Thermo Scientific thermoscientific Vanquish Pumps C Operating Manual
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Thermo Scientific thermoscientific Vanquish Pumps C Operating Manual

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thermo scientific
Vanquish
Pumps C, Pumps F
VC-Pxx, VF-Pxx
Operating Manual
4820.4405-EN Revision 4.0
•
December 2019

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Summary of Contents for Thermo Scientific thermoscientific Vanquish Pumps C

  • Page 1 Vanquish Pumps C, Pumps F VC-Pxx, VF-Pxx Operating Manual 4820.4405-EN Revision 4.0 • December 2019...
  • Page 2 Copyright © 2019 Thermo Fisher Scientific Inc. All rights reserved. Original Operating Manual The hardware descriptions in this manual revision refer to devices VC-P10-A-01, VC-P20-A-01, VC-P21-A-01, VC-P32-A-01, VC-P33-A-01, VC-P40-A-01, VF-P10-A-01, VF-P20-A, VF-P32-A-01. Trademarks Acrobat, Adobe, and Adobe Reader are trademarks of Adobe Systems Incorporated. Microsoft and Windows are trademarks of Microsoft Corporation.
  • Page 3 Contacting Us Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact your local Thermo Fisher Scientific sales organization. For contact information, go to Contact Us on http://www.thermofisher.com. Technical Assistance For technical support for HPLC products, contact your local Thermo Fisher Scientific support organization.
  • Page 4 Contacting Us Page 4 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 5: Table Of Contents

    Contents Contents 1 Using this Manual .............. 11 About this Manual .................... 12 Conventions...................... 13 1.2.1 Safety Messages.................. 13 1.2.2 Special Notices and Informational Notes .......... 13 1.2.3 Typographical Conventions .............. 14 Reference Documentation ................... 15 2 Safety.................. 17 Safety Symbols and Signal Words................. 18 2.1.1 Safety Symbols and Signal Words in This Manual ........
  • Page 6 Contents Mixing System ...................... 37 Leak Detection...................... 38 Operation ...................... 39 4 Unpacking ..................  41 Unpacking...................... 42 Scope of Delivery.................... 44 5 Installation.................  45 Safety Guidelines for Installation ................. 46 Installing the Device ..................... 47 Site Requirements .................... 49 5.3.1 Power Considerations ................ 49 5.3.2 Power Cord.................... 50 5.3.3 Condensation ...................
  • Page 7 Contents 6 Operation...................  83 Introduction to this Chapter................. 84 Safety Guidelines for Operation ................ 85 Control Elements .................... 86 6.3.1 Keypad......................  86 6.3.2 Status Indicators.................. 88 Power On/Off Control .................. 91 Use of Solvents and Additives ................ 92 Preparing the Device for Operation .............. 95 Important Operating Parameters................. 97 Purging the Pump ....................
  • Page 8 Contents Pump Head ...................... 136 7.6.1 Overview of Pump Head Parts ...............  137 7.6.2 Replacing the Pump Head .............. 138 7.6.3 Replacing the Pistons ................ 144 7.6.4 Cleaning the Piston.................  146 7.6.5 Replacing the Piston Seals or Support Ring.......... 147 7.6.6 Recommendations for New Piston Seals..........
  • Page 9 Contents 8 Troubleshooting...............  197 General Information about Troubleshooting ............  198 Messages ...................... 200 Testing the Pump for Leakage ................ 208 Resolving Liquid Leaks .................. 209 Pressure Issues or Shifts in Retention Time............ 210 8.5.1 Pressure Not Zero with Open Purge Valve.......... 210 8.5.2 Checking the Compression Values ............
  • Page 10 Contents 11 Specifications ................ 253 11.1 Performance Specifications ................ 254 11.1.1 Quaternary Pump and Dual Pump ............ 254 11.1.2 Binary Pump ...................  256 11.1.3 Isocratic Pump.................. 258 11.2 Physical Specifications .................. 260 12 Accessories, Consumables and Replacement Parts .... 261 12.1 General Information ..................

  • Page 11: Using This Manual

    • Using this Manual 1 Using this Manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 11 Operating Manual...

  • Page 12: About This Manual

    • Using this Manual About this Manual This manual describes the functional features and operating principle of your Vanquish™ device and provides instructions for installation, set up, start up, shut down, operation, maintenance and troubleshooting. The layout of this manual is designed to provide quick reference to the sections of interest to the user.

  • Page 13: Conventions

    • Using this Manual Conventions This section describes the conventions that are used throughout this manual. 1.2.1 Safety Messages The safety messages and precautionary statements in this manual appear as follows: • Safety messages or precautionary statements that apply to the entire manual and all procedures in this manual are grouped in the Safety chapter.

  • Page 14: Typographical Conventions

    • Using this Manual 1.2.3 Typographical Conventions These typographical conventions apply to the descriptions in this manual: Data Input and Output • The following appears in bold type: Input that you enter by the keyboard or that you select with the ¨...

  • Page 15: Reference Documentation

    • Using this Manual Reference Documentation In addition to this operating manual, other documentation is available for reference. Hardware Documentation Additional hardware documentation includes the following: • Operating manuals for the other modules of the Vanquish system A printed version of the manual is shipped with the device. •...
  • Page 16 • Using this Manual TIP The Chromeleon Help and documents are included in the software shipment. Third-Party Documentation Refer also to the user documentation provided by the manufacturers of third-party components and materials, for example, Safety Data Sheets (SDSs). Page 16 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 17: Safety

    • Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the device. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 17 Operating Manual...

  • Page 18: Safety Symbols And Signal Words

    • Safety Safety Symbols and Signal Words 2.1.1 Safety Symbols and Signal Words in This Manual This manual contains safety messages to prevent injury of the persons using the device. The safety symbols and signal words in this manual include the following: Always be aware of the safety information.

  • Page 19: Safety Symbols On The Device

    • Safety 2.1.3 Safety Symbols on the Device The table lists the safety symbols that appear on the device or on labels affixed to the device. Follow the safety notices in this manual to prevent the risk of operator injury or damage to the device. Symbol Description Indicates a potential hazard.

  • Page 20: Intended Use

    • Safety Intended Use The device is intended to be part of the Vanquish system. The intended use of the Vanquish system is to analyze mixtures of compounds in sample solutions. The device is for use by qualified personnel and in laboratory environment only.

  • Page 21: Safety Precautions

    • Safety Safety Precautions 2.3.1 General Safety Information All users must observe the general safety information presented in this section and all specific safety messages and precautionary statements elsewhere in this manual during all phases of installation, operation, troubleshooting, maintenance, shutdown, and transport of the device. If the device is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the device could be impaired.

  • Page 22: Qualification Of The Personnel

    • Safety 2.3.2 Qualification of the Personnel Observe the information below on the proper qualification of the personnel installing and/or operating the device. Installation Only skilled personnel are permitted to install the device and to establish the electrical connections according to the appropriate regulations.

  • Page 23: Electrical Safety Precautions

    • Safety Protective Eyewear To prevent liquids from striking your eyes, put on appropriate protective eyewear, such as safety glasses with side shields. If there is a risk of splashing liquids, put on goggles. Gloves To protect you from harmful liquids and avoid personal injury during maintenance or service, put on appropriate protective gloves.

  • Page 24: General Residual Hazards

    • Safety 2.3.5 General Residual Hazards Pay attention to the following general residual hazards when working with the device: WARNING—Hazardous Substances Solvents, mobile phases, samples, and reagents might contain toxic, carcinogenic, mutagenic, infectious, or otherwise harmful substances. The handling of these substances can pose health and safety risks. •...

  • Page 25: In Case Of Emergency

    • Safety WARNING—Self-Ignition of Solvents Solvents with a self-ignition temperature below 150 °C might ignite when in contact with a hot surface (for example, due to leakage in the chromatography system). Avoid the use of these solvents. WARNING—Hazardous Vapors Mobile phases and samples might contain volatile or flammable solvents.

  • Page 26: Solvent And Additive Information

    • Safety Solvent and Additive Information 2.4.1 General Compatibility To protect optimal functionality of the Vanquish system, observe these recommendations on the use of solvents and additives: • The system must be used with reversed-phase (RP) compatible solvents and additives only. •...

  • Page 27: Allowed Ph Ranges

    • Safety 2.4.2 Allowed pH Ranges Allowed pH ranges (standard system configuration): System Allowed Remarks (Standard pH ranges Configuration) Vanquish Core 1-13 • pH values of 2 or less: The application time should be as short as possible. Flush the system Vanquish 2-12 thoroughly after these applications.

  • Page 28: Further Information

    • Safety 2.4.4 Further Information • For details about the materials that are used in the analytical flow path of the device, see the Specifications chapter in this manual. For information about the materials that are used in the flow path of the other modules in the Vanquish system, refer to the Specifications chapter in the Operating Manual for the modules.

  • Page 29: Compliance Information

    • Safety Compliance Information Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the device is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in this manual.
  • Page 30 • Safety Page 30 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 31: Device Overview

    • Device Overview 3 Device Overview This chapter introduces you to the device and the main components. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 31 Operating Manual...

