Thermo Scientific TriPlus RSH User Manual
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Thermo Scientific TriPlus RSH User Manual

Robotic sample handling
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Thermo Scientific
TriPlus RSH
Robotic Sample Handling
User Guide
P/N 31709620 Ninth Edition December 2015

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Summary of Contents for Thermo Scientific TriPlus RSH

  • Page 1 Thermo Scientific TriPlus RSH Robotic Sample Handling User Guide P/N 31709620 Ninth Edition December 2015...
  • Page 2 © 2015 Thermo Fisher Scientific Inc. All rights reserved. TriPlus RSH, TRACE 1300, TRACE 1310, TRACE GC Ultra and FOCUS GC are trademarks of Thermo Fisher Scientific Inc., and its subsidiaries. Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy Tel: +39 02 95059303;...
  • Page 3 Reader’s Survey TriPlus RSH User Guide, PN 31709620, Ninth Edition Strongly Strongly Agree Neutral Disagree Agree Disagree The manual is well organized. The manual is clearly written. The manual contains all the information I need. The instructions are easy to follow.
  • Page 5 EN 61000-6-2:2005 | IEC 61000-6-2:2nd Edition| IEC 61000-6-3:2nd Edition am1 | EN 61000-6-3:2007 + A1:2011 • Conducted Emission, Subpart B. FCC part 15, §15.107(a) and §15.109(a) Laser Class 1 • The selected Class 1 Laser for the TriPlus RSH module Barcode Reader complies with the following regula- tions:...
  • Page 6 Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument.
  • Page 7 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 8 Conformità RAEE Questo prodotto è marcato con il seguente simbolo in conformità alla direttiva europea 2012/19/EU (RAEE) sui rifiuti di apparecchiature elettriche ed elettroniche: Thermo Fisher Scientific si è accordata con una o più società di riciclaggio in ciascun Stato Membro della Unione Europea (EU), e queste società...

  • Page 9: Table Of Contents

    Getting Familiar with Your TriPlus RSH ........1...
  • Page 10 TriPlus RSH for Headspace........
  • Page 11 How to Start the TriPlus RSH ........
  • Page 12 Setup Menu Item - Set Date and Time ......176 Setup Menu Item - Set TriPlus RSH Name ......177 Setup Menu Item - Setup Network .
  • Page 13 Set TriPlus RSH Name ........

  • Page 15: Preface

    This includes operating procedures, sample injection techniques, diagrams, and the description of the major components. This User Guide is intended for frequent or new TriPlus RSH users who are experienced at using automated systems to run existing analytical methods.

  • Page 16: About Your System

    Thermo Fisher Scientific office, or affiliate GC-GC/MS Customer Support. Related Documentation In addition to this guide, Thermo Scientific provides the following documents for the TriPlus RSH. • TriPlus RSH Safety Guide, PN 31709600 • TriPlus RSH Preinstallation Requirements Guide, PN 31709610 •...

  • Page 17: Safety Alerts And Important Information

    ELECTRICAL SHOCK HAZARD: Indicates that an electrical shock could or might occur. FIRE HAZARD: Indicates a risk of fire or flammability could or might occur. EXPLOSION HAZARD. Indicates an explosion hazard. This symbol indicates this risk could or might cause physical injury. Thermo Scientific TriPlus RSH User Guide xvii...
  • Page 18 TOXIC SUBSTANCES HAZARD: Indicates that exposure to a toxic substance could occur and that exposure could or might cause personal injury or death. LASER HAZARD. Indicates that exposure to a laser beam will, could, or might cause personal injury. xviii TriPlus RSH User Guide Thermo Scientific...

  • Page 19: Instrument Markings And Symbols

    Instrument Markings and Symbols Table 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of them are used on the TriPlus RSH. See the asterisk. Table 1. Instrument Marking and Symbols (Sheet 1 of 2) Symbol...

  • Page 20: Safety Information And Warnings

    Thermo Fisher Scientific representatives during installation, and repair of the TriPlus RSH, or parts of it (following the life cycle principle), as well as for the end user TriPlus RSH in the lab during the learning phase, and in routine work.

  • Page 21: Electrical Hazards

    Poor grounding represents a danger for the operator, and might seriously affect the performance of the instrument. Do not connect the TriPlus RSH to power lines that supply devices of a heavy duty nature, such as motors, refrigerators and other devices that can generate electrical disturbances.

  • Page 22: Laser Safety Information

    Never try to repair or replace any components of the instrument without the assistance of a Thermo Fisher Scientific representative. There are no operator-serviceable or replaceable parts inside the power supply(ies) or in the TriPlus RSH. If a power supply is not functioning, contact a Thermo Fisher Scientific representative.

  • Page 23: Other Hazards

    TriPlus RSH. Danger of crushing to fingers and hands. To avoid injury keep your hands away from moving parts during operation. Turn off the power to the TriPlus RSH if you must reach inside a mechanically powered system with moving parts.
  • Page 24 Depending on the physical, chemical or hazardous properties of the solvent, use the appropriate protective measures for handling. xxiv TriPlus RSH User Guide Thermo Scientific...

  • Page 25: Working With Toxic Or Other Harmful Compounds

    • Instructions for decontamination and safe disposal of the relevant parts must be provided. It is your responsibility to handle hazardous chemicals or biological compounds (including, but not limited to, bacterial or viral samples and the associated waste), safely and in accordance with international and local regulations. Thermo Scientific TriPlus RSH User Guide...

  • Page 26: Maintenance

    Safety Information and Warnings Maintenance Any external cleaning or maintenance must be performed with the TriPlus RSH turned off and the power cord disconnected. Avoid using solvents and spraying on electrical parts. For the removal of potentially dangerous substances (toxic, harmful, and so on) read the hazard indications and information reported in the MSDS (Material Safety Data Sheet) supplied by the manufacturer referring to the relevant CAS (Chemical Abstract Service) number.

  • Page 27: Chapter 1 Getting Familiar With Your Triplus Rsh

    Getting Familiar with Your TriPlus RSH This chapter provides informations to familiarize with the TriPlus RSH. Contents • Instrument Basics • Definition of Terms, Naming Conventions, and Start Screen Icons • The TriPlus RSH System • TriPlus RSH Configurations •...

  • Page 28: Instrument Basics

    • Barcode reader with dual scan module for barcode identification in any position. • Wide range of predefined cycles for advanced liquid handling. The TriPlus RSH can be set up and configured using either the Handheld Controller or the Virtual Handheld Controller (PC software).

  • Page 29: Definition Of Terms, Naming Conventions, And Start Screen Icons

    • TriPlus RSH — A generic term referring to the TriPlus RSH system. TriPlus RSH is more used in combination with the basic unit with X- and Y-axes, Head (Z-axis), and Power installed. • Module — A generic term used to refer to both active and passive modules.
  • Page 30 Data System (CDS). A Script is built by linking Activities together in a sequence. • Script — Created by building a sequence of TriPlus RSH operations, which are linked by a series of Activities. A Script can be a Method depending on the functionality built-into the script.
  • Page 31 Plates are deepwell plates, or microtiter plates. The well plates have a standardized footprint. The tray holder drawers are designed to accept well plates. Passive tray holders for the TriPlus RSH have three slots, each one designed with the footprint dimensions of a Well Plate.

  • Page 32: Naming Conventions

    Naming Conventions This section provides the standard naming convention for TriPlus RSH Trays and Tray Types. Following these conventions will allow the TriPlus RSH to be pre-configured for certain applications, will simplify software backups and application development, and will improve technical support and training.

  • Page 33: Start Screen Icons

    Getting Familiar with Your TriPlus RSH Definition of Terms, Naming Conventions, and Start Screen Icons Table 1. Example of Tray Types Naming Convention (Sheet 2 of 2) Tray Type Tray Description MTP96 Well Plate Type. Microtiter Plate MT96. 96 positions (8 x 12).
  • Page 34 Getting Familiar with Your TriPlus RSH Definition of Terms, Naming Conventions, and Start Screen Icons Table 2. Start Screen Icons (Sheet 2 of 3) Icon Description SPME Fiber Conditioning Station Barcode Reader Temperature Controller Drawer OC Injector (On-column Injector) Fast Wash Module...
  • Page 35 Getting Familiar with Your TriPlus RSH Definition of Terms, Naming Conventions, and Start Screen Icons Table 2. Start Screen Icons (Sheet 3 of 3) Icon Description Synchronization and Signals Input and Output Signals Input Signal Output Signal Chromatographic System. Specifies synchronization of Input and Output Signals, Cryo Trap, and Delay Time for a Fake Injection for an analytical system.

  • Page 36: The Triplus Rsh System

    Getting Familiar with Your TriPlus RSH The TriPlus RSH System The TriPlus RSH System The TriPlus RSH is an integrated sampling system based on three axes X,Y and Z, constituted by a basic body on which a series of components are installed. See Figure Figure 1.

  • Page 37: Modules

    Getting Familiar with Your TriPlus RSH The TriPlus RSH System – Longitudinal Crossrail (X-axis) Represents the X-axis of the system, and the structure bearing the sampling unit components. The longitudinal crossrail (X-axis) is available in the standard or extended version.

