Thermo Scientific TRACE 1600 Hardware Manual page 403

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a. Use a scoring wafer to score and break the column in order to remove the current
ferrule and the nut.
17. Connect the column to the injector.
a. Wipe about 100 mm (4 in.) of the column with a tissue soaked in methanol.
b. Insert the column through the injector retaining nut and the proper ferrule (open end
up). Wipe the column again with a tissue soaked in methanol.
c. Use a scoring wafer to score and break the column about 1 cm (0.4 in.) from the end.
Use a magnifying glass to check for an even, flat cut. Repeat if necessary.
Tip Slide a notched septum on the column before the injector retaining nut to
make it easier to measure the proper distance between the nut and the end of the
column.
d. Position the column so that the end of the column extends the proper distance of
30 mm above the end of the ferrule.
e. Insert the notched septum on the column to hold the retaining nut at this position.
Thread the retaining nut into the injector but do not tighten.
f. Adjust the column position so that the septum contact the bottom of the retaining
nut.
g. Finger-tighten the retaining nut until it starts to grip the column plus a quarter turn.
h. Remove the notched septum from the column.
18. Open the gas supplies.
19. Power on the GC.
a. Plug the power cable into the AC Input connector on the back of the GC and into
the wall outlet.
b. Flip out the power switch (breaker) to the position I.
20. Setup the GC.
a. Set the oven and injector temperature to 50 °C.
b. Use the column-flowmeter connector to verify that there is flow through the column.
If you do not have a flowmeter, dip the column outlet in a small vial of methanol.
Bubbles indicate there is flow through the column. If there is no flow, check that the
carrier gas is on, the GC inlet is pressurized, and the column is not plugged. If there is
still no flow, consult the section Analytical Troubleshooting in the TRACE
1600/1610 User Guide, or contact the Technical Support.
c. Allow the column to purge for few minutes.
21. Perform a column leak check.
a. Carefully push the capillary column end into the column section of the
column-flowmeter connector. See
Installing the HS Adapter Kit on the SSL/SSLBKF Injector
Figure 356.
TRACE 1600/1610 Hardware Manual
8
Installing Optional Kits
371