  • Page 32: Pump Features

    • Device Overview Pump Features The pump comprises the following features: Main Features • Serial dual-piston pump with electronic compressibility compensation (SmartFlow™) for precise and virtually pulse-free flow • Floating piston design for compensation of small mechanical tolerances within the specification and thus enhanced robustness of the pump •...

  • Page 33: Operating Principle

    • Device Overview Operating Principle General Information The pump is a serial dual-piston pump with electronic compressibility compensation for precise and virtually pulse-free flow. The pump head comprises a working cylinder and an equilibration cylinder that are connected in series. Thus, the solvent passes both cylinders successively.

  • Page 34: Interior Components

    • Device Overview Interior Components The user-accessible components of the pump are located directly behind the front doors. For pump-specific pictures that provide an overview of the interior components, see Pump-Specific Information (} page 215). Page 34 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 35: Piston Seal Wash System

    • Device Overview Piston Seal Wash System To keep the pistons wet and rinse the piston seals, the pump has an active piston seal wash system (seal wash system). Piston seal washing prolongs the lifetime of the pistons and seals by preventing crystallization and particle deposits on the piston surfaces and seals, which may otherwise damage the pistons and seals.

  • Page 36: Purge/Sensor Unit

    • Device Overview Purge/Sensor Unit The purge/sensor unit comprises the purge valve and the system pressure sensor. Figure 2: Purge/sensor unit (example, here in binary pump) Description Isocratic pump, dual pump, quaternary pump: Capillary from the pump head Binary pump: Capillary from the right pump head Binary pump only: Capillary from the left pump head System pressure sensor Capillary mixer...

  • Page 37: Mixing System

    • Device Overview Mixing System The pump is shipped with a mixing system, comprising the following mixers: • Capillary mixer In the capillary mixer, the solvent streams delivered by the pump are combined and premixed. • Static mixer The static mixer improves the mixing quality. Mixing with capillary mixer and static mixer results in smoother baselines.

  • Page 38: Leak Detection

    • Device Overview Leak Detection Leaks are a potential safety issue. The leak sensor inside the device monitors the device for liquid leaks from the flow connections. The liquid is collected in the leak tray and guided to the drain port. From the drain port, the liquid is discharged to waste through the drain system of the Vanquish system.

  • Page 39: Operation

    Operating Manual. Details on control and operation of the device are available in the Chromeleon Help. TIP The device can be operated also with other data systems, such as Thermo Scientific Xcalibur . In this case, installation of additional software is required in addition to the data system software. For details, contact the Thermo Fisher Scientific sales organization.
  • Page 40 • Device Overview Page 40 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 41: Unpacking

    • Unpacking 4 Unpacking This chapter provides information for unpacking the device and informs you about the scope of delivery. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 41 Operating Manual...

  • Page 42: Unpacking

    • Unpacking Unpacking Damaged Packaging, Defective on Arrival Inspect the shipping container for signs of external damage and, after unpacking, inspect the device for any signs of mechanical damage that might have occurred during shipment. If you suspect that the device may have been damaged during shipment, immediately notify the incoming carrier and Thermo Fisher Scientific about the damage.
  • Page 43 • Unpacking Figure 3: Carrying handles on the device Component Carrying handles Attachment screw (one on each carrying handle) 4. Place the device on a stable surface. 5. If applicable: Remove any additional packing material. Leave any protective films attached to the surfaces of the device until it is properly positioned in the system stack.

  • Page 44: Scope Of Delivery

    • Unpacking Scope of Delivery The following items are included in the delivery: • Pump • Ship Kit • Operating manual • Power cord For information on contents of the ship kit or reordering parts, see Accessories, Consumables and Replacement Parts (} page 261).

  • Page 45: Installation

    • Installation 5 Installation This chapter specifies the requirements for the installation site and describes how to set up, install, and configure the device in the Vanquish system and in the chromatography software. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 45 Operating Manual...

  • Page 46: Safety Guidelines For Installation

    • Installation Safety Guidelines for Installation Pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...

  • Page 47: Installing The Device

    • Installation Installing the Device The Vanquish system is installed and set up by a Thermo Fisher Scientific service engineer, including all modules and options or parts shipped with them. The service engineer checks that the installation is correct and that the Vanquish system and modules operate as specified.
  • Page 48 • Installation NOTICE If the device is operated with another data system, refer to the documentation for the software that you are using and/or perform the qualification manually. The Instruments Installation Qualification Operating Instructions provide information about the parameters to be adapted and the required settings.

  • Page 49: Site Requirements

    • Installation Site Requirements The operating environment is important to ensure optimal performance of the device. This section provides important requirements for the installation site. Note the following: • Operate the device only under appropriate laboratory conditions. • The device is intended to be part of the Vanquish system. Observe the site requirements for the Vanquish system as stated in the Vanquish System Operating Manual.

  • Page 50: Power Cord

    • Installation 5.3.2 Power Cord The power cords are designed to match the wall socket requirements of the country in which they are used. The end of the power cords that plugs into the power socket on the device is identical for all power cords. The end of the power cords that plugs into the wall socket is different.

  • Page 51: Accessing The Interior Components

    • Installation Accessing the Interior Components To access the interior components in the device, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the device are located directly behind the doors. Figure 5: Opening the front doors Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 51...

  • Page 52: Setting Up The Hardware

    • Installation Setting Up the Hardware This section describes how to set up the hardware and provides information about the device connectors and cables. 5.5.1 System Arrangement The device is part of the Vanquish system. The system modules are typically arranged in a system stack, with the arrangement depending on the system configuration.

  • Page 53: Connecting The Device

    • Installation 5.5.2 Connecting the Device Device Connectors The following connectors are provided on the device: Figure 7: Electrical connectors and device connectors on the pump Description Rating plate, indicating the serial number, part number, module name, revision number (if any), line and fuse rating, and the manufacturer's address Main power switch (on/off control) Fuse holder...
  • Page 54 • Installation Description USB (Universal Serial Bus) port ("B" type connector) Allows connection to other modules in the Vanquish system or the computer on which the data management system is installed, such as the Chromeleon software TIP Thermo Fisher Scientific recommends using the USB ports only as described above.

  • Page 55: Connecting The Power Cord

    • Installation 5.5.3 Connecting the Power Cord NOTICE Condensation in a device can damage the electronics. • Before connecting the devices to the power line, be sure that no condensation is present in the devices. • If you suspect that condensation is present, allow the device to warm up to room temperature slowly.

  • Page 56: Setting Up The Flow Connections

    • Installation Setting Up the Flow Connections This section describes how to set up the flow connections to and from the device and additional flow connections, if required. 5.6.1 General Information and Guidelines The pump is shipped from the manufacturing site with the flow connections installed between the interior components.

  • Page 57: Guiding Capillaries And Tubing Through The System

    • Installation Follow these steps To set up the additional flow connections and complete the installation, follow these steps: 1. Connect the pump to the drain system (see Guiding Liquids to Waste (} page 64)). 2. Connect the solvent lines (see Connecting the Solvent Lines (} page 64)).
  • Page 58 • Installation Use for Solvent tubing (up to three solvent lines) Solvent tubing (up to three solvent lines) Wash liquid tubing (seal wash, autosampler needle wash) Detector waste line Tubing brackets are available for holding the tubing in place. Slip the bracket side onto the drain pipe.

  • Page 59: Connecting Fittings, Capillaries, And Tubing

    • Installation 5.6.3 Connecting Fittings, Capillaries, and Tubing This section provides information about how to connect and handle capillaries, fittings, and tubing. 5.6.3.1 General Guidelines When connecting capillaries and tubing, follow these general recommendations: • Use only the capillaries and tubing (for example, solvent lines or waste tubing) that are shipped with the product or additional or spare capillaries and tubing as recommended by Thermo Fisher Scientific.
  • Page 60 • Installation Figure 10: Viper fittings (examples) Description Viper fitting, torque toothing Viper fitting, knurl When tightening or loosening a Viper capillary, observe the handling instructions for the respective fitting further down. Connecting Viper Fittings with Torque Toothing To connect Viper fittings with torque toothing, follow these steps: NOTICE •...
  • Page 61 • Installation 1. Insert the Viper capillary into the connection port. 2. Tighten the connection with your fingers. 3. Use the installation tool to tighten the connection further. Figure 12: Tool on torque toothing for tightening connections 4. Tighten the connection until the torque limitation has been reached, which is indicated by a clicking sound.
  • Page 62 • Installation Figure 14: Viper fitting with knurl Description Knurl Capillary Slot 1. Insert the Viper capillary into the connection port. 2. Tighten the connection by the knurl. TIP Note the slot in the knurl. For narrow connections, you can easily remove the knurls from neighboring capillaries through this slot and attach them again later.
  • Page 63 • Installation 5.6.3.3 Connecting Non-Viper Capillaries To connect capillaries with conventional fitting connections (using ferrules) (non-Viper), follow these steps: 1. Insert the capillary into the connection port. 2. Tighten the connection with a wrench (wrench size: 1/4"). NOTICE Overtightening may damage the connection. With a torque wrench, the torque should not exceed 3 Nm (for steel capillaries).