  • Page 38: Optional Components

    Temperature Controlled Drawer. User Interface The TriPlus RSH can be set up and configured using either the Handheld Controller or the Virtual Handheld Controller (PC software) The functions of the TriPlus can be controlled through a data processing system for PC with dedicated software.

  • Page 39: Triplus Rsh Configurations

    TriPlus RSH Configurations TriPlus RSH Configurations This section provides a brief overview of the various configurations of the TriPlus RSH as applicable to the GC technique. The TriPlus RSH can be installed on the gas chromatographs for sample introduction into up to: three injectors by using the standard module longitudinal crossrail or four injectors by using the extended longitudinal crossrail.

  • Page 40: Triplus Rsh For Headspace

    Multiple Headspace Extraction (MHE) – Constant DoublePro TriPlus RSH for SPME (Solid Phase Micro Extraction) This version features the following characteristics: • Injectors into which the sample may be introduced — S/SL and PTV • Washing Station — Two vials and four vials, Fast Wash Station •...

  • Page 41: Triplus Rsh For Itex (In-Tube Extraction)

    Getting Familiar with Your TriPlus RSH TriPlus RSH Configurations TriPlus RSH for ITEX (In-tube Extraction) This version features the following characteristics: • Injectors into which the sample may be introduced — S/SL, PTV, PKD, and PPKD • ITEX2 Tool • Washing Station — Two vials and four vials •...

  • Page 42: Crossrails

    Getting Familiar with Your TriPlus RSH Crossrails Crossrails The crossrails constitutes the primary structure up on which the sampling unit components are installed according to the configuration of the sampler. The two sliding crossrails, shown in Figure 2, constitute the X and Y axes of the sampler. They are connected to one another through a mechanism that manages the movements of the system by both a motorized truck and a tray located in every crossrail.

  • Page 43: Head

    Getting Familiar with Your TriPlus RSH Head • Lower section — The part that slides along the crossrail X. Head It represents the Z-axis of the system. It consists of a vertical structure fixed on the crossrail Y which guides the movements along the X and Y axes. See Figure Figure 3.

  • Page 44: Triplus Rsh Tools

    Injection Device Motors TriPlus RSH Tools The TriPlus RSH requires more information and intelligence on the level of tools than just the syringe identification numbers as used with common samplers. The capability to use a tool for one task, store the tool away and select a second tool for the next task in the cycle sequence necessitates knowledge of the geometry of the syringe and holder.
  • Page 45 The syringe consists of the holder to insert and keep the tool in position, the upper and lower needle guide, and the PCB board. The following attributes are generally stored with a TriPlus RSH Tool: • Tool ID (Identification Number) •...

  • Page 46: Tool Identification

    Getting Familiar with Your TriPlus RSH TriPlus RSH Tools Figure 6. LS Tool with Needle Length 57 mm and 85 mm 85 mm Needle Length LS Tool 57 mm Needle Length LS Tool Tool Identification The tools are labeled at the side. The code identifies the Tool required for your’s needs. See the...

  • Page 47: Ls Tool

    Getting Familiar with Your TriPlus RSH LS Tool The Tools are branded by the factory for the basic configuration, which means: • Tool Type as Liquid Syringe (LS), Headspace (HS), and SPME. • Tool internal diameter to accept Syringe Glass Barrel with defined outer diameter, for example: 6.6 or 7.7 mm.
  • Page 48 Getting Familiar with Your TriPlus RSH LS Tool The LS Tool is a single block, easy to use to install a syringe. See Figure Figure 8. LS Tool The syringe flange is supported by an O-ring (support bearing). See Figure Figure 9.
  • Page 49 Getting Familiar with Your TriPlus RSH LS Tool At the lower part of the block a second O-ring is installed which guides the syringe glass barrel and prevents direct metal contact. See Figure Figure 10. O-Ring Second O-ring The Magnet Adapter Ring is used to transport vials from a Tray to the Agitator.

  • Page 50: Syringes For Ls Tools

    Getting Familiar with Your TriPlus RSH LS Tool Syringes for LS Tools Table 4 lists the type of syringes suitable for the LS tool. Table 4. List of Syringes for LS Tools Syringes Type Volume 7 mm diameter syringe barrel with 57 mm 0.5 μL, 1.0 μL, 5.0 μL, 10 μL, 25 μL, 50 μL,...

  • Page 51: Hs Tool

    Getting Familiar with Your TriPlus RSH HS Tool Table 5. LS Tool - Injector Compatibility (Sheet 2 of 2) Injector PKD injector • PKD injector — 57 mm needle length LS tool Automated Bottom Sensing with a syringe needle length of 85 mm is not recommended.
  • Page 52 Getting Familiar with Your TriPlus RSH HS Tool Figure 13 shows the cut-out of the HS tool visualizing the Purge Gas Flow and the Sealing O-ring. Figure 13. HS Tool Cut-out The Magnet Adapter Ring is used to transport vials from a Tray to the Agitator, as an example.

  • Page 53: Syringes For Hs Tools

    SPME Fiber Holder. Currently, there are two different fiber lengths commercially available: 10 mm and 20 mm. Both types can be adapted with the SPME Tool. The TriPlus RSH System software allows setting the fiber extension length to the necessary distance.
  • Page 54 Getting Familiar with Your TriPlus RSH SPME 1 and SPME 2 Tools The holder is fixed in position by the SPME Tool adapter and the retaining nut. No O-rings are necessary to fix or protect the SPME holder. A dedicated magnet is required for the SPME Tool.

  • Page 55: Syringes For Spme Tools

    Getting Familiar with Your TriPlus RSH SPME 1 and SPME 2 Tools Syringes for SPME Tools SPME syringe fiber Tool includes a combination of four different fibers for starting-up. Definition of SPME Penetration Depth Up to RSH Firmware version 1.5.0, the definition of the parameter for SPME Penetration Depth has been defined without including the Fiber length.

  • Page 56: Itex-2 Tool

    Getting Familiar with Your TriPlus RSH ITEX-2 Tool ITEX-2 Tool The ITEX-2 tool consists of a dedicated gas-tight syringe with an adapter for a replaceable side-hole needle and a heated syringe cartridge. The side-hole needle includes an integrated trap (standard trap material is Tenax® TA) and a trap desorber heater. See Figure Figure 18.
  • Page 57 Getting Familiar with Your TriPlus RSH ITEX-2 Tool WARNING - BURN HAZARD The ITEX Tool can operate at a maximum temperature of 200 °C. Operating at this temperature, its outer surface will reach about 70 °C. Do not touch the outside of the ITEX Tool. Always let the ITEX Tool cool down before you touch it, or before you remove the Syringe.
  • Page 58 Getting Familiar with Your TriPlus RSH ITEX-2 Tool The purge gas adapter mounted on the ITEX-Tool couples with the adapter from the head. The coupling is sealed with an O-ring installed in the ITEX Tool Purge Gas Adapter. See Figure Figure 21.

  • Page 59: Control Interface

    Green status light, blinking: TriPlus RSH is working (moving). Execution mode. The Handheld Controller is partially blocked to specific actions. Green status light, steady on: TriPlus RSH is ready in Standby. Idle Mode. Yellow status light, blinking: RSH Firmware is booting.
  • Page 60 Update or Backup. (For the processes Copy Backup and Restore the Status light remains green). Blue status light, permanent: TriPlus RSH is in a safe state (power reduced to a minimum) and requires manually moving the Head to the Teach Point.

  • Page 61: Definitions Of Active And Passive Hardware Modules

    Getting Familiar with Your TriPlus RSH Definitions of Active and Passive Hardware Modules Definitions of Active and Passive Hardware Modules This section details the definitions of the hardware modules. Active Versus Passive Module An Active module is defined as a module that sends or receives specific commands and checks.

  • Page 62: Active Versus Composite Module

    A Composite module, such as the TriPlus RSH, is composed of several devices. Note This differentiation is not important for the routine use of the TriPlus RSH. You must understand this difference when a device must be replaced, for example a motor-Z as part of the TriPlus RSH Head.

  • Page 63: Modules Description And Specifications

    Getting Familiar with Your TriPlus RSH Modules Description and Specifications Modules Description and Specifications This section covers the description and specifications of the following modules: • “TR Station” page 38 • “ATC Station” page 39 • “Standard Wash Station” page 40 •...

  • Page 64: Tr Station

    Getting Familiar with Your TriPlus RSH TR Station TR Station This passive module contains a single slot for an injection tool. See Figure 25 Figure Figure 25. TR Station Figure 26. TR Station with Tool TriPlus RSH User Guide Thermo Scientific...

  • Page 65: Atc Station

    Getting Familiar with Your TriPlus RSH ATC Station ATC Station This active module containing three slots for tools allows you to change an injection tool automatically for specific needs. See Figure 27 Figure Figure 27. ATC Station Figure 28. ATC Station with Tools...

