  240
- Editing and Debugging of Macros
  241
- Overview of Available Macros
  242
- Sample Macros
  244
- Scanfieldtransform Macro
  244
- Distance Macro - Distance.lvb
  246
- Bleach Macro - Bleach.lvb
  247
- Profile Macro - Profile.lvb
  247
- Excitation Fingerprinting
  248
- Concatenate Images
  252
- Multiple Time Series Macro
  254
- Stitchart Plus Macro
  255
- Open / Close the Visual Macro Editor Window
  265
- Visual Macro Editor
  265
- Function Description
  266
- Editing a Macro
  271
- Export RM Function
  274
- Options Menu
  274
- Load RM Function
  275
- Dye DB Function
  277
- Settings Function
  278
- Spectra DB Function
  278
- Autosave
  279
- Open / Close the Settings for User
  279
- Database General
  280
- Database Gallery Viewer
  281
- Database Table Viewer
  281
- Image Status Display
  282
- Import / Export
  282
- Scan Information
  282
- Print Status Display
  283
- Recording / Reuse
  283
- Time Series
  283
- Scan Mean of Rois
  284
- Hardware
  285
- Program Start
  285
- Temporary Files
  285
- Image Display
  286
- Save
  287
- Electrical Calibration
  288
- Maintain Menu
  288
- Scanner
  288
- Optical Calibration with Speed 11-13 (Bidirectional)
  289
- Important Notes and Hints
  294
- Scanner Calibration in LSM 5 Software
  295
- Objective
  296
- Objective Change
  296
- Focus Speed Change
  298
- Parfocality Correction
  298
- Open / Close the Pinhole & Collimator Control Window
  299
- Pinhole Adjustment
  299
- Pinhole Panel
  300
- Collimator Panel
  301
- Pinhole and Collimator Adjustment
  302
- Set Find
  304
- Spline
  304
- Camera
  305
- Hwadmin
  305
- Test Grid
  305
- Close All Image Display Windows
  306
- Full Screen
  306
- Window Menu
  306
- Scan and System Information
  307
- About
  308
- FRAP Guide
  308
- Help
  308
- Help Menu
  308
- Display and Analysis of Images
  309
- Structure of the Image Display Window
  309
- Display Modes
  310
- Select - Chan
  311
- Assigning Another Color to a Channel
  312
- Defining a New Color
  312
- Switching a Channel of a Multi Channel Image off or on
  312
- Switching to Monochrome Image Display
  312
- Select - Zoom
  313
- Select - Slice
  314
- Functional Description
  315
- Select - Overlay
  315
- Buttons in the Overlay Toolbar
  316
- Select - Contr
  319
- Polyline
  320
- Ramp
  320
- Gamma
  321
- Spline
  321
- Select - Palette
  322
- Editing and Storing a Palette
  323
- Delete a Palette
  324
- Import a Palette
  324
- Select - Anim
  324
- Select - Reuse
  326
- Select - Crop
  327
- Select - Copy
  328
- Select - Save
  328
- Display - Xy
  329
- Select - Save as
  329
- Display - Split Xy
  330
- Display - Ortho
  331
- 4.13.17.1 Ortho - Select Function
  332
- 4.13.17.2 Ortho - Distance Function
  333
- 4.13.17.3 Ortho - 2D Deconvolution Function
  334
- Display - Cut
  335
- Display - Gallery
  336
- 4.13.20.1 Display - Histo - Overview
  338
- Display - Histo
  338
- 4.13.20.2 Area Function
  340
- 4.13.20.3 Colocalisation Function
  342
- 4.13.21.1 Function
  346
- Display - Profile
  346
- 4.13.21.2 Function Elements
  348
- 4.13.22.1 Direct Setting in the Image
  350
- 4.13.22.2 Setting Via Scrollbars
  350
- Display - 2.5 D
  350
- Display - 3D (Image Visart)
  352
- 4.13.23.1 Shadow Projection
  353
- Transparency Projection
  353
- 4.13.23.3 Surface Rendering
  357
- Appearance (Settings)
  358
- 4.13.23.5 Series
  359
- Display - Topography for LSM
  361
- 4.13.24.1 Channel Selection
  362
- 4.13.24.2 Generate Topography
  362
- 4.13.24.3 Topography Thresholds
  363
- 4.13.24.4 Processing by Filtering
  364
- 4.13.24.5 Display Modes
  367
- Context Menu of the 3D Display Mode
  370
- 4.13.24.7 Measurement Functions
  377
- 4.13.24.8 3D Measurement Functions
  387
- 4.13.24.10 Topo Reuse
  391
- 4.13.24.9 Export Data
  391
- Display - Prev
  392
- 4.13.25.1 Context Menu for Scan Information Text
  393
- 4.13.25.2 Context Menu for Topography Images
  393
- Arranging and Printing the Print Preview
  393
- Display - Info
  394
- 4.13.27.1 Display - Mean
  395
- Additional Display Mode in Time Series
  395
- 4.13.28.1 Display - Coded
  399
- 4.13.28.2 Display - Max
  399
- Additional Display Modes in Lambda Mode
  399
- 4.13.28.3 Display - Mean
  400
- Functionality for Emission Fingerprinting
  403
- 4.13.28.5 Automatic Component Extraction
  405
- Image Optimization
  407
- Requirements
  407
- Single Channel
  407
- Pinhole / Detector Gain / Ampl. Offset / Ampl. Gain
  411
- Scan Speed, Scan Average and Pixel Depth
  414
- Multiple-Channel
  415
- Requirements
  415
- Image Optimization
  416
- Extension of the LSM 510 or LSM 510 META with LSM Duoscan
  417
- Exiting the LSM Program
  418
- Shut-Down Procedure
  418
- Shut down the WINDOWS Operating System
  419
- Turning Power off
  419
- Software
  420
- Software and Hardware Options
  420
- Hardware
  422
- Courses on "How to Operate the System in an Optimized Way
  423
Chapter 5 Vba Programming for Lsm

425
- General Syntax
  426
- VBA Programming
  426
- Vba Programming for Lsm
  426
- Working with Files
  426
- Access to Windows API Function and External Dlls
  428
- Object Structure
  432
- Programming for LSM
  432
- Differences in Access to LSM Hardware with Lsm5Hardware Object / Dsrecording Object
  434
- Access to Hidden Interface
  437
- Access to Scanned Pictures
  438
- Backup Recording
  440
- Events
  441
Chapter 6 Routine Mode and Tools

443
- General
  444
- Routine Mode
  444
- Routine Mode and Tools
  444
- Starting the Routine Mode
  444
- Application of Examination Methods in the Routine Mode
  446
- Acquisition of a Frame
  447
- Acquisition of a Z Stack
  451
- Acquisition of a Time Series
  456
- Change Filters
  461
- Tools
  461
- Stand Select
  464
- LSM Image Browser
  465
- LSM Image Examiner
  466
Table of Contents

467
- For LSM
  468
- Overview and Explanations
  468
- The Image Sequence
  468
- Introduction
  469
- Memory Usage
  469
- The Image Properties
  469
- User Interface
  469
- Display Window
  470
- Main Window
  470
- Functions
  477
- Functions in the File Menu
  477
- Functions in the Edit Menu
  480
- Functions in the Process Menu
  483
- Functions in the View Menu
  509
- Functions in the Measurement Menu
  516
Chapter 8 Annex

527
Table of Contents

565

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