  • Page 64: Guiding Liquids To Waste

    • Installation 5.6.4 Guiding Liquids to Waste The following liquids collect in the leak tray and flow off through the drain port at the bottom right of the leak tray into the drain system of the Vanquish system: • Leaking liquid from the leak tray •...
  • Page 65 • Installation Pump Type Pump Inlet Isocratic pump The pump inlet is the pump head. Quaternary pump, The pump inlet is the vacuum degasser. dual pump For the pump-specific operating principles that illustrate the solvent flow through the pump, see Pump-Specific Information (} page 215).
  • Page 66 • Installation 1. Assemble the solvent line filter, wearing appropriate clean gloves: a) Place the frit in the filter holder (bottom part). b) Make sure that the frit is in a level position. c) Screw the filter top to the filter bottom. 2.
  • Page 67 • Installation TIP To avoid the formation of gas bubbles in the solvent reservoirs, place the reservoirs in the system stack on the same level or higher than the pump. The setup description below assumes that the reservoirs are placed in the solvent rack. Routing the Solvent Lines to the Solvent Rack 1.
  • Page 68 • Installation Connecting the Solvent Line to the Solvent Reservoir Figure 19: Preparing the solvent line and reservoir cap Description Solvent line Retaining guide Reservoir cap Cap plugs Solvent line filter 1. Feed each solvent line through a retaining guide. 2. Feed the solvent line through an open hole in a cap for a solvent reservoir.

  • Page 69: Installing Solvent Shut Off Valves

    • Installation 9. Check the solvent lines across the entire flow path. Make sure that the lines are not bent, pinched, or squeezed at any point in the flow path. 10. If applicable, reinstall the seal wash detector assembly. 5.6.6 Installing Solvent Shut Off Valves These steps apply only when installing a shut off valve during initial setup of the pump.
  • Page 70 • Installation 1. Cut the solvent line near the reservoir cap with a tubing cutter. Make sure that the cut is at a right angle to the length of the line. 2. Onto the free end of each solvent line, slide a fitting and a ferrule. Mind the correct orientation of the fitting and ferrule (see picture).

  • Page 71: Seal Wash System

    • Installation Seal Wash System The flow path of the seal wash system passes through the metering device head in the autosampler and the pump heads in the pump. The seal wash system comprises the following parts: • In the pump: Seal wash pump (peristaltic pump), seal wash lines, and seal wash detector (drop detector) •...

  • Page 72: Setting Up The Seal Wash System

    • Installation 5.7.2 Setting Up the Seal Wash System Parts and tools required • Seal wash reservoir assembly, including reservoir, reservoir cap, cap plugs, and retaining guide for the seal wash line • Seal wash line (silicone tubing) for connection to the seal wash pump;...
  • Page 73 • Installation Figure 22: Seal wash system in the Vanquish system (examples with a single split sampler, left: with quaternary pump, right: with binary pump, as example for two-head pump) Description Seal wash reservoir Seal wash lines in the autosampler (thick tubing), with Wash line from reservoir to inlet port Seal wash inlet port (right tubing connector) Wash line from inlet port to metering device head...
  • Page 74 • Installation Description Isocratic pump, quaternary pump: Wash line from pump head to seal wash detector Binary pump, dual pump: Wash line from left pump head to right pump head Binary pump, dual pump: Wash line from right pump head to seal wash detector Seal wash detector (drop detector) Follow these steps...
  • Page 75 • Installation Description Peristaltic tubing Seal wash tubing to pump head 1. Press down the lever on the seal wash pump and hold it in that position with one hand. 2. With your other hand, insert the tubing between the lever and the rotor and wind it around the rotor.
  • Page 76 • Installation Figure 24: Seal wash connections Description Single split sampler: Seal wash connections on the top enclosure frame Dual split sampler: Seal wash connections on the right enclosure frame Seal wash inlet port Seal wash line to reservoir Seal wash outlet Seal wash line to pump 4.
  • Page 77 • Installation 4. Route the seal wash line through the guide hole in the solvent rack. Figure 25: Guide hole and tubing guides in the solvent rack Description Guide hole (solvent rack) Not to be used for wash liquid line; reserved for other tubing Tubing guide for seal wash line Not to be used for wash liquid line;...

  • Page 78: Flushing The Seal Wash System

    • Installation 4. Fill the seal wash reservoir with seal wash liquid. Mind the requirements outlined in the previous section. Before using a seal wash reservoir for the first time, rinse the reservoir thoroughly. Use a high-purity solvent for rinsing. 5.
  • Page 79 • Installation Flushing the Seal Wash System from Hand To flush the seal wash system from hand, follow these steps: 1. Disconnect the tubing from the seal wash detector. Figure 27: Seal wash detector Description Seal wash detector Tubing connection port (port labeled in) 2.

  • Page 80: Connecting The Pump And Autosampler

    • Installation Connecting the Pump and Autosampler Parts required Capillary to connect the pump to the autosampler, as required for the flow path Follow these steps 1. On the connecting capillary, remove the knurls. 2. Guide the connecting capillary through the guide holes in the housings of both, the pump and autosampler.

  • Page 81: Turning On The Device

    • Installation Turning On the Device Before turning on the power to a Vanquish system module for the first time, verify that the chromatography software is installed on the data system computer. When the power is turned on, the required USB drivers are automatically found and the Windows™...

  • Page 82: Setting Up The Device In The Software

    • Installation 5.10 Setting Up the Device in the Software This manual assumes that the chromatography software is already installed on the data system computer and a valid license is available. For more information about setting up the Vanquish system in the software, refer to the Vanquish System Operating Manual.

  • Page 83: Operation

    • Operation 6 Operation This chapter describes the elements for device control, provides information for routine operation and for shutdown. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 83 Operating Manual...

  • Page 84: Introduction To This Chapter

    • Operation Introduction to this Chapter The information in this chapter assumes that the initial setup of the device has already been completed. If this is not the case, refer to the instructions in Installation (} page 45). For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.

  • Page 85: Safety Guidelines For Operation

    • Operation Safety Guidelines for Operation When operating the device, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). CAUTION—High Luminosity of LED The high luminosity produced by the LED illuminating the inside of the device can be harmful to the eyes.

  • Page 86: Control Elements

    • Operation Control Elements The device is designed to be operated mainly from a computer running with the chromatography software. In addition, the following elements are available on the device: • Keypad The keypad buttons allow you to perform certain functions directly from the device.
  • Page 87 • Operation MUTE ALARM Beeping alerts you when the device detects a problem, for example, leakage. To turn off the beep for the current alarm, press this button. Eliminate the source for the alarm within 10 minutes. Otherwise, beeping starts again. If the device detects a different problem, beeping alerts you again immediately.

  • Page 88: Status Indicators

    • Operation PURGE Pressing the button starts a purge cycle. Pressing the button while a purge cycle is running stops the cycle. For more information about purging, see Purging the Pump (} page 103). FLOW Pressing the button starts or stops the flow, considering the selected flow rate, solvent composition, and flow acceleration/deceleration.
  • Page 89 • Operation TIP—Dual pump only: With a dual pump, the LED bar is a two-part bar, with the lights on the left referring to the left pump and the lights on the right referring to the right pump. LED Bar Description Off (dark) The power to the device is turned off.
  • Page 90 • Operation Operational Status Green, flashing – during In addition, the message "Compression limit normal operation reached" (code 4156) appears in the Chromeleon Audit Trail. The compression value was 100% during each of the past three strokes. If the compression value is lower than 100% for several strokes afterward, the message "Compression back to normal"(code 4157) will appear in the Audit Trail.

  • Page 91: Power On/Off Control

    • Operation Power On/Off Control The power switch on the device is the main switch for power on/off control. The main power switch is turned on during initial installation of the device. For easier handling, you can use the power button on the front left of the Vanquish system base (system power button) for power on/off.

  • Page 92: Use Of Solvents And Additives

    • Operation Use of Solvents and Additives Particles entering the chromatography system can block capillaries and valves, increase wear, and damage the column or system. Especially with aqueous solvents, algae and other microorganisms can grow and deposit in the chromatography system and block the solvent line filters. Blocked capillaries or filters can cause increased or unstable system pressure.
  • Page 93 • Operation • The pumps are shipped with the following piston seals: Pump Piston Seals VC‑pumps except The pumps are shipped with UHMW-PE piston seals. Using VC‑P21 and VC‑P33 tetrahydrofuran, ketones, or ammonium hydroxide as solvents can damage the seals. Under harsh conditions, consider replacing the UHMW-PE piston seals with carbon-fiber filled PTFE piston seals (part no.
  • Page 94 • Operation TIP In a Vanquish Core system, normal-phase (NP) compatible solvents and additives may be used if the VC-pumps and the VC-autosamplers are modified with the components from the Normal-Phase (NP) kit. See Normal-Phase Compatible Solvents and Additives (} page 249). Page 94 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 95: Preparing The Device For Operation

    • Operation Preparing the Device for Operation This section gives information on any additional steps that are required to prepare the device for operation and sample analysis. Before Operating the Device for the First Time Prepare the device for the first-time operation, observing the following: NOTICE Flush the system flow path thoroughly before operating the device for the first time:...
  • Page 96 • Operation System Equilibration System equilibration should include the following operations: • Purging the pump (all channels, including those not used for the application) • Flushing the entire chromatography system with the starting solvent to rinse out any solvent from a previous analysis run •...