  • Page 66: Standard Wash Station

    Getting Familiar with Your TriPlus RSH Standard Wash Station Standard Wash Station The Standard Wash Station is shown in Figure Figure 29. Standard Wash Station with Drainage Adapter Contains five 10 mL vials. The configuration within the Standard Wash Station can be flexible.

  • Page 67: Large Volume Wash Station

    Getting Familiar with Your TriPlus RSH Large Volume Wash Station Large Volume Wash Station The Large Volume Wash Station is shown in Figure Figure 30. Large Volume Wash Station Contains two 100 mL vials and one vial for waste port for diverting waste to large reservoir.

  • Page 68: Fast Wash Station

    Getting Familiar with Your TriPlus RSH Fast Wash Station Fast Wash Station The Fast Wash Station is shown in Figure Figure 32. Fast Wash Station The Fast Wash Station is an active module. Communication and control of the station is provided through the BUS from the system.

  • Page 69: Agitator

    Getting Familiar with Your TriPlus RSH Agitator Table 9. Fast Wash Station - Dimensions and Mass (Sheet 2 of 2) Length Dimension in mm (in.) Solvent reservoir kit 1.30 (2.87) Including two 1000 mL bottles Mass Dimension in kg (lbs) Fast Wash Station 0.750 (1.65)
  • Page 70 Getting Familiar with Your TriPlus RSH Agitator WARNING -BURN HAZARD The Agitator can operate at a maximum temperature of 200 °C. Operating at this temperature, its outer surface will reach approximately 70 °C. When the Agitator is heated, do not touch the outside surface, do not reach into it; do not try to remove vials manually from it.

  • Page 71: Vortexer

    Getting Familiar with Your TriPlus RSH Vortexer Table 12. Agitator - Operating and Environmental Requirements Parameter Requirements Operating temperature range 40 to 200°C Maximum relative humidity 80%, non-condensing Vibration Negligible Static electricity Negligible Vortexer The Vortexer is an active module which used for intensive shaking (orbital interval shaking) at ambient temperature.
  • Page 72 It is mandatory using the mixer with the installed protective cover. During the mixing step it is important to keep the TriPlus RSH Head always above the vial to avoid an uplift of the vial caused by the spin.

  • Page 73: Mhe Station

    Getting Familiar with Your TriPlus RSH MHE Station Table 14. Operating and Environmental Requirements Parameter Requirements Operating temperature range Ambient, no control Maximum relative humidity 80%, non-condensing Vibration To be tested Static electricity Negligible MHE Station The MHE (Multiple Headspace Extraction) Station is an add-on passive module to extend the range of headspace applications.
  • Page 74 Getting Familiar with Your TriPlus RSH MHE Station CAUTION The MHE Venting Adapter contains a spring-loaded white insert to protect the needle. Use caution when handling the white insert; the needle can pierce skin. Note The MHE Adapter has a threaded gas vent outlet, allowing an optional OD 1/16 inch waste tube to be attached.

  • Page 75: Naming Convention For Mhe Station

    Getting Familiar with Your TriPlus RSH MHE Station Naming Convention for MHE Station MHE Adapter — The MHE Adapter, equipped with a built-in needle, is used as the tool to release the pressure from the vial. The MHE Adapter is also equipped with a vent outlet connector for connecting a vent line. In cases toxic gases are released from the vial, the venting line can be positioned into a neutralizing agent or to a fume hood to protect the operator and the environment.
  • Page 76 Getting Familiar with Your TriPlus RSH MHE Station The effect of the sample matrix is eliminated by extracting the entire amount of the analyte. Contrary to the situation that exists in single-headspace extraction, it is therefore possible to use calibration standards that are not prepared with the same matrix as the actual samples. For MHE to provide meaningful results, the only prerequisite is that equilibrium must exist with respect to the analytes in terms of the distribution between the phases in the headspace vial.
  • Page 77 Getting Familiar with Your TriPlus RSH MHE Station We will look at four examples situations of equilibrium between the condensed (solid or liquid) phase and the gas phase (the headspace). The examples will be shown in increasing complexity with respect to the equilibrium distribution and removal of the headspace between subsequent extractions.
  • Page 78 Getting Familiar with Your TriPlus RSH MHE Station A(ref ) — Area that would be measured if all of the analyte were present in the headspace (again corresponding to the measured signal from a similar gas standard). A(0) — Actual area measured from the first extraction with 75% of the analyte in the gas phase: A(0) = ¾...
  • Page 79 Getting Familiar with Your TriPlus RSH MHE Station However, A(0) = ½ * A(ref ) and thus: A(tot) = ½ * A(ref ) * 2/a, or A(tot) = A(ref )/a If α =1 (the entire headspace is exchanged between each extraction) then: A(tot) = A(ref ) If α...

  • Page 80: Mhe Cycle Step-By-Step

    Getting Familiar with Your TriPlus RSH MHE Station If the MHE quantification is based on gas standards that are subjected to the same MHE extraction sequences as the actual samples, there will be no overestimation. The amount calculated will be correct.
  • Page 81 Getting Familiar with Your TriPlus RSH MHE Station Figure 36. MHE Cycle Steps Thermo Scientific TriPlus RSH User Guide...

  • Page 82: Quantification From Mhe Measurements

    Getting Familiar with Your TriPlus RSH MHE Station Quantification from MHE Measurements The raw data from analytical samples deriving from MHE measurements must be treated differently from those deriving from a classic, static headspace experiment. In general, software packages for chromatographic data handling are not designed to cope in an automated manner with MHE raw data.
  • Page 83 Getting Familiar with Your TriPlus RSH MHE Station Procedure for Determining Acetone Content The steps for determining the acetone content are outlined in the following example: • A — Each of the six calibration standards is extracted five times for increasing the quality of the determination).
  • Page 84 Getting Familiar with Your TriPlus RSH MHE Station Figure 37. Sample Excel Setup for MHE Quantitation The following is an overview of each of the steps involved in completing and interpreting the data shown the Excel spreadsheet of Figure Step 1 The measured analyte area corresponding to each of the extraction steps is typed here.
  • Page 85 Getting Familiar with Your TriPlus RSH MHE Station Figure 38. Entering Measured Areas of Calibration Standard Analyte Step 2 The natural logarithm of the measured analyte areas are plotted against the extraction number. Usually, this curve will show good linearity. The validity of the MHE procedure can be judged from this curve: if equilibrium exists, the curve will be linear, and the MHE procedure will work.
  • Page 86 Getting Familiar with Your TriPlus RSH MHE Station Step 4 The total area is the limit value of the geometric sum. The two parameters needed for the calculation of the sum are directly determined by the linear regression. They are the slope and the intercept of the regression curve, respectively.

  • Page 87: Spme Conditioning Station

    Getting Familiar with Your TriPlus RSH SPME Conditioning Station The total area is determined from the MHE-plot of Figure Consequently, the acetone content can be determined as follows: 1. Calculate the total area using the Excel spreadsheet. In this example the total area is 101.1 2.
  • Page 88 Getting Familiar with Your TriPlus RSH SPME Conditioning Station Figure 45. Spare Fiber Inserted in front Position The fiber is manually placed into the hole, and brought to the exposed position. If this second position is used, open the needle valve for gas flow in the second channel (front).

  • Page 89: Barcode Reader

    Getting Familiar with Your TriPlus RSH Barcode Reader Table 19. SPME Conditioning Station - Operating and Environmental Requirements Parameter Requirements Operating temperature range 30 to 350 °C Maximum relative humidity 80%, non-condensing Vibration Negligible Static electricity Negligible Barcode Reader The Barcode Reader is an active module used to decode and read barcodes applied in horizontal bars on vials of 1 to 20 mL volume.

  • Page 90: Safety Information

    • Continue with sample handling but stamp the field Sample ID with an error comment BC not readable. • Stop the process of data handling, stop the entire Sample List. This approach of using two scanners confers the advantage that the vial with the barcode label does not require to be oriented directly towards the scanner.

  • Page 91: Supported Types Of Barcode Symbols

    Getting Familiar with Your TriPlus RSH Barcode Reader Supported Types of Barcode Symbols The following barcode types can be decoded. See Table Prerequisites are: • Horizontal scan line • 1D scanner Table 20. Barcode Symbologies (Sheet 1 of 2) Barcode Type...

  • Page 92: Approved Barcode Labels

    Getting Familiar with Your TriPlus RSH Barcode Reader Table 20. Barcode Symbologies (Sheet 2 of 2) Barcode Type Description Min Length Barcode Symbology Length Code 39 (3 of 9) Alphanumeric code, consisting of Unlimited uppercase letters (A-Z), numeric digits (0-9) and some special characters (-, ., $, /, +, %, and space).

  • Page 93: Reading The Barcode From A Vial Or Well Plate

    Getting Familiar with Your TriPlus RSH Barcode Reader Figure 48. Examples of Barcode Label Positioning and Label Size Reading the Barcode from a Vial or Well Plate The Barcode Reader reads a barcode label from an individual vial, but it is not able to scan and treat multiple units of information, as would typically be received from a multiple well plate.

