Thermo Scientific TRACE 1600 Hardware Manual page 108

2
Performing Routine Maintenance
Replacing a Column
76 TRACE 1600/1610 Hardware Manual
a. Unwind the column enough to easily connect its ends to the injector and the
detector..
Note Wear clean, lint- and powder-free gloves when you handle the column and
injector ferrule.
b. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
c. Insert the column through the proper injector retaining nut and ferrule (open end
up). If the M4 retaining nut is used, slide it on the column through the side cut.
Wipe the column again with a tissue soaked in methanol.
d. Use a scoring wafer to score and break the column about 1 cm (0.4 in.) from the end.
Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
Tip Slide a notched septum on the column before the injector retaining nut to
make it easier to measure the proper distance between the nut and end of the
column.
e. Position the column so that the end of the column extends the proper distance above
the end of the ferrule as reported in
Table 13. Column Insertion Depth For SSL, SSLBKF, HeS-S/SL, PTV, PTVBKF, and GSV
Injectors
Injector
SSL and HeS-S/SL
SSLBKF
PTV
PTVBKF
GSV
f. Insert the notched septum on the column to hold the retaining nut at this position.
Thread the retaining nut into the injector but do not tighten.
g. Adjust the column position so that the septum contact the bottom of the retaining
nut.
h. Finger-tighten the retaining nut until it starts to grip the column plus a quarter turn.
i. Remove the notched septum from the column.
4. Setup the GC parameters.
Table 13.
Column Insertion Depth
• 5 mm (splitless)
• 5 mm (He saver
• 10 mm (split)
• 5 mm (splitless)
• 10 mm (split)
• 30 mm
• As far as possible into the bottom
when the PTV is used as an
On-Column injector.
• 30 mm
• Insert the column as far as goes and
withdrawn about 2 -3 mm
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