  • Page 97: Important Operating Parameters

    • Operation Important Operating Parameters The parameters described in this section should be considered for routine operation of the device. You can usually access these parameters from the Chromeleon user interface. If a parameter listed below is not available in the Chromeleon software, consider updating the firmware and Chromeleon version.
  • Page 98 • Operation Parameter Description Pump pressure In the dialog box for the pump in the Instrument Configuration Manager, the Pump_Pressure check box is selected as a standard when the pump is set up in the Chromeleon software. The Chromeleon software uses this setting to generate the channel for recording the pump pressure (which corresponds to the column pressure).
  • Page 99 • Operation Solvent and Waste Liquid Parameters Parameter Description Solvent composition In the dialog box for the pump in the Instrument Configuration Manager, set the number of the solvents to be used with the pump. You can change the standard solvent names as required. The solvent names appear in the Chromeleon user interface.
  • Page 100 • Operation Parameter Description Waste level You can monitor the liquid level in the waste container when you have entered the required information. Based on this information, the Chromeleon software calculates the liquid level. To monitor the liquid level in real time, based on real physical measurement, install the Vanquish Solvent Monitor.
  • Page 101 • Operation Other Parameters Parameter Description Compression The compression values of the pump head can provide valuable information for troubleshooting. For details, see Checking the Compression Values (} page 210). Curve You can specify linear or non-linear (curved) gradient profiles. • Curve 5 (default setting) is linear. Changes in the composition of the delivered solvent over time are constant.
  • Page 102 • Operation Parameter Description Seal washing The seal wash system is enabled and cannot be disabled. As a standard, the pump performs a seal wash in the following situations: • Once per hour • Each time the pump is turned on If required, you can start an additional seal wash cycle or stop a running cycle: •...

  • Page 103: Purging The Pump

    • Operation Purging the Pump Purging the pump means rinsing the pump for a short time at a higher flow rate, using the selected solvents. Settings The following settings are considered for the purge cycle: Setting Description Purge flow The standard setting is 5 mL/min. You can adapt the setting in the Chromeleon software if required.

  • Page 104: Automatic Purging

    • Operation 6.8.1 Automatic Purging Automatic purging means that the solvent is automatically directed to waste, through the wash port of the Vanquish autosampler, without further user interaction. Prerequisites Automatic purging is possible only when the pump is fluidically connected to the Vanquish autosampler and when both modules have an appropriate firmware installed.

  • Page 105: Manual Purging

    • Operation Follow these steps 1. In the Chromeleon software, set the channel that you want to purge to 100%. 2. Optional: In the Chromeleon software, adapt the settings for the purge flow or purge time if required. 3. Start the purge cycle. You can start the purge cycle from the Chromeleon software or by pressing the PURGE button on the keypad.
  • Page 106 • Operation Items required • Solvents suitable for purging To flush out the isopropanol when operating the pump for the first time, use solvents that are miscible with isopropanol. If they are not, use an appropriate intermediate solvent. • Silicone tubing •...
  • Page 107 • Operation 7. Remove the syringe from the silicone tubing, absorbing any liquid that leaves the tubing with a tissue. 8. Route the free end of the tubing to the drain port outlet (see Guiding Liquids to Waste (} page 64)). 9. Perform the above steps for all channels, including those that are not used for the application.
  • Page 108 • Operation 6.8.2.2 Purging for Removing Air Bubbles or Changing to Different Solvent Type • To remove air bubbles that might be trapped in the system (pump head, solvent lines) because air bubbles in the system may lead to the following problems: Pressure pulsation ¨...
  • Page 109 • Operation 4. Start the purge cycle. You can start the purge cycle from the Chromeleon software or by pressing the PURGE button on the keypad. 5. Perform the above steps for all channels, including those that are not used for the application. 6.

  • Page 110: Optimizing The Performance Of The Device

    • Operation Optimizing the Performance of the Device This section provides information for best performance of the device and gives hints on what you can do to optimize the performance further. 6.9.1 General Guidelines To optimize the performance of the device, consider the following general guidelines: •...
  • Page 111 • Operation Parts and additional items required • Shut off valve, kit containing one shut off valve and fitting connections • Tubing cutter • Solvent suitable for purging the pump Preparations 1. Turn off the pump flow and wait until the system pressure is down to zero.
  • Page 112 • Operation 5. Cut the solvent line near the reservoir cap with a tubing cutter. Make sure that the cut is at a right angle to the length of the line. 6. Onto the free end of each solvent line, slide a fitting and a ferrule. Mind the correct orientation of the fitting and ferrule (see picture).

  • Page 113: Shutting Down The Device

    • Operation 6.10 Shutting Down the Device If the device will not be operated for some time, follow the instructions in this section to shut down the device. TIP The Chromeleon software provides procedures for automatically preparing the chromatography system for shutdown. The procedures include, for example, operations for reducing the flow rate, reducing the temperature in temperature-controlled devices, and turning off the detector lamps.

  • Page 114: Long-Term Shutdown

    • Operation 6.10.2 Long-Term Shutdown To interrupt operation for a longer period, follow the instructions in this section. TIP Shutting down the device affects the operation of the system. When shutting down the device, also observe the shutting down instructions for the other Vanquish system modules and take appropriate action (refer to the Operating Manuals for the modules).
  • Page 115 • Operation 3. Turn off the pump flow and wait until the system pressure is down to zero. 4. Disconnect the solvent lines. See Disconnecting the Solvent Lines (} page 115). TIP Removing the solvent lines from the tubing guides is usually not required (not even for replacing the slide-in module).

  • Page 116: Restart After Long-Term Shutdown

    • Operation Emptying the Seal Wash System 1. Unscrew the cap of the seal wash reservoir. 2. Together with the cap, remove the seal wash line from the seal wash reservoir. 3. Remove the peristaltic tubing from the seal wash pump. When the tubing is removed, any liquid that may be present in the seal wash line, flows off to waste.

  • Page 117: Maintenance And Service

    • Maintenance and Service 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 117 Operating Manual...

  • Page 118: Introduction To Maintenance And Service

    • Maintenance and Service Introduction to Maintenance and Service This chapter describes the routine maintenance and service and repair procedures that the user may perform. Additional maintenance or service procedures must be performed only by service personnel certified by Thermo Fisher Scientific (for brevity, referred to as Thermo Fisher Scientific service personnel).

  • Page 119: Safety Guidelines For Maintenance And Service

    • Maintenance and Service Safety Guidelines for Maintenance and Service When performing maintenance or service procedures, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented Safety Precautions (} page 21). WARNING—High Voltage High voltages are present inside the device that could cause an electric shock.
  • Page 120 • Maintenance and Service CAUTION—Hydrostatic Pressure Solvent may spill when you open the flow path. This is due to hydrostatic pressure in the system when the solvent reservoirs are located above the pump outlet. Before you loosen a connection in the flow path: •...

  • Page 121: General Rules For Maintenance And Service

    • Maintenance and Service General Rules for Maintenance and Service For successful maintenance and service procedures, follow these rules and recommendations: • Before starting maintenance or service procedures, shut down the device when instructed to do so. • Use only the replacement parts specifically authorized and qualified for the device by Thermo Fisher Scientific.

  • Page 122: Routine And Preventive Maintenance

    • Maintenance and Service Routine and Preventive Maintenance Optimum device performance, maximum uptime of the device, and accurate results can be obtained only if the device is in good condition and properly maintained. 7.4.1 Internal Maintenance Every six weeks, the pump automatically performs an internal maintenance procedure when you initiate a purge cycle.
  • Page 123 • Maintenance and Service Frequency What you should do... Regularly • Replace the wash liquid in the seal wash reservoir with fresh liquid. Rinse the reservoir thoroughly before filling it. Use a high-purity solvent for rinsing. • Test the seal wash system for leakage (see Testing the Seal Wash System for Leakage (} page 130)).