  • Page 94: Standard Tray Holder

    Getting Familiar with Your TriPlus RSH Standard Tray Holder Table 21. Barcode Reader - Dimensions and Mass Length Dimension in mm (in.) Width 30 (1.18) Depth 273 (10.75) Height 128 (5.04) Mass Dimension in kg (lbs) Barcode Reader Module (with cable) 0.83 (1.83)

  • Page 95: Liquid Cooled Tray Holder

    Getting Familiar with Your TriPlus RSH Liquid Cooled Tray Holder The Standard Tray Holder specifications are: • Sample Capacity — 648 x 2 mL vial • Vial Size — 0.3, 0.5, 0.7, 2.0, 2.5, 10, and 20 mL and micro volume non-standard vials •...

  • Page 96: Temperature Controlled Drawer

    Getting Familiar with Your TriPlus RSH Temperature Controlled Drawer CAUTION The Liquid Cooled Tray Holder operates within a temperature range from +4 °C to 70 °C. The Liquid Cooled Tray Holder specifications are: • Sample Capacity — Holds one tray for 32 vials capacity of 10 mL, and 20 mL vials.
  • Page 97 WARNING It is mandatory to use a second Power Module. Do not use the same power supply as used for the TriPlus RSH. See the chapter Installation of the TriPlus RSH System in the TriPlus RSH Hardware Manual for details.

  • Page 98: Temperature Control

    Getting Familiar with Your TriPlus RSH Temperature Controlled Drawer Temperature Control The cooling/heating capacity of a temperature control device based on the Peltier technique is always relative to room temperature. The maximum temperature difference that can be reached between the device and ambient temperature is limited. The Temperature Controlled Drawer can attain a minimum of 14 °C (TΔ) below ambient temperature.

  • Page 99: Condensation Build-Up

    Getting Familiar with Your TriPlus RSH Temperature Controlled Drawer • Switch on the Temperature Controlled Drawer at least 30 minutes before an analytical operation at +10 °C or 75 minutes before an operation at +4 °C. • For high-throughput analyses – typical cycle time of 60 seconds or less per analysis – leave the drawers open between injections.

  • Page 100: Temperature Controlled Drawer Specifications

    Getting Familiar with Your TriPlus RSH Temperature Controlled Drawer Temperature Controlled Drawer Specifications The specifications are: • Sample Capacity — Accommodate two different sample trays. Compatible VT70, VT54, and VT 15 (10 mL vials only). • Total vial height — 47 mm (including cap) •...

  • Page 101: Electronic Box For Oc Injector Actuator

    3000 meters above sea level Electronic Box for OC Injector Actuator The OC Injector Actuator, not provided with the TriPlus RSH, is a combination of the On-column Injector and the Actuator to open and close the rotary valve of the On-Column injector.

  • Page 102: Handheld Controller

    This section provides a description of the Handheld Controller. Menu Screen Different menu screens are displayed, depending on the TriPlus RSH operating state and the particular function being accessed by the operator. All menu screens have the same basic format. The uppermost line is the Status bar where the selected section, the status and time are displayed.

  • Page 103: Menu Screen Status Bar Symbols

    Update or Backup. (For the processes Copy Backup and Restore the Status light remains green.) Blue status light, permanent: TriPlus RSH is in a safe state (power reduced to a minimum) and requires manually moving the Head to the Teach Point.

  • Page 104: Function Keys And Buttons

    The automated run is paused and the user has the possibility to interfere with, make corrections to, continue with or stop the run completely. The Stop button used with the TriPlus RSH can terminate the process during the run.

  • Page 105: Scroll Wheel And Enter Button

    Handheld Controller Versus the Virtual Handheld Controller With every TriPlus RSH, Virtual Handheld Controller Software is provided. As indicated by its name, this software replaces the actual hardware, the Handheld Controller. However, the Handheld Controller is still available as an option.
  • Page 106 Getting Familiar with Your TriPlus RSH Handheld Controller Figure 57. Example Context Menu to Enter a Password TriPlus RSH User Guide Thermo Scientific...

  • Page 107: Chapter 2 Triplus Rsh Basic Informations

    This chapter provides basic informations to start the TriPlus RSH, and for using the Handheld Controller. Basic informations are provided for the different Access Levels to start the TriPlus RSH, and for using the Handheld Controller. The use of the Handheld Controller is similar to the Virtual Handheld Controller, and the PC software-based Handheld Controller.

  • Page 108: Starting The Triplus Rsh

    Starting the TriPlus RSH This section describes how to start the TriPlus RSH. IMPORTANT Before starting the TriPlus RSH, make sure that the sampler and all the modules are properly already installed. A syringe of the appropriate type, or several syringes (Tools), named using the appropriate Method, must be installed in the TR Station or ATC Station, and positioned in the slot.
  • Page 109 The envelope symbol indicates that a message pending or a Service Issue is found. When powered up, the TriPlus RSH is always in the User access level. In this level, the message symbol is visible in the status line, but you have no access rights to verify or acknowledge the error.

  • Page 110: Short-Cuts From Startup Screen

    Figure 61. List Items of Pending Messages When an error is observed, check all connections, try to eliminate the source of the problem, and restart the TriPlus RSH again. The messages accompanied by the Exclamation Mark warning symbol indicate that a module has not been completely configured.

  • Page 111: Access Levels

    Settings which require to be changed rarely, such as those set during installation of the TriPlus RSH, are hidden for level 1 users (User Level), and revealed at level 2 (Extended User Level).

  • Page 112: Extended User Level

    RSH Firmware can be controlled, such as those that may be used for new modules, X-,Y-,Z-axes position teaching, changing a sync signal, and so on. It is highly recommended to operate the TriPlus RSH in the User Level and only for special cases to activate the Extended User Level.

  • Page 113: Service Level

    Figure 63. Example of Extended User Level Pull-up Menu Options Service Level The Service Level provides full access to the TriPlus RSH. This level is reserved for qualified service engineers only. This level will be required for installation or any major repairs such as replacing a device (active module).
  • Page 114 TriPlus RSH Basic Informations Service Level If the TriPlus RSH is equipped with a Handheld Controller, press the two Menu Buttons simultaneously (see Figure 64) to access the screen shown in Figure 65. The User Access Level is the default level.

  • Page 115: Example Of Differences In Access Levels

    Firmware in the TriPlus RSH Hardware Manual. Use a backup file which does not yet contain the password. Be aware that changes made to the TriPlus RSH or any changes in settings made after the installation of the backup file to recover the forgotten password will no longer be active.

  • Page 116: Setup, Installation, And Maintenance Menu Items

    30 minutes of inactivity the software reverts automatically to the default User Level. Please note that the TriPlus RSH is always started in the User Access level. If the Extended User mode is required, you have to change the access level. See “User Level”...

  • Page 117: About Menu Item

    This item informs you on which software versions are currently installed with the particular TriPlus RSH. See Figure Figure 70. Menu Item About Shutdown Menu Item The menu item Shutdown gives the operator the possibility to shut down the TriPlus RSH in a controlled manner. See Figure Thermo Scientific TriPlus RSH User Guide...

  • Page 118: Operation Of The Triplus System In Combination With Other Chromatography Data Systems

    • The Head moves to an empty position of the ATC Station, engages and remains in this position. • In case a TR Station is part of the TriPlus RSH configuration, the mead moves to the single slot. • The software shuts down, closes the application and disables the axes.

  • Page 119: Chapter 3 Setup Menu Item

    • Setup Menu Item Modules • Setup Menu Item - Set Date and Time • Setup Menu Item - Set TriPlus RSH Name • Setup Menu Item - Setup Network Note Items highlighted in green are only visible in the access level Extended User.

  • Page 120: Starting Setup Menu Item

    “Setup Menu Item Modules” page 95 • “Setup Menu Item - Set Date and Time” page 176 • “Setup Menu Item - Set TriPlus RSH Name” page 177 • “Setup Menu Item - Setup Network” page 178 TriPlus RSH User Guide...

  • Page 121: Setup Menu Item Modules

    Setup Menu Item Setup Menu Item Modules Setup Menu Item Modules Select the menu item modules to open the list of all installed active and passive modules. Figure Figure 73. List of Active and Passive Modules Thermo Scientific TriPlus RSH User Guide...
  • Page 122 “Tray Types” page 146 • “Trays” page 158 • “Vial Types” page 160 • “Vials” page 164 • “Wash Stations” page 168 • “Tray Slots” page 174 • “Unknown” page 174 • “Valves” page 174 TriPlus RSH User Guide Thermo Scientific...

  • Page 123: Agitator

    The Standby Temperature is typically lower than the application temperature. Vial Type Pos. 1 The Vial Type for Position 1 is displayed. Read-only. The Vial Type can be set with the task Setup Modules. Thermo Scientific TriPlus RSH User Guide...
  • Page 124 A job can also contain a single sample, often seen in method development work. In such cases, you set the Standby Temperature to the same temperature used for the application. Dropping the temperature during runs can be time-consuming and disruptive. TriPlus RSH User Guide Thermo Scientific...
  • Page 125 • Speed: Select the required speed, range from 250 rpm to 750 rpm Press Run to start the Agitator To stop the motion, press Options again, select Check Agitator and set the parameter Options to Off. Press Run. Thermo Scientific TriPlus RSH User Guide...
  • Page 126 You can change the Vial Type at any time during a routine application selecting the menu item Agitator, scrolling down to the list item Vial Type and selecting another Vial Type. Examples of Free available and Reserved Vial Positions are visualized in the following images: TriPlus RSH User Guide Thermo Scientific...

