Replacing A Column - Thermo Scientific TRACE 1600 Hardware Manual

Replacing a Column

Thermo Scientific
a. Plug the power cable to the AC Input connector into the back of the GC and to the
wall outlet.
b. Flip up the power switch (breaker) to the position I.
c. Set the normal injector, detector and GC working conditions.
You might need to replace the column when performance degrades and troubleshooting
indicates that the column needs maintenance. That might mean that the end of the column
needs to be trimmed, or the column needs to be replaced.
WARNING-BURN HAZARD: The injector and the oven could be hot. Cool to room
temperature before touching them.
Note Some of the cleaning procedures in this section require the use of methanol.
If methanol is unavailable or prohibited, substitute LCMS-grade or GC-grade ethanol
or isopropyl alcohol. Do not use denatured ethanol as it may contain impurities that
contaminate the GC-MS system.
 To replace a column
Note If you are running samples, stop the acquisition.
1. Remove the current column.
a. Press the Maintenance button to cool down the GC.
b. Set off the carrier and detector gases of the channel of interest, and wait for the carrier
pressure to go to zero.
c. Open the front door of the GC.
d. Unscrew the injector and detector nuts, and remove the column.
e. Remove the column from the column rack, and from the GC.
2. Install the new column.
a. Place the new column on the two arms of the column rack inside the oven.
3. Connect the new column to the injector inside the GC.
Note PTV and PTVBKF injectors mount as standard the 0.55 mm ID
terminal bottom fitting for the 0.25 mm/0.32 mm ID capillary columns
(P/N 35008428). For connecting a 0.53 mm ID capillary column, replace
the standard terminal bottom fitting with the 1.0 mm ID terminal bottom
fitting (P/N 35008429) provided in the PTV/PTVBKF standard outfit.
2
Performing Routine Maintenance
Replacing a Column
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