  • Page 124: Flushing The Pump

    • Maintenance and Service 7.4.3 Flushing the Pump When • To avoid contamination of the pump • With persistent contamination 7.4.3.1 Flushing to Avoid Contamination When To avoid contamination of the degassing channels Items required Fresh solvent Observe the following guidelines The following guidelines refer to conventional use of the Vanquish system as recommended in this manual, such as using fresh high-quality solvents (minimum UHPLC-grade or LC/MS-grade) that are replaced...
  • Page 125 • Maintenance and Service 7.4.3.2 Flushing with Persistent Contamination Only When TIP With conventional use of the Vanquish system as recommended in this manual, regularly flushing the degassing channels is usually sufficient (see Flushing to Avoid Contamination (} page 124)). With persistent contamination, for example, if ghost peaks appear reproducibly in your blank chromatogram without injecting a sample, consider a harsher cleaning procedure.

  • Page 126: Cleaning Or Decontaminating The Device

    • Maintenance and Service 10. Turn off the pump flow and wait until the system pressure is down to zero. 11. Uninstall the backpressure capillary and reconnect the system as required by your application. 12. Purge the pump. 13. Equilibrate the system. 7.4.4 Cleaning or Decontaminating the Device Cleaning and decontamination must be performed by qualified...

  • Page 127: Predictive Performance

    • Maintenance and Service NOTICE Observe the following: • Only use cleaning detergents that will not damage the surfaces of the system. • Never use sharp tools or brushes for cleaning any surfaces. • Do not use sprays for cleaning. •...
  • Page 128 • Maintenance and Service Color-coded bars on special panels provide visual feedback, allowing you to easily check and monitor the status. If a warning limit was set, a message in the Chromeleon Audit Trail alerts you when the action is due.

  • Page 129: Seal Wash System

    • Maintenance and Service Seal Wash System Follow the sequence of steps for the maintenance procedure that you want to perform: • To test the seal wash system for leakage, follow the steps in Testing the Seal Wash System for Leakage (} page 130).

  • Page 130: Testing The Seal Wash System For Leakage

    • Maintenance and Service 7.5.1 Testing the Seal Wash System for Leakage When • At regular intervals • If you performed maintenance on components in the pump head, for example, after replacing the piston seals Items required • Syringe (12 mL) •...

  • Page 131: Replacing The Seal Wash Lines

    • Maintenance and Service 7. Check whether liquid escapes from the connections of the seal wash system or under the pump heads. Observation Remedial Action Leakage is present Tighten or replace leaky connections. Repeat the inspection. No leakage is present Remove the syringe and reconnect the tubing to the seal wash detector (port labeled in).
  • Page 132 • Maintenance and Service Follow these steps NOTICE When you must remove a seal wash line from the tubing guides, be careful not to pull on other tubing in the guides. Follow the sequence of steps for the seal wash line that you want to replace: •...
  • Page 133 • Maintenance and Service 1. Disconnect the peristaltic tubing from the silicone tubing. 2. Press down the lever on the seal wash pump and remove the peristaltic tubing from the pump and from the tubing holders. 3. Connect the new peristaltic tubing to the silicone tubing. 4.

  • Page 134: Replacing The Seal Wash Detector

    • Maintenance and Service 4. Check the wash lines over the entire flow path: a) Make sure that no bending (kink), pinching or squeezing of the seal wash lines is present at any point in the flow path. b) If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
  • Page 135 • Maintenance and Service 3. Grasp and pull the seal wash detector assembly upward by the funnel. 4. Push the new seal wash detector assembly into the pump. The seal wash detector and funnel come as an assembled unit. If you have to assemble both parts for any reason, push the detector into the funnel first.

  • Page 136: Pump Head

    • Maintenance and Service Pump Head Follow the sequence of steps for the maintenance procedure that you want to perform: NOTICE All pumps except VC-P21 and VC-P33: Whenever you remove the pump head, you must replace the seal wash seals. •...

  • Page 137: Overview Of Pump Head Parts

    • Maintenance and Service 7.6.1 Overview of Pump Head Parts The picture shows the main pump head parts. Figure 36: Overview of pump head parts Description Inlet check valve, including: Valve nut Valve cartridge Pump head Pump head seals (2 PTFE O-ring seals) Piston seals Support rings Seal wash bodies (pump head bushing)

  • Page 138: Replacing The Pump Head

    • Maintenance and Service 7.6.2 Replacing the Pump Head When • Damage of pump head • Problems with pump performance Parts required • Pump head • Spacing tool (pump head tool) Tools and additional items required • Hexagon wrench, size 6 •...
  • Page 139 • Maintenance and Service 7.6.2.1 Removing the Pump Head Preparations Prepare the pump for removing the pump head. See Replacing the Pump Head (} page 138). Follow these steps Figure 37: Pump head Description Pump head inlet Pump head screw Pump head outlet Seal wash tubing 1.
  • Page 140 • Maintenance and Service 5. On the pump head, disconnect the following tubing and capillaries: On the pump head inlet, disconnect the tubing. ¨ Remove the seal wash lines from the tubing connectors on the ¨ pump head. Do not remove the tubing connectors. 6.
  • Page 141 • Maintenance and Service • Dual pump: To identify the correct installation position, note the labeling on the U-tube (capillary from working cylinder to equilibration cylinder) (L for the pump head of the left pump or R for the pump head of the right pump). Installation of new pump heads (replacement pump heads) •...
  • Page 142 • Maintenance and Service 4. Binary pump: Connect the cable of the pressure sensor to the P- WORK connector. 5. Dock the pistons either from the keypad or from the Chromeleon software. Wait until docking is complete before proceeding with the next step.
  • Page 143 • Maintenance and Service 7.6.2.3 Flushing the Seal Wash System When To flush the seal wash system during pump head installation, follow the steps further down. To flush the seal wash system for any other reason, see Flushing the Seal Wash System (} page 78).

  • Page 144: Replacing The Pistons

    • Maintenance and Service 7.6.3 Replacing the Pistons When Damage of piston Parts and additional items required • Pistons • Syringe filled with isopropanol • Seal wash seals (and seal-handling tool) • Seal wash liquid • Solvent suitable for your application NOTICE All pumps except VC-P21 and VC-P33: Whenever you remove the pump head, you must replace the seal wash seals.
  • Page 145 • Maintenance and Service Figure 41: Installing the spacing tool and pistons Description Pistons Spacing tool 6. Push in the pistons until they contact the tool. 7. Remove the spacing tool. Be careful not to pull out the pistons any further. 8.

  • Page 146: Cleaning The Piston

    • Maintenance and Service 7.6.4 Cleaning the Piston When When reinstalling the piston that you removed Items required • Dry, lint-free tissue • Light source • Isopropanol Follow these steps 1. Inspect the piston for signs auf damage. TIP Hold the rear side of the piston into the light. The refraction of the light makes dirt particles appear larger.

  • Page 147: Replacing The Piston Seals Or Support Ring

    • Maintenance and Service 7.6.5 Replacing the Piston Seals or Support Ring When • Damage to piston seals, leaky seals • Unstable flow rates or baseline noise Parts required • Piston seals • Support ring Note that the support ring is not a wear part. You need not replace the support ring when you replace a piston seal.
  • Page 148 • Maintenance and Service Preparations 1. Remove the pump head (see Replacing the Pump Head (} page 138)). 2. Remove the pistons (see Replacing the Pistons (} page 144)). Follow these steps 1. Remove the seal wash plate from the pump head if applicable. Figure 43: Pump head, seal wash plate removed Description Pistons...
  • Page 149 • Maintenance and Service 4. Remove the piston seal. With the removal pin, push the seal-handling tool into the pump head as far as it goes in and remove the tool. The piston seal is removed together with the tool. Once the seal has been removed, it cannot be reused.
  • Page 150 • Maintenance and Service Description Support ring Piston seal (open side facing away from the tool) 9. With the insertion pin, push the seal-handling tool into the pump head as far as it goes in. 10. Remove the tool from the pump head. The piston seal and support ring remain in the pump head.

  • Page 151: Recommendations For New Piston Seals

    • Maintenance and Service 16. Inspect all flow connections on the pump head for indications of leakage. Tighten leaky connections when the system pressure is down to zero. 17. In the Chromeleon software, consider updating the Predictive Performance information for the piston seals. 18.
  • Page 152 • Maintenance and Service Allowing New Piston Seals to Run In 1. On the purge/sensor unit, attach silicone tubing to the purge outlet (if it is not yet connected). 2. Route the free end of the tubing to the drain port outlet (see Guiding Liquids to Waste (} page 64)).
  • Page 153 • Maintenance and Service Emptying the Silicone Tubing NOTICE Always empty the silicone tubing when purging is complete. When the purge valve is closed, purge liquid remains in the tubing connected to the purge outlet. Leaving the liquid in the tubing for a longer period limits the tubing lifetime.

  • Page 154: Replacing The Seal Wash Seals

    • Maintenance and Service 7.6.7 Replacing the Seal Wash Seals When • Damage to the seal wash seal, leaky seal • Whenever you removed the pump head • Once a year Parts required Seal wash seals Tools and additional items required •...
  • Page 155 • Maintenance and Service Follow these steps 1. Remove the seal wash plate from the pump head if applicable. Figure 49: Pump head, seal wash plate, and pistons removed Description Pump head Seal wash plate, channel side Seal wash seals Pistons 2.
  • Page 156 • Maintenance and Service Figure 51: Seal wash plate (no-channel side) Description Seal wash plate (no-channel side) Seal wash seals 7. Place the seal wash plate onto the pump head, with the seals facing the pump head. Figure 52: Pump head with seal wash plate installed 8.