  • Page 127: Vortexer

    Setup Menu Item Setup Menu Item Modules Vortexer Upon selecting the list item Agitator & Vortexer, the following parameters are available for modification. See Figure Figure 75. Vortexer Parameters Thermo Scientific TriPlus RSH User Guide...
  • Page 128 • Speed — Select the required speed, range from 250 rpm to 2500 rpm Press Run to start the Vortexer To stop the motion, press Options again, select Check Vortexer and set the parameter Option to Off. Press Run. TriPlus RSH User Guide Thermo Scientific...

  • Page 129: Barcode Reader

    Z-positions are defined correctly. In case a teaching point is off, return to the Menu Setup for readjustment of the teaching position. Please note that this action can be done under the Extended User Level but not under User Level. Thermo Scientific TriPlus RSH User Guide...

  • Page 130: Chromatograph System

    This minimizes erroneous functions and simplifies the user interface. This is the last step in the setting up and synchronization of Input/Output Signals between the TriPlus RSH system and the external device. See also the section “Input/Output Interface” page 106, and the section “Signals”...
  • Page 131 A ready signal from another device is expected as an Input Signal. Note The synchronization between the TriPlus RSH and any chromatographic system has to be checked for each particular system configuration. The setting of the synchronization signal has to be matched with the pin allocation of the synchronization cable.

  • Page 132: Input/Output Interface

    Debounce Time Low Active Simulated Signal On Trigger Event This signal can be used for testing, simulation Waiting Time of sending a signal (Output Signal) SW Out1 Signal On Switch-out Signal, Relay contact Low Active TriPlus RSH User Guide Thermo Scientific...
  • Page 133 High Active or Low Active. Depending on what has to be applied for the specific system, one has to match the signal logic on the TriPlus RSH side. By setting a mark to Low Active means that Low Active is selected.
  • Page 134 Description Reset to Default Resets the selected module parameter to default value. Beep Actives a Beep sound local on TriPlus RSH. If activated, the operator can select the Duration, Frequency, and the Volume of the tonal sound. Duration The time for the tonal sound is selectable from 100 ms to 60 seconds.

  • Page 135: Injectors

    GC Injector. If the difference is greater than the Position Tolerance of 3.0 mm (default value) the TriPlus RSH immediately stops. This is a safety feature to avoid needle bending.

  • Page 136: Oc Actuator

    116. Note The OC Adapter is part of the GC On-column Injector and it is not provided with the TriPlus RSH. Selecting the list item OC Actuator opens up the following parameters. See Figure TriPlus RSH User Guide Thermo Scientific...
  • Page 137 Reads out the position of the OC Actuator. This information can be used for troubleshooting purposes. Homing Direction Changes the direction of homing. Homing Position Changes the position of homing. Max Ndle Penetr Depth Defines the maximum allowed syringe needle penetration in millimeters. Thermo Scientific TriPlus RSH User Guide...
  • Page 138 OCI. If the difference is greater than the Position Tolerance of 1.5 mm (default value) then the TriPlus RSH immediately stops. This is a safety feature to avoid needle bending. OC Actuator Options Pull-up Menu Selecting the pull-up menu Options opens up the following tasks.

  • Page 139: Motors

    Actual position — Reads only for information Z Motor (#) Actual position — Reads only for information Acceleration Factor Allows adjusting the acceleration of the motors. Note that a change of the Acceleration Factor does affect all the motors. Thermo Scientific TriPlus RSH User Guide...
  • Page 140 Table 45. List of Motors (Sheet 2 of 3) Item Description Default Strategy Defines of the global movement of the TriPlus RSH Head across the system platform. The following selections are possible: • Parallel — Allows simultaneously movement of all axes.
  • Page 141 Slot 2 or 3), ejects the tool, and moves to the Slot where the required tool is parked. The new tool will be mounted, and the TriPlus RSH will be ready for the next application.

  • Page 142: Obstacle

    The object Obstacle is a module from another device, for example a large Thermal Conductivity Detector (TCD). If a module exceeds a certain height, it could get in the path way of the TriPlus RSH. The dimensions and the position of the Obstacle can be defined. The TriPlus RSH will exclude this area from the allowed path way and will bypass it.
  • Page 143 Table 48. Obstacle Option Parameters (Sheet 1 of 2) Item Description New Obstacle Creates a new obstacle. Copy Obstacle Copies the present obstacle. Delete Obstacle Deletes the present obstacle. Reset to Default Resets the selected module parameter to default value. Thermo Scientific TriPlus RSH User Guide...

  • Page 144: Sample Drawers

    Note The Temperature Controlled Drawer can be adjusted through the BUS protocol. For the power supply it is mandatory to use a second Power Module. Do not use the same power supply as used for the TriPlus RSH. Selecting the list item Sample Drawers opens up the following parameters. See Figure Figure 83.
  • Page 145 To activate it, go to the pull-up menu Options and select Set Standby Temperature. The Standby Temperature is activated when the TriPlus RSH is powered up. In routine use, the Standby Temperature is usually activated after a job has been terminated.The Standby Temperature is typically around ambient temperature;...
  • Page 146 Extended User Level but not under User Level. Teach module The positions of the X-, Y-, and Z-axes can be defined again, teaching. Activate Standby Temperature This task activates the newly-set Standby Temperature. TriPlus RSH User Guide Thermo Scientific...

  • Page 147: Tool Stations

    Upon selecting the list item Tool Station, the following parameters are available for modification. See Figure 84. Press Enter to edit a parameter. Figure 84. Menu Item Tool Stations and Menu List Items for Tool Stations Thermo Scientific TriPlus RSH User Guide...

  • Page 148: Tr Station

    Upon selecting the list item ATC Station, the following parameters are available for modification. See Table Table 53. ATC Station Parameters (Sheet 1 of 2) Item Description Slot1 to Slot3 A slot can be selected to see which tool has been assigned to this particular slot. TriPlus RSH User Guide Thermo Scientific...
  • Page 149 For information only. Tool Present Indicates if the tool is parked in the Slot (if not, either mounted in the TriPlus RSH Head, or not recognized by the system). Fiber Protection This parameter allows defining the Slot Position for the SPME Tool.

  • Page 150: Signals

    Setup Menu Item Modules Signals Note Synchronization signals are inputs that tell the TriPlus RSH when to wait or to proceed with a sample-processing step. Output signals are sent from the system to external devices to indicate the status or completion of particular processing steps.
  • Page 151 Destination Selects the required Output signal for the synchronization with an external device. Usually a switch out signal (SW Out) contact is used to send the Start Signal to an external device. Thermo Scientific TriPlus RSH User Guide...
  • Page 152 Activates the selected signal for the time set with the parameter Duration. Since the Duration time is usually short, a status change in the parameter Signal ON is not visible. Reset to Default Resets the selected module parameters to default value. TriPlus RSH User Guide Thermo Scientific...

  • Page 153: Solvent Stations

    Pos1 = Large solvent reservoir bottle (100 mL volume in back position) Pos2 = Large solvent reservoir bottle (100 mL volume in middle position) Pos3 = Large solvent reservoir bottle (100 mL volume in front position) Thermo Scientific TriPlus RSH User Guide...
  • Page 154 Z-axis position as specified by the object. Solvent Station Options Pull-up Menu Selecting the pull-up menu Options opens up the following tasks. See Table TriPlus RSH User Guide Thermo Scientific...

  • Page 155: Spme Conditioning Station

    Figure 87. Menu Item and List Items for SPME Conditioning Station Press Enter to edit a parameter. See Table Table 60. SPME Conditioning Station Parameters (Sheet 1 of 2) Item Description Actual Temperature Reads out the actual temperature of the module; read-only function. Thermo Scientific TriPlus RSH User Guide...
  • Page 156 The coefficient used, if a temperature calibration has been performed. Possible values are between 0 and 2. Read-only. Temp.Offset The Offset temperature used, if a temperature calibration has been performed. Possible values are between -10 °C and +10 °C. Read-only. TriPlus RSH User Guide Thermo Scientific...

  • Page 157: Syringe Types

    Press Enter to edit a parameter. Selecting a syringe, for example a 10 μL Liquid Syringe, opens all the necessary parameters to define the syringe in geometry, and to define default values for an optimized use. See Table Thermo Scientific TriPlus RSH User Guide...
  • Page 158 Specifies the internal diameter of the syringe needle in mm. Needle Length Specifies the length of the syringe needle in mm. Needle Out Dia Specifies the external diameter of the syringe needle in mm. Plunger Type Specify the syringe suppliers specific. TriPlus RSH User Guide Thermo Scientific...
  • Page 159 Export Syringe Type Exports the present Syringe Type template to a USB stick. Export All Exports all Syringe Type templates at once to a USB stick. Delete Syringe Type Deletes the present Syringe Type template. Thermo Scientific TriPlus RSH User Guide...

