Attaching The New Tubing To The Transfer Line - Thermo Scientific TRACE 1600 Hardware Manual

Attaching the New Tubing to the Transfer Line

Your kit comes with a 0.6 m and 1.2 m deactivated segments of fused silica tubing having an
internal diameter of 0.17 mm. This tubing has fused ends. You must cut these ends off the
tubing and attach a SilFlow ferrule before connecting it. Use this tubing to connect the
microfluidics splitter to the transfer line.
Note Some of the cleaning procedures in this section require the use of methanol.
If methanol is unavailable or prohibited, substitute LCMS-grade or GC-grade ethanol
or isopropyl alcohol. Do not use denatured ethanol as it may contain impurities that
contaminate the GC-MS system.
Note Use the 60 cm tubing if you are using helium as a carrier gas. Use the 120 cm length
of tubing if you are using hydrogen as a carrier gas.
When connecting the column to the transfer line, you may use either the regular transfer line
nut or the spring loaded transfer line nut with the graphite Vespel® ferrule.

To connect the column using the regular transfer line nut
1. Lower the oven temperature and allow it to cool.
2. Confirm that the MS is vented and remove the current transfer line nut and ferrule.
3. Unwind about one turn of the column from the column outlet end.
Note Wear clean, lint- and powder-free gloves when you handle the column and
transfer line ferrule.
4. Wipe approximately 300 mm (12 in.) of the column with a tissue soaked in methanol.
5. Choose an appropriate ferrule for the outer diameter of your column.
Note If the maximum oven temperature in your method is ≥ 290 °C (554 °F),
Thermo Fisher Scientific recommends using a spring loaded transfer line nut with a
graphite Vespel® ferrule or a SilTite™ nut and ferrule. By cycling the oven at and above
this temperature, expansion and contraction of the graphite Vespel® material can
cause leaks in the transfer line.
6. Insert the column through the transfer line nut and ferrule, entering through the tapered
end of the ferrule. Wipe the column again with a tissue soaked in methanol.