  • Page 157: Replacing The Pump Head Seals

    • Maintenance and Service Specific Steps After Piston Seal Replacement If you replaced the piston seals together with the seal wash seals, proceed with these steps: 1. Observe the recommendations for new piston seals (see Recommendations for New Piston Seals (} page 151)).
  • Page 158 • Maintenance and Service Figure 53: Pump head seals Description Seal wash bodies Pump head seal, O-ring 32x1.5 Pump head seal, O-ring 9x1.5 4. Install the new seals. Place the seal onto the receiving groove. With your finger on the seal, follow the groove shape until the seal rests exactly in the groove.

  • Page 159: Testing The Piston Seals For Leakage

    • Maintenance and Service 9. Inspect all flow connections on the pump head for indications of leakage. Tighten leaky connections when the system pressure is down to zero. 10. Optional: Test the system wash system and pump for leakage (see Testing the Seal Wash System for Leakage (} page 130) Testing the Pump for Leakage (General Test)
  • Page 160 • Maintenance and Service 3. Remove some liquid from the tubing, for example, by shaking the tubing. 4. Set up the system in such a way that the backpressure indicated in the table is produced. For example, install an appropriate backpressure capillary on the pump outlet (outlet of the static mixer).
  • Page 161 • Maintenance and Service 10. For about 5 minutes, observe the liquid level in the tubing. Finish the observation before a new seal wash cycle starts. 11. Depends on your observation: Observation Remedial Action Liquid level in the tubing is 1.

  • Page 162: Check Valves

    • Maintenance and Service Check Valves Follow the sequence of steps for the maintenance procedure that you want to perform: • To replace the inlet check valves, see the next section. • To replace the outlet check valves, see Replacing the Outlet Check Valves (} page 164).
  • Page 163 • Maintenance and Service When Damage of check valve or leaky check valve Parts required • Valve cartridge • Valve nut, required only when leakage is present at the fitting connection to the valve Tools and additional items required • Solvent suitable for purging to flush out harmful substances •...

  • Page 164: Replacing The Outlet Check Valves

    • Maintenance and Service NOTICE Overtightening may damage the valve cartridge. The torque should not exceed 10 Nm. 6. On the valve nut, reconnect the tubing. 7. Rinse the pump thoroughly: Use the solvent for your application. ¨ Open the purge valve to prevent the rinsing liquid from entering ¨...
  • Page 165 • Maintenance and Service When Damage of check valve or leaky check valve Parts required • Valve cartridge • Valve nut, required only when leakage is present at the fitting connection to the valve Tools and additional items required • Solvent suitable for purging to flush out harmful substances •...
  • Page 166 • Maintenance and Service 5. Insert the new cartridge into the valve nut. Insert the cartridge in the direction of flow (indicated by the arrow on the cartridge). 6. Screw the valve nut into the pump head and tighten with the open- end wrench.

  • Page 167: Cleaning The Check Valves

    • Maintenance and Service 7.7.3 Cleaning the Check Valves When • Dirty check valves • Once a year Parts and additional items required • Syringe (12 mL), filled with isopropanol • Ultrasonic bath Preparations Remove the check valves that you want to clean. It is recommended to clean all check valves of the pump head at the same time.

  • Page 168: Mixing System

    • Maintenance and Service Mixing System TIP The information and references to the static mixer in this section apply equally to the inline filter if installed. Follow the sequence of steps for the maintenance procedure that you want to perform: •...

  • Page 169: Replacing The Static Mixer Or Capillary Mixer

    • Maintenance and Service 5. Depends on the pressure reading: Pressure Reading Action Pressure is less than 0.8 MPa Continue with the next step. Pressure is 0.8 MPa or higher Replace the static mixer. 6. Reconnect the capillary to the outlet of the static mixer. 7.
  • Page 170 • Maintenance and Service Follow these steps NOTICE Turning the static mixer in the mounting bracket when loosening or tightening capillaries can damage the mounting bracket. To prevent the mixer from turning in the bracket, hold the mixer with an open-end wrench when you loosen or tighten capillaries on the mixer.

  • Page 171: Solvent Lines And Solvent Line Filters

    • Maintenance and Service Solvent Lines and Solvent Line Filters Follow the sequence of steps for the maintenance procedure that you want to perform: • To empty the solvent lines, see Emptying the Solvent Lines (} page 171). • To replace solvent lines, see Replacing Solvent Lines (} page 172).

  • Page 172: Replacing Solvent Lines

    • Maintenance and Service 7.9.2 Replacing Solvent Lines To replace solvent lines, follow the sequence of steps for the solvent line that you want to replace: The flow path determines the pump inlet (component at which the solvent enters the pump) and the solvent flow through the pump. Pump Type Pump Inlet Binary pump...
  • Page 173 • Maintenance and Service 7.9.2.1 Solvent Lines From Reservoir To Pump Inlet When Damage or blockage of the solvent line Parts and additional items required • Solvent line between solvent reservoir and pump inlet • Solvent suitable for purging Preparations •...
  • Page 174 • Maintenance and Service Preparations • Empty the solvent lines (see Emptying the Solvent Lines (} page 171)). Follow these steps 1. Retighten the reservoir cap hand-tight. Verify that the retaining guide remains in the hole in the reservoir cap. If it does not, press the retaining guide into the hole to ensure that the tubing is kept in place in the cap.

  • Page 175: Replacing The Solvent Line Filter

    • Maintenance and Service 7.9.3 Replacing the Solvent Line Filter When • Damage of solvent line filter • Impaired permeability of filter frit Parts and additional items required Figure 58: Solvent line filter Description Solvent line filter, filter holder bottom part Filter frit Solvent line filter, filter holder top part •...
  • Page 176 • Maintenance and Service 4. Assemble the solvent line filter, wearing appropriate clean gloves: a) Place the frit in the filter holder (bottom part). b) Make sure that the frit is in a level position. c) Screw the filter top to the filter bottom. 5.

  • Page 177: Replacing The Purge Valve Knob

    • Maintenance and Service 7.10 Replacing the Purge Valve Knob When • Leakage is observed around the valve knob when the valve is open. • Leakage is observed on the purge outlet when the valve is closed. Parts required Purge valve knob (with integrated seals) Figure 59: Purge valve knob with integrated seals Description Purge valve knob...
  • Page 178 • Maintenance and Service Follow these steps 1. To remove the purge valve knob from the purge/sensor unit, turn the valve knob counterclockwise all the way and pull it straight off the purge/sensor unit. 2. Before installing the new purge valve knob, clean the opening in the purge/sensor unit with a cleaning swab.

  • Page 179: Testing The Pump For Leakage (General Test)

    • Maintenance and Service 7.11 Testing the Pump for Leakage (General Test) When After maintenance on the fluidic system of the pump, for example on the flow connections, pump head, or check valves TIP With the dual pump, test each pump separately. Parts and additional items required •...
  • Page 180 • Maintenance and Service Pump Flow VC‑P21, VC‑P33 4 µL/min VF‑pump 1 µL/min 8. Observe the pressure. The pressure should increase or remain constant for a minimum of 1 minute. A drop in pressure indicates possible leakage. 9. Find and eliminate the cause for the leak. Take remedial action only when the system pressure is down to zero.
  • Page 181 • Maintenance and Service 5. Monitor if the pressure drops at the same rate as before. If the pressure drops significantly slower, the connection was leaky. 6. When testing the pump for leakage is complete, reset the upper pressure limit to the value used before the leakage test. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 181 Operating Manual...

  • Page 182: Replacing The Main Power Fuses

    • Maintenance and Service 7.12 Replacing the Main Power Fuses When Blown fuses Parts required Fuses (2 fuses, 3.15 AT, 250 V AC, slow-blow, 5 x 20 mm) from Fuses Kit Tools required Slotted screwdriver, any size between 3.3 mm and 5.5 mm is appropriate Preparations WARNING—Electric Shock High voltages are present inside the device that could cause an electric...
  • Page 183 • Maintenance and Service 1. Use the screwdriver to remove the fuse holder. 2. Replace the two fuses with new fuses of the specified type and current rating. Always replace both fuses. 3. Reinstall the fuse holder. 4. Reconnect the power cord to the power source and to the device. 5.

  • Page 184: Updating The Device Firmware

    • Maintenance and Service 7.13 Updating the Device Firmware When Updating the device firmware might be required, for example, when a new firmware version is released that adds functionality or solves problems of a previous version. Items required Firmware version/Chromeleon version as appropriate TIP When a new firmware version is released, the new version will be included in the next available Chromeleon version.
  • Page 185 • Maintenance and Service 3. Monitor the Audit Trail of the Instrument Configuration Manager program to see whether the firmware update was successful or failed. If the firmware update failed, turn the device off and on again ¨ and repeat the firmware update. If the firmware update fails repeatedly, contact Thermo Fisher ¨...