  • Page 160: Tool Adapters

    Selecting the pull-up menu Options opens up the following tasks. See Table Table 65. Tool Adapter Options Parameters (Sheet 1 of 2) Item Description New MHE Station Creates a new MHE Station. Copy MHE Station Copies the present MHE Station. TriPlus RSH User Guide Thermo Scientific...

  • Page 161: Tools

    Figure 90. Menu Item Tools and Menu List Items Tools • Figure 91 shows the Syringe Type selection for HS, LS, and SPME Tools. • Figure 92 shows the Syringe Type selection for ITEX Tool. Thermo Scientific TriPlus RSH User Guide...
  • Page 162 Setup Menu Item Setup Menu Item Modules Figure 91. Syringe Type Selection for HS, LS, and SPME Tools SPME Figure 92. Syringe Type Selection for ITEX Tool ITEX Press Enter to edit a parameter. See Table TriPlus RSH User Guide Thermo Scientific...
  • Page 163 To activate it, go to the pull-up menu Options and select Set Standby Temperature. The Standby Temperature is activated when the TriPlus RSH is powered up. In routine use, the Standby Temperature is activated after a job has been terminated. The Standby Temperature is typically lower than the application temperature.
  • Page 164 To activate it, go to the pull-up menu Options and select Set Standby Temperature. The Standby Temperature is activated when the TriPlus RSH is powered up. In routine use, the Standby Temperature is activated after a job has been terminated. The Standby Temperature is typically lower than the application temperature.
  • Page 165 To activate it, go to the pull-up menu Options and select Set Standby Temperature. The Standby Temperature is activated when the TriPlus RSH is powered up. In routine use, the Standby Temperature is activated after a job has been terminated. The Standby Temperature is typically lower than the application temperature.
  • Page 166 Parks a tool without picking up another tool from the ATC Station. Configure SPME Tool This line is visualized when a SPME tool is selected. This task allows to configure a SPME tool. Fiber adjustment. TriPlus RSH User Guide Thermo Scientific...

  • Page 167: Naming Convention And Relationship Between Software Classes' Tray Holder, Trays, Tray Types, And Cap Type

    The software classes are labeled in red. All related software classes where a Vial is used are displayed using a green shade and the classes related to Well Plates are displayed using a blue shade. Thermo Scientific TriPlus RSH User Guide...

  • Page 168: Tray Holders

    Tray Holders are passive modules. These modules are described by their geometric design parameters, but there is no control whatsoever through the TriPlus RSH. A Tray Holder can be a Liquid Cooled Tray (using a circulation bath for heating/cooling), or a Tray Holder without any temperature control.
  • Page 169 If no Tray is assigned yet, use the button New to assign a Tray to a particular Slot. The dialog for naming a Tray can be started by pressing the Enter button. See the examples. Figure Thermo Scientific TriPlus RSH User Guide...
  • Page 170 Figure 95. Tray Type and Vial Type Selections to Assign to Tray • Slot(#) — Select the required Slot number to assign a Tray Type and Vial Type. • Tray(#) — Select the required Tray (Rack) number to assign the Tray Type and Vial Type. TriPlus RSH User Guide Thermo Scientific...
  • Page 171 Table 69. Tray Holder Option pull-up Menu Parameters When Tray(#) is Selected (Sheet 1 of 2) Item Description New Tray (New Rack) Allows creating a new Tray (Rack). New Well Plate Allows creating a new well plate. Delete Tray Type Allows deleting the present Try Type. Thermo Scientific TriPlus RSH User Guide...

  • Page 172: Tray Types

    Parameters such as how many sample vials are in a column and in a row, and the distance from vial to vial are all summarized in this class for each Tray Type. Selecting the list item Tray Types opens up the following parameters. See Figure TriPlus RSH User Guide Thermo Scientific...
  • Page 173 Y-axis; in a Well Plate the numbering order is by the X-axis. Tray type R32 LiqCooled Well Plate type MTP96 Press Enter to edit a parameter. See Table Thermo Scientific TriPlus RSH User Guide...
  • Page 174 Total length of the Tray in Z-axis direction, outer frame of Tray is considered. Unit: mm. Penetration Vial Index Specifies the position in the Tray where the cut-out for needle penetration adjustment is located. Rows Number of rows. TriPlus RSH User Guide Thermo Scientific...
  • Page 175 The adapter installed plus the vial height must not exceed the total height of a 20 mL vial. Example: 10 mL vial plus corresponding adapter must be equal to the height of a 20 mL vial. Thermo Scientific TriPlus RSH User Guide...
  • Page 176 (Parameter used for MTP only) Bottom Sense supported Setting the mark to Bottom Sense supported enables the functionality of needle bottom sensing. (Parameter used for MTP only) The tray type parameters are described in the following Table TriPlus RSH User Guide Thermo Scientific...
  • Page 177 Definition of Row and Column Example: Regular order of vials. Rows — Number of Rows Figure 99. Tray type MTP 96 Columns — Number of Columns Definition of Row and Column Example: Regular order of wells. Thermo Scientific TriPlus RSH User Guide...
  • Page 178 Definition of Row and Column Example: Staggered order of vials. Indexing Orientation Figure 102. Indexing orientation Direction X (or Row Direction with RSH Firmware 1.4.0 or lower) Direction Y (or Column Direction with RSH Firmware 1.4.0 or lower) TriPlus RSH User Guide Thermo Scientific...
  • Page 179 1st Sample Offset X Figure 104. Tray type VT54 1st Sample Offset Y (1st Sample Offset Z) Alt Sample Offset X Figure 105. Tray type R60 Alt Sample Offset Y (Alt Sample Offset Z) Thermo Scientific TriPlus RSH User Guide...
  • Page 180 Figure 107. Tray type R32 Liquid Cooled Length X Length Y Length Z Total Column Length X Total Row Length Y Length X Figure 108. Tray type VT54 Length Y Length Z Total Column Length X Total Row Length Y TriPlus RSH User Guide Thermo Scientific...
  • Page 181 Tray Type Parameter Illustration Cavity Depth Figure 109. Tray type MTP96 Cavity Diameter Penetration Vial Index Figure 110. Tray type VT54 Z Tolerance Figure 111. Tray type R60 Z Tolerance Figure 112. Tray type MTP96 Thermo Scientific TriPlus RSH User Guide...
  • Page 182 Slot Offset X Figure 115. Tray Holder with Tray Type R60 inserted Slot Offset Y Note that the value has a negative sign if the Tray is oversize, which means larger than the Tray Holder. TriPlus RSH User Guide Thermo Scientific...
  • Page 183 This task deletes the present Tray Type (for example VT54). Reset to Default Resets the selected Tray Holder parameter to default value. Note There are no other pull-up menu items available. Currently it is not possible to create custom Tray Types. Thermo Scientific TriPlus RSH User Guide...

  • Page 184: Trays

    Selecting the pull-up menu Options opens up the following tasks. See Table Table 74. Tray Option Pull-up Menu Parameters Item Description New Tray Creates a new Tray Holder. Copy Tray Copies the present Tray. Delete Tray Deletes a present Tray. TriPlus RSH User Guide Thermo Scientific...
  • Page 185 The position of cut-out in the Tray is specified in the Tray Type, therefore is the position known. Starting this task, moves the TriPlus RSH Head to the defined vial position where it waits for the manual adjustments. See Figure 120, illustrating the cut-out in the Tray.

  • Page 186: Vial Types

    Within the same class are the same list items available. The three classes are shown in detail for the 10-CV Vial type, the 100SR Wash Vial Type, and for the Waste Vial Waste Vial Type. Selecting the list item Vial Types opens up the following parameters. See Figure 121. TriPlus RSH User Guide Thermo Scientific...
  • Page 187 Table Table 76. Vial Types Parameters (Sheet 1 of 3) Item Description Default Needle Penetration The default value of the syringe needle penetration depth in Depth the reservoir vial is set by this parameter. Thermo Scientific TriPlus RSH User Guide...
  • Page 188 45 mm or 73 mm. Penetration Speed The syringe needle penetration speed is set by this parameter. It is recommended to use the default value first. Changing this parameter in either direction might lead to needle bending. TriPlus RSH User Guide Thermo Scientific...
  • Page 189 Description Strip Distance When a magnetic cap is used, the distance to move the TriPlus RSH Head to the side (in the Y-direction) to wipe off the vial can be defined. Measured in mm. Volume This parameter defines the volume of the vial.

