Flushing The Sample Transfer Line, Sample Tube, And Api Probe; Figure 27. Apci Source Dialog Box - Thermo Scientific LTQ Series Hardware Manual

Flushing the Sample Transfer Line, Sample Tube, and API Probe

On Standby
Thermo Scientific
Flush the sample transfer line, sample tube, and API probe at the end of each work day (or
more often if you suspect they are contaminated) with a 50:50 methanol/water solution from
the LC system through the API source at a flow rate of 200–400 μL/min for approximately
15 minutes to remove contamination.
 To flush the sample transfer line, sample tube, and API probe
1. Complete all data acquisition, if any.
2. Open the Tune Plus window (see
3. Make sure that the On/Standby button indicates the On mode, and then do one of the
following:
• If operating in APCI or APPI mode, go to
• If operating in ESI mode, go to
4. To flush the APCI source:
a. Choose Setup > APCI Source to open the APCI Source dialog box

Figure 27. APCI Source dialog box

b. In the Vaporizer Temp (°C) box, enter 500.
c. In the Sheath Gas Flow Rate (arb) box, enter 30.
d. In the Aux Gas Flow Rate (arb) box, enter 5.
e. In the Sweep Gas Flow Rate (arb) box, enter 0.
f. In the Discharge Current (μA) box, enter 0.
page 34).
step 4.
step 5.
4
Daily Operation
After Operating the LTQ Series System
(Figure 27).
LTQ Series Hardware Manual 49