  • Page 186: Replacing The Doors

    • Maintenance and Service 7.14 Replacing the Doors When Damage of door TIP The maintenance procedures do not require that you remove the doors. If this should ever be required for a specific reason or procedure, follow the related steps in this section. Parts required Replacement door Follow these steps...
  • Page 187 • Maintenance and Service 2. Slightly tilt the door to the outside, away from the housing, and remove the door. 3. To install the door, align the door with the hinges on the housing. Be careful not to clamp tubing or capillaries between the door and the enclosure.

  • Page 188: Transporting Or Shipping The Device

    • Maintenance and Service 7.15 Transporting or Shipping the Device If you want to transport the device to a new location or if you need to ship the device, first prepare the device for transport and then move or ship the device as required. Follow the instructions in this section. Observe the following safety guidelines: CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely.

  • Page 189: Transporting The Device To A New Location

    • Maintenance and Service 5. Check the leak tray and drain port. If liquid is present, absorb the liquid with a tissue. 6. Remove the device or slide-in module from the system stack as required: Install the carrying handles and remove the device from the Vanquish system.

  • Page 190: Shipping The Device

    • Maintenance and Service 7.15.3 Shipping the Device Preparations Prepare the device for transport. See Preparing the Device for Transport (} page 188). Follow these steps 1. Follow the unpacking instructions in this manual in the reverse order. Use only the original packing material and shipping container. If the original shipping container is not available, appropriate containers and packing material can be ordered from the Thermo Fisher Scientific sales organization.

  • Page 191: Replacing The Slide-In Module

    • Maintenance and Service 7.16 Replacing the Slide-In Module 7.16.1 Removing the Slide-In Module CAUTION—Heavy Load, Bulky Device The device is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the device, observe the following guidelines: •...

  • Page 192: Returning The Slide-In Module

    • Maintenance and Service 3. Grasp the slide-in module by the leak tray and pull the module out of the enclosure by approximately 10 cm. Figure 63: Pulling out the slide-in module NOTICE The slide-in module can fall down when pulling it out of the enclosure too far.

  • Page 193: Installing The Slide-In Module

    • Maintenance and Service NOTICE Shipping the slide-in module improperly leads to damage to the device. Always ship the slide-in module as described in this operating manual. CAUTION—Possible Contamination Hazardous substances may have contaminated the device during operation and may cause personal injury to service personnel. •...
  • Page 194 • Maintenance and Service Follow these steps 1. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to insert the slide-in module properly into the enclosure in the next step.

  • Page 195: Setting Up The Slide-In Module

    • Maintenance and Service NOTICE • Verify that the screws are tightened. Pull the slide-in module by the leak tray towards the front and check whether the screws move. If they do not move, the slide-in module is installed properly. •...
  • Page 196 • Maintenance and Service Page 196 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 197: Troubleshooting

    • Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the device. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 197 Operating Manual...

  • Page 198: General Information About Troubleshooting

    • Troubleshooting General Information about Troubleshooting The following features help you to identify and eliminate the source for problems that may arise during operation of the device. NOTICE When the Vanquish system includes a Vanquish charged aerosol detector, the detector may affect the operation and/or performance of the pump.
  • Page 199 • Troubleshooting Chromeleon Audit Trail Messages If the device firmware detects a problem, the problem is reported to the Chromeleon software. The Chromeleon software logs information about all events related to instrument operation for the current day in an Audit Trail. The Audit Trail is named with the current date, using the format yyyymmdd.

  • Page 200: Messages

    • Troubleshooting Messages This section provides information about the messages that may appear in the Chromeleon Audit Trail during operation of the pump. For easier identification of the source for a problem, note the Device information that may appear in front of the message: Device Information Description [Main Device Name]...
  • Page 201 • Troubleshooting TIP If you are unable to resolve the problem following the instructions in this manual, or if you encounter a message not listed in the table, write down the code and wording of the message and contact us. For details, see the Contacting Us section at the beginning of this manual.
  • Page 202 • Troubleshooting Message and Code Description and Remedial Action Code 92 Data verification failed. • This may be a temporary error. Turn off the module. Wait for 5 seconds and turn on the module again. • The firmware may be defective. Update the firmware. Code 118 USB Buffer Overflow.
  • Page 203 • Troubleshooting Message and Code Description and Remedial Action Code 4100 Purge pressure limit exceeded A pressure of more than 5 MPa built up after a purge cycle has started. Perform the following sequence of steps: 1. Open the purge valve if it is not yet open. 2.
  • Page 204 • Troubleshooting Message and Code Description and Remedial Action Code 4116 The pressure signals are not The pressure calibration factor for the working piston is out of consistent. There may be air in the range. Perform these steps: working cylinder. 1. Verify that the degasser is working. 2.
  • Page 205 • Troubleshooting Message and Code Description and Remedial Action Code 4125 Degasser malfunction The vacuum level monitoring function detected insufficient degasser vacuum. Turn the pump off and on again. Check the degasser vacuum in the Chromeleon software. After about 1 minute, the setting should change from NotOk to Ok.
  • Page 206 • Troubleshooting Message and Code Description and Remedial Action Code 4156 Compression limit reached. The compression value was 100% during the last stroke. If the compression value is lower than 100% for several strokes afterward, the message "Compression back to normal" (code 4157) appears.
  • Page 207 • Troubleshooting Message and Code Description and Remedial Action Code 4241 An autosampler is required. To perform the procedure, an autosampler is required that is unambiguously defined. In the Chromeleon software, select the correct configuration for your system and connect all devices. Code 4242 The autosampler is offline.

  • Page 208: Testing The Pump For Leakage

    • Troubleshooting Testing the Pump for Leakage When If you suspect leakage in the pump When the leak sensor reports leakage, see Resolving Liquid Leaks (} page 209). Tests The following tests assist you in finding out whether leakage is present in the pump and they help you to locate the source of a leak: •...

  • Page 209: Resolving Liquid Leaks

    • Troubleshooting Resolving Liquid Leaks TIP If leakage is observed around the purge valve knob (when the purge valve is open) or on the purge outlet (when the purge valve is closed), the purge valve knob may be defective (see Replacing the Purge Valve Knob (} page 177)).

  • Page 210: Pressure Issues Or Shifts In Retention Time

    • Troubleshooting Pressure Issues or Shifts in Retention Time When you observe pressure issues or shifts in retention time, the following sections help you to identify and eliminate the source for the problem: • The pressure is not down to zero when the purge valve is open Pressure Not Zero with Open Purge Valve (} page 210).
  • Page 211 • Troubleshooting Follow these steps 1. In the Chromeleon software, check the Compression value for the pump heads (= CompressionDrv1). The values indicate the compression of the last stroke in percent. The compression value should be less than 100%. With isocratic conditions, the compression value should remain ¨...

  • Page 212: Testing The Static Mixer For Permeability

    • Troubleshooting 8.5.3 Testing the Static Mixer for Permeability TIP The information and references to the static mixer in this section apply equally to the inline filter if installed. When If you observe pressure pulsation, inconstant pressure, or high backpressure at the column and pump Additional items required •...

  • Page 213: Removing Persistent Air Bubbles

    • Troubleshooting Removing Persistent Air Bubbles If you are unable to remove air bubbles with purging, purge the pump against backpressure. Follow the procedure for running in new piston seals in Recommendations for New Piston Seals (} page 151). Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 213 Operating Manual...
  • Page 214 • Troubleshooting Page 214 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 215: Pump-Specific Information

    • Pump-Specific Information 9 Pump-Specific Information This chapter provides specific information about the pump, for example, the interior components and operating principle. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 215 Operating Manual...

  • Page 216: Introduction To This Chapter

    • Pump-Specific Information Introduction to this Chapter This chapter provides information that is specific to the pumps, for example the interior components and the operating principle: • Quaternary Pump and Dual Pump (} page 217) • Binary Pump (} page 228) • Isocratic Pump (} page 233) Page 216 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 217: Quaternary Pump And Dual Pump

    • Pump-Specific Information Quaternary Pump and Dual Pump This section provides specific information on the quaternary pump and the dual pump. TIP The dual pump combines two ternary pumps in a single enclosure. The two pumps are referred to as left pump and right pump. If not otherwise stated, the information in this section applies equally to the quaternary pump and the two pumps of the dual pump.