  • Page 190: Vials

    In the case when there is no Vial (Solvent Reservoir, Wash Vial or Waste Vial) available, the command New Vial is available with the Menu Button Option. Follow the dialog with the known possibilities to assign a name as shown in Figure 123. TriPlus RSH User Guide Thermo Scientific...
  • Page 191 The parameters available for the Vial Type reservoir are slightly different from the parameters for the Wash Vial and Waste Vial. See the examples in Figure 124 Figure 125, and the description in Table Thermo Scientific TriPlus RSH User Guide...
  • Page 192 45 mm or 73 mm. Def Nedl Penetr Depth The default value of the syringe needle penetration depth in the reservoir vial is determined by this parameter. TriPlus RSH User Guide Thermo Scientific...
  • Page 193 Z-axis position as specified by the object. Vial Option Pull-up Menu Selecting the pull-up menu Options opens up the following tasks. See Table Thermo Scientific TriPlus RSH User Guide...

  • Page 194: Wash Stations

    The positions of the X-, Y-, and Z-axes can be defined again, (teaching). Wash Stations Three different wash stations are available with the TriPlus RSH: Standard Wash Station (passive module), Large Solvent Wash Station (passive module), and Fast Wash Station (active module).
  • Page 195 Penetration Speed The syringe needle penetration speed is set by this parameter. It is recommended to use the default value first. Changing this parameter in one or the other direction might lead to needle bending. Thermo Scientific TriPlus RSH User Guide...
  • Page 196 Table 81. Parameters for Fast Wash Stations (Sheet 1 of 3) Item Description Wash 1 to Wash 2 Wash positions 1 and 2 can be assigned to Fast Wash Station. Waste Defines the Waste position. TriPlus RSH User Guide Thermo Scientific...
  • Page 197 12 mm due to needle guide, and so on. For a typical needle length of 57 mm or 85 mm for the long needle, the maximum penetration depth must not exceed 45 mm or 73 mm. Thermo Scientific TriPlus RSH User Guide...
  • Page 198 • Waste Port — Large Wash and Fast Wash Module • Waste Vial — Standard Wash. • Waste Inset — Standard Wash with inserted Drainage Adapter. Volume Defines the volume of the reservoir vial. TriPlus RSH User Guide Thermo Scientific...
  • Page 199 Extended User Level but not under User Level. New Wash Station Creates of a new Wash Station. Prime Wash Liner Primes the tubing of the selected Wash Position. Duration Sets the time required for priming. Thermo Scientific TriPlus RSH User Guide...

  • Page 200: Tray Slots

    Figure 127. Menu Item Tray Slots Unknown This menu List Item will be implemented in future. See Figure 128. Figure 128. Menu Item Unknown Valves This menu List Item will be implemented in future. See Figure 129. TriPlus RSH User Guide Thermo Scientific...
  • Page 201 Setup Menu Item Modules Figure 129. Menu Item Valves The Menu Items which are not part of the system configuration, showing (0) behind the class name, are not listed in the User access level. Thermo Scientific TriPlus RSH User Guide...

  • Page 202: Setup Menu Item - Set Date And Time

    Table 84. Date and Time Format Item Description Set Date and Time Date: format yyyy-mm-dd Time: format hh:mm:ss Time Zone: Greenwich Mean Time (GMT) plus or minus the hour deviation from Greenwich standard time. TriPlus RSH User Guide Thermo Scientific...

  • Page 203: Setup Menu Item - Set Triplus Rsh Name

    The name has to start with an alphabetical character. 3. Set a unique name for the TriPlus RSH. This name will be stored and can be used for identification of the system.

  • Page 204: Setup Menu Item - Setup Network

    In order to select the required network setup according to your laboratory setting, the following possibilities are available: • Direct connection — The TriPlus RSH is directly connected to the computer via the LAN cable. There is no corporate network or router connection.
  • Page 205 Communication within the network is provided by a hub or switch, defined as Gateway. Default Gateway is 192.168.99.1 Note For more information see Setting up the Network Interface in the TriPlus RSH Hardware Manual. Thermo Scientific TriPlus RSH User Guide...

  • Page 207: Chapter 4 Maintenance Menu Items

    Handheld Controller, or directly using the installed Handheld Controller. Contents • Maintenance Menu • Check Tool Calibration • Check Teaching Module • Check Configuration • Reset Configuration • Create Diagnostic Backup • Create Configuration Backup • Copy Backup • Restore Backup Thermo Scientific TriPlus RSH User Guide...

  • Page 208: Maintenance Menu

    184 • “Reset Configuration” page 184 • “Create Diagnostic Backup” page 185 • “Create Configuration Backup” page 186 • “Copy Backup” page 187 • “Restore Backup” page 188 • “Import Templates” page 188 TriPlus RSH User Guide Thermo Scientific...

  • Page 209: Check Tool Calibration

    Follow the dialog shown on the display of the Handheld Controller, or on the screen of the Virtual Handheld Controller. Details are described in TriPlus RSH Check Tool Calibration in the TriPlus RSH Hardware Manual. Check Teaching Module Selecting Check Teaching modules the TriPlus RSH Head moves from one module to the next, in the order listed in the table modules.

  • Page 210: Check Configuration

    Note that the messages can be opened and viewed in the Extended User access level only. Reset Configuration By selecting Reset Configuration the RSH Firmware (Application) resets the configuration to a standard application package. TriPlus RSH User Guide Thermo Scientific...

  • Page 211: Create Diagnostic Backup

    (Used in Service Installation Wizard Calibrate Tool.) Create Diagnostic Backup By using the command Create Diagnostic Backup, essential information from the current status of the TriPlus RSH is extracted, and saved in an encrypted form on the SD card. Thermo Scientific TriPlus RSH User Guide...

  • Page 212: Create Configuration Backup

    Diagnostic Backup file, with extension ‘*.pack, does take approximately eight minutes. The size of the file is in the range of 12 MB, depending on the configuration of the TriPlus RSH. The Diagnostic Backup file can also be used for a Restore procedure.

  • Page 213: Copy Backup

    To copy a configuration backup from the SD card, insert the USB stick into the connector USB Host on the back of the TriPlus RSH. Refer also to the chapter RSH Firmware in the TriPlus RSH Hardware Manual. Thermo Scientific...

  • Page 214: Restore Backup

    Restore Backup Restore Backup The function Restore allows loading a Backup file saved from the same TriPlus RSH system back to the system. Only a Backup file (*.pack file) can be used. The key, the encryption of the Application, and the device Firmware files must match.

  • Page 215: Chapter 5 Service Menu Item

    Handheld Controller, or directly using the installed Handheld Controller. Contents • Service Menu • Installation Item • Create New Tray System Item • Teach Home Position Note Items highlighted in green are only visible in the access level Extended User. Thermo Scientific TriPlus RSH User Guide...

  • Page 216: Service Menu

    “Access Levels” page Figure 134. Changing Access Level When the TriPlus RSH is powered up the first time, the Message Envelope is shown in the Menu Screen Status Bar. See the example in Figure 134. The reason for this message is that a TriPlus RSH cannot be operated as long as the motors are not calibrated.

  • Page 217: Installation Item

    Motors has been completed. Each point ticks off as it is completed. When the TriPlus RSH is powered on for the very first time, these wizard items which have to be accomplished are active (black). The grayed out wizard items will be active as soon as the system is calibrated (Calibrate Motors) and if the hardware configuration of the TriPlus RSH requires these points.
  • Page 218 Figure 136. Installation Wizard. The twelve points to cover are: • “Set Regional Settings” page 193 • “Set Date and Time” page 194 • “Set TriPlus RSH Name” page 195 • “Setup Network” page 196 • “Calibrate Motors” page 199 •...

  • Page 219: Set Regional Settings

    IMPORTANT When a Set Regional Setting parameter is changed, a reboot of the system is required. Please note that the Regional Settings are active for the Handheld Controller only, the Virtual Handheld Controller is dependent on the settings of the computer operating system. Thermo Scientific TriPlus RSH User Guide...

  • Page 220: Set Date And Time

    Table 89. Set Date and Time Parameters Item Description Date The format is: yyyy/mm/dd Time The format is hh:mm:ss Time Zone Greenwich Mean Time (GMT) plus or minus the time deviation (in hours) from Greenwich standard time. TriPlus RSH User Guide Thermo Scientific...

  • Page 221: Set Triplus Rsh Name

    Installation Item Set TriPlus RSH Name  To set TriPlus RSH name 1. Select the wizard item Set TriPlus RSH Name to set a name for the TriPlus RSH for identification. See Figure 139 Table Figure 139. Wizard Item Set TriPlus RSH Name 2.

  • Page 222: Setup Network

    Table 90. Set TriPlus RSH Name Parameter Item Description Set TriPlus RSH Name Set a unique name for the TriPlus RSH. This name will be stored and is used for identification of the system. Insert Character Allows inserting a character. Press Enter to receive the selection of capital letters.
  • Page 223 1. Check the connections of the LAN cable. The default addresses is visible after reentering the Setup Network screen. 2. Check the LEDs on the Ethernet connector at the back of the TriPlus RSH. Thermo Scientific TriPlus RSH User Guide...
  • Page 224 In order to select the required network setup according to the laboratory setting, the following possibilities are available: • Direct connection — The TriPlus RSH is directly connected to the computer via the LAN cable. There is no corporate network or router connection.

