Thermo Scientific TRACE 1600 Hardware Manual page 111

Thermo Scientific
Column conditioning consists of passing a carrier gas flow through the column heated at
a programmed temperatures as described in the column manufacturer's instructions.
In case the column does not have any column conditioning instructions, perform the
column conditioning by setting a final temperature up to 10 °C - 20 °C below its
recommended maximum temperature.
CAUTION When performing column conditioning, connect the column only to the
injector leaving the column outlet disconnected to avoid the possibility of contamination
of the detector.
Do not use hydrogen as the carrier for conditioning! It could vent into the oven and
present an explosion hazard.
ATTENTION Pendant le conditionnement de la colonne, branchez uniquement cette
dernière à l'injecteur. La sortie de colonne ne doit pas être branchée, afin d'éviter toute
contamination du détecteur.
N'utilisez pas l'hydrogène comme gaz vecteur pour le conditionnement ! Il risquerait de
s'échapper dans le four et de présenter un risque d'explosion.
a. Run the slow temperature program that is recommended by the manufacturer.
INSTRUMENT DAMAGE: Never exceed the column manufacturer's maximum operating
temperature.
9. Connect the column to the detector inside the GC.
a. Lower the oven temperature to 30 °C and allow it to cool.
WARNING-BURN HAZARD: The injector, detectors, oven, and transfer line may be hot.
Allow them to cool to room temperature before touching them.
b. Unwind the column enough to easily connect its ends to the injector and the
detector.
Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.
c. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column outlet about 2.5cm (1 in.) from
the end. Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
e. Insert the column through the proper detector retaining nut and ferrule (open end
up). Wipe the column again with a tissue soaked in methanol.
Tip Slide a notched septum on the column before the detector retaining nut to
make it easier to measure the proper distance between the bottom nut and end of
the column.
2
Performing Routine Maintenance
Replacing a Column
TRACE 1600/1610 Hardware Manual 79