  • Page 225: Calibrate Motors

    Figure 141. Wizard Item Calibrate Motors and Starting Calibration Wizard Step 1 of 6 Press Calibrate to start the first step Calibrate Axes. All axes from the TriPlus RSH are calibrated. Wait until the yellow light stops flashing and Next becomes available.
  • Page 226 3. When finished, press Next again to Calibrate Plunger Sensor (Steps 3). 4. Step 4 calibrates the coupling of a tool. The wizard text explains that the head must be moved above the Coupling Pivots. TriPlus RSH User Guide Thermo Scientific...
  • Page 227 Service Menu Item Installation Item Note Please note that the TriPlus RSH Head has to be positioned such that the receiving holes of the Z-Slider are above the Coupling Pivots of the tool but they must not be engaged. Upon starting the wizard, the TriPlus RSH automatically turns off the current to enable a manual movement of the TriPlus RSH Head.

  • Page 228: Teach Tool Station

     To proceed with different types or configurations of the Tool Station The TriPlus RSH system can be equipped with a TR Station or with one or two ATC Stations. The following configurations are allowed: • One TR Station •...
  • Page 229 3. When the TriPlus RSH is powered off, connect the supplied control cable from the rear side of ATC Station #1 to the connector marked Bus on the TriPlus RSH control interface. If Bus connector is already occupied, connect the cable to another Active module with an open connector.
  • Page 230 If this is not observed, an error message will appear. The wizard Teach Tool Station calibrates the position of the slot(-s). The height of the tool, installed in the slot, is calibrated against the TriPlus RSH Injection Unit base plate to define the coupling distance. ...
  • Page 231 Service Menu Item Installation Item 2. Step 1 of 4 requests the installation of a tool if one is not mounted to the TriPlus RSH Injection Unit. See Figure 143. Figure 143. Mount a Tool 3. When a tool has already been installed, the wizard starts with Step 1. See Figure 144.
  • Page 232 Service Menu Item Installation Item Figure 145. Teach Slot 2 and Slot 3 5. The last step moves the TriPlus RSH Injection Unit back to the Home Position. Figure 146. Figure 146. Step 4 to finish Wizard TriPlus RSH User Guide...

  • Page 233: Configure Spme Tool

    1. Select the wizard item Configure SPME Tool. See Figure 147. Figure 147. Wizard Item Configure SPME Tool 2. When a tool other than the SPME Tool is installed in the TriPlus RSH Injection Unit, the wizard asks for a tool change. Thermo Scientific TriPlus RSH User Guide...
  • Page 234 Fiber Standby Position are given in Table 150 Figure 150. Fiber Alignment and Fiber Standby Position SPME Type Fiber Alignment Fiber Standby Position SPME 1 10.8 mm 2.0 mm SPME 2 3.4 mm 2.0 mm TriPlus RSH User Guide Thermo Scientific...

  • Page 235: Set Reference Point

    Figure 151. Wizard Item Set Reference Point CAUTION It is advisable to hold with one hand the Y-Axis and with the second hand the tool body to move in a controlled way the TriPlus RSH Injection Unit to the teaching position.

  • Page 236: Calibrate Tools

    CAUTION It is advisable to hold the Y-Axis with one hand and with the other hand hold the tool body to move the TriPlus RSH Injection Unit in a controlled way to the teaching position.
  • Page 237 MTP or DW-Plate before the syringe needle penetrates the well. NonMagn2mL The lower Needle Guide has no magnets installed; a magnetic ring is not connected to it. Typically used for Microtiter Plates or for vials with a non-magnetic cap. Thermo Scientific TriPlus RSH User Guide...
  • Page 238 Slot 1 and consecutively to Slot 2 and Slot 3. Figure 154 Figure 155. Figure 154. Preparing Tool Change for Step 2, Check Tool and Pick HS Tool Figure 155. Tool Change to Prepare for Step 3 and Calibrating HS Tool TriPlus RSH User Guide Thermo Scientific...

  • Page 239: Setup Module

    Setup Module This allows you to predefine the parameters from a module. Typically, this process is done once at first installation of the TriPlus RSH. See Figure 156. Figure 156. Wizard Item Setup Module ...

  • Page 240: Teach Module

    1. Select the wizard item Teach Module. A dialog will guide the operator through this teaching wizard. Upon starting the wizard, the TriPlus RSH automatically turns off the current to enable a manual movement of the TriPlus RSH Injection Unit to the various modules configured with the system. See Figure 157.
  • Page 241 Figure 157. Wizard Item Teach Module 2. If the wizard is started without an attached tool, it first moves the TriPlus RSH Injection Unit to the first slot of the ATC Station or TR Station, picks up the provided tool, and waits for the next command.
  • Page 242 Table Table 94. Teach Modules Steps (Sheet 1 of 3) Steps Agitator Teaching Position Vortexer Teaching Position Barcode Reader Teaching Position SPME Conditioning Station Teaching GC Injector Teaching Position OC Actuator Teaching Position Position TriPlus RSH User Guide Thermo Scientific...
  • Page 243 Detector (TCD) from a GC system. If a module exceed a certain height, it could be get in the path way of the TriPlus RSH. The dimension and the position of the obstacle can be defined. The TriPlus RSH excludes this area from the allowed path way and bypasses it.

  • Page 244: Check Teaching Modules

    The pull-up menu Previous moves one step back to the previous module and verifies the teaching position again. Check Teaching Modules This section allows to check and verify the teaching positions of all the modules included into the system. TriPlus RSH User Guide Thermo Scientific...
  • Page 245 Figure 158. Wizard Item Check Teaching Modules 2. Press Check to activate the wizard. The TriPlus RSH Head moves to the first module in the list, positions itself at the Teaching Point, and waits for the next command. This gives the operator the chance to verify the position, and if necessary make some fine tuning and save the new settings.

  • Page 246: Create A Backup File

    Create a Backup File It is highly recommended to backup the configuration of system after completing the Installation wizard. Backup, Restore, and Updates are explained in chapter Interfacing the TriPlus RSH to Other Devices in the TriPlus RSH Hardware Manual. ...
  • Page 247 This file can be saved on the USB Stick, see the chapter “Setup Menu Item” page The file can also be used to make a Restore of the Control Software. Please note this option is only available with access level rights for the Extended User. Thermo Scientific TriPlus RSH User Guide...

  • Page 248: Create New Tray System Item

    2. By opening the individual list items pre-selected parameters are available for use. Figure 162. Figure 162. Tray Holder Choices Note The Tray can be either a Tray or a Well Plate. Accordingly, the choices are preselected for Tray Type or a Well Plate Type. TriPlus RSH User Guide Thermo Scientific...
  • Page 249 Vial Type 1-CV, 2-CV, 10-CV, 20-CV Regular Vials. 2-SR, 10-SR. Vials combined with corresponding adapter, reservoir. Number of Racks Defines the numbers of sample trays that can be placed in the Tray Holder. Thermo Scientific TriPlus RSH User Guide...

  • Page 250: Teach Home Position

    Teach Home Position This task is not part of the Service | Installation wizard but it must be discussed with the user during the installation process. Access to this task by entering the module RobotArmLeft. The Home Position is the position the head is moved to at the end of a task, for example when it returns to standby.
  • Page 251 3. Under the screen RobotArmLeft select the pull-up menu Options | Teach Home Position | Select. 4. Move the head to the required position and save the X-, Y-, Z- Axes Positions as the new Home Position. Press Ok to leave this wizard. Thermo Scientific TriPlus RSH User Guide...
  • Page 252 Service Menu Item Teach Home Position TriPlus RSH User Guide Thermo Scientific...

  • Page 253: Chapter 6 Analytical Troubleshooting

    This chapter gives a quick overview of possible causes and recommended actions which can be taken to eliminate an erratic behavior. Contents • General Points Regarding Chromatographic Effects • Headspace Technique Points Regarding Chromatographic Effects This guide does not replace the troubleshooting guides for chromatographic techniques. Thermo Scientific TriPlus RSH User Guide...

  • Page 254: General Points Regarding Chromatographic Effects

    How to remove a syringe with a bent needle or plunger is explained in the section Manually Removing a TriPlus RSH Tool from the RSH Head of the TriPlus RSH Hardware Manual. No sample liquid is injected. Check, adjust, the needle penetration into sample vial.
  • Page 255 Solvent level in Waste vial to high. Check the volume of the solvent in the Waste Vial. Check also the needle penetration into the waste vial. The needle must not touch the liquid at all. Thermo Scientific TriPlus RSH User Guide...

  • Page 256: Headspace Technique Points Regarding Chromatographic Effects

    Possible Cause Recommended Action Unexplained collisions of Head. Object not defined correctly. Define Object correctly. See Chapter 5 TriPlus RSH Defining Object Positions in the TriPlus RSH Hardware Manual. Verify the position for all objects, including passive and active modules.
  • Page 257 Agitator temperature heater, over temperature fuse or sensor broken. SPME Fiber Conditioning Module temperature heater or sensor broken. ITEX syringe cartridge (holder) temperature heater, over temperature fuse or sensor broken. ITEX Trap temperature heater or sensor broken. Thermo Scientific TriPlus RSH User Guide...

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