  • Page 218: Interior Components

    • Pump-Specific Information 9.2.1 Interior Components 9.2.1.1 Quaternary Pump The user-accessible components of the pump are located directly behind the front doors: Figure 65: Interior view Description Keypad with buttons for pump control Proportioning valve (4 channels) Tubing clips for routing solvent lines to the proportioning valve Pump head Purge/sensor unit including purge valve and system pressure sensor Leak sensor...
  • Page 219 • Pump-Specific Information 9.2.1.2 Dual Pump The user-accessible components of the pump are located directly behind the front doors: Figure 66: Interior view Description Proportioning valve (3 channels) (left pump) Tubing clips for routing solvent lines to the proportioning valve Keypad with buttons for pump control Pump head (left pump) Purge/sensor unit including purge valve and system pressure sensor (left pump)
  • Page 220 • Pump-Specific Information Description Static mixer (as part of mixing system, right pump) Pump block LED (right pump) Pump outlet (outlet of the static mixer) (right pump) Capillary mixer (as part of mixing system, left pump) Static mixer (as part of mixing system, left pump) Pump block LED (left pump) Pump outlet (outlet of the static mixer) (left pump) Page 220...

  • Page 221: Operating Principle

    • Pump-Specific Information 9.2.2 Operating Principle The picture illustrates how the pump operates: Figure 67: Operating principle Description Solvent options: • 4 solvents available with the quaternary pump (solvents A-D) • 3 solvents available with the dual pump (solvents A-C), with each of the two pumps Solvent degasser (vacuum degasser) (pump inlet) Proportioning valve...

  • Page 222: Dwell Volume Of The Pump

    • Pump-Specific Information 9.2.3 Dwell Volume of the Pump The gradient delay volume of an HPLC system is defined as the volume of the entire flow path between the point where the gradient is formed and the point where the mobile phase enters the column (see Gradient Delay Volume, Dwell Volume and Ripple (} page 236)).

  • Page 223: Setting The Solvent Composition

    • Pump-Specific Information 9.2.4 Setting the Solvent Composition This section describes how you set the solvent composition for the quaternary pump (solvents A to D). The steps and examples apply equally to the ternary pumps of the dual pump (solvents AR to CR and AL to CL).

  • Page 224: Synchronizing The Injection Time With The Pump Strokes

    • Pump-Specific Information 9.2.5 Synchronizing the Injection Time with the Pump Strokes To enhance the retention time precision with gradient applications, you can synchronize the injection time of the Vanquish autosampler with the strokes of the Vanquish pump. The synchronization ensures that all injections are performed at the same phase of the pump cycle.

  • Page 225: Dual Pump Specifics

    • Pump-Specific Information 9.2.6 Dual Pump Specifics Note the following: • The dual pump combines two ternary pumps in a single enclosure. • The two pumps are referred to as left pump and right pump. On the keypad, L refers to the left pump and R refers to the right ¨...
  • Page 226 • Pump-Specific Information 9.2.6.2 Monitoring the Solvent Consumption TIP When monitoring the liquid level in the solvent reservoirs (solvent consumption) as described in this section, the Chromeleon software calculates the liquid level. To monitor the liquid level in real time, based on real physical measurement, install the Vanquish Solvent Monitor.
  • Page 227 • Pump-Specific Information 9.2.6.3 Monitoring the Liquid Level in the Waste Container TIP When monitoring the liquid level in the waste container as described in this section, the Chromeleon software calculates the liquid level. To monitor the liquid level in real time, based on real physical measurement, install the Vanquish Solvent Monitor.

  • Page 228: Binary Pump

    • Pump-Specific Information Binary Pump This section provides specific information on the binary pump. See • Interior Components (overview) (} page 228) • Operating Principle (} page 230) • Dwell Volume of the Pump (} page 231) • Setting the Solvent Composition (} page 232) 9.3.1 Interior Components The user-accessible components of the pump are located directly behind the front doors: Figure 69: Interior view...
  • Page 229 • Pump-Specific Information Description Vacuum degasser (solvent A) Solvent selector (solvent B) Solvent selector (solvent A) Seal wash pump Tubing chase with tubing guides Pump block LEDs Capillary mixer (as part of mixing system) Static mixer (as part of mixing system) Pump outlet (outlet of the static mixer) Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 229...

  • Page 230: Operating Principle

    • Pump-Specific Information 9.3.2 Operating Principle The picture illustrates how the pump operates: Figure 70: Operating principle Description Solvent selector (pump inlet) Solvent degasser (vacuum degasser) Pump head, left, with Working cylinder (left pump head) Equilibration cylinder (left pump head) Pump head right, with Working cylinder (right pump head) Equilibration cylinder (right pump head) Purge/sensor unit, with...

  • Page 231: Dwell Volume Of The Pump

    • Pump-Specific Information 9.3.3 Dwell Volume of the Pump The gradient delay volume of an HPLC system is defined as the volume of the entire flow path between the point where the gradient is formed and the point where the mobile phase enters the column (see Gradient Delay Volume, Dwell Volume and Ripple (} page 236)).

  • Page 232: Setting The Solvent Composition

    • Pump-Specific Information 9.3.4 Setting the Solvent Composition For increased flexibility, the pump is shipped with two 3-port solvent selectors. They allow forming binary gradients from three solvents per channel. The solvent to be used for each channel is selected in the user interface.

  • Page 233: Isocratic Pump

    • Pump-Specific Information Isocratic Pump This section provides specific information on the isocratic pump. See • Interior Components (overview) (} page 233) • Operating Principle (} page 234) 9.4.1 Interior Components The user-accessible components of the pump are located directly behind the front doors: Figure 72: Interior view Description Keypad with buttons for pump control...

  • Page 234: Operating Principle

    • Pump-Specific Information 9.4.2 Operating Principle The picture illustrates how the pump operates: Figure 73: Operating principle Description Solvent Pump head, with Working cylinder Equilibration cylinder Purge/sensor unit, with Purge valve knob Purge outlet System pressure sensor Mixing system, serving as inline filter, with Capillary mixer Static mixer Pump outlet (outlet of the static mixer)

  • Page 235: Modifying The Pump For Specific Applications

    • Modifying the Pump for Specific Applications 10 Modifying the Pump for Specific Applications This chapter provides information about how you can modify the pump for specific applications. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 235 Operating Manual...

  • Page 236: Gradient Delay Volume, Dwell Volume And Ripple

    • Modifying the Pump for Specific Applications 10.1 Gradient Delay Volume, Dwell Volume and Ripple This section applies to binary pumps, quaternary pumps, and dual pumps. The gradient delay volume of an HPLC system is defined as the volume of the entire flow path between the point where the gradient is formed and the point where the mobile phase enters the column.

  • Page 237: Available Mixing Systems

    • Modifying the Pump for Specific Applications In the standard configuration, the pump has been optimized for the dwell volume and ripple. You can adapt the dwell volume or ripple to your requirements by replacing the mixing system with which the pump is shipped with a mixing system with a different volume: •...
  • Page 238 • Modifying the Pump for Specific Applications Description Remarks Mixing system, volume: 400 µL, comprising: Binary VC-pump, quaternary pump, dual pump: Mixing • Static mixer, volume: 350 µL system with which the pump is • Capillary mixer, volume: 50 µL shipped. The dual pump has two mixing systems.

  • Page 239: Exchanging The Mixing System

    • Modifying the Pump for Specific Applications 10.1.2 Exchanging the Mixing System When Changing to a mixing system with different volume Follow these steps 1. Remove the mixing system with which the pump is shipped (see the next section). 2. Install the new mixing system. Follow the sequence of steps for the mixing system that you want to install.
  • Page 240 • Modifying the Pump for Specific Applications 4. Pull the static mixer out of the mounting bracket. 5. Remove the assembly of static mixer and capillary mixer from the pump. 6. Disconnect the capillary mixer from the static mixer. 10.1.2.2 Installing a Mixing System with a Volume higher than 100 µL Parts required Static mixer as required for the mixing system (see...
  • Page 241 • Modifying the Pump for Specific Applications Figure 75: Removing the mounting bracket Description Mounting holes for mounting bracket With binary pump only: spacer sleeves Mounting bracket Mounting screws (Torx) 2. Using the screws shipped with the mounting bracket, attach the new bracket inside the pump (same position and orientation as the one you removed).
  • Page 242 • Modifying the Pump for Specific Applications 1. Connect the capillary mixer to the static mixer. Mind the direction of flow through the static mixer and capillary mixer (indicated by the arrow on the mixer). 2. Insert the assembly of static mixer and capillary mixer into the pump.
  • Page 243 • Modifying the Pump for Specific Applications 10.1.2.3 Installing the 100 µL Mixing System Parts required • Mixing system, volume: 100 µL, comprising a static mixer (volume: 75 µL) and a capillary mixer (volume: 25 µL) • Capillary to connect the pump to the autosampler Tools and additional items required •...
  • Page 244 • Modifying the Pump for Specific Applications Follow these steps 1. Connect the capillary mixer on the purge/sensor unit. Mind the direction of flow through the capillary mixer (indicated by the arrow on the mixer). TIP To facilitate installation of the static mixer, loosely tighten the capillary mixer on the purge/sensor unit so that you can easily access the free end of the capillary.
  • Page 245 • Modifying the Pump for Specific Applications 8. With Chromeleon 7.2.8 and later: In the Chromeleon software, set the CapillaryMixer property to the value indicated on the capillary mixer. 9. Apply the flow rate of your application and have the pump deliver for a short time.
  • Page 246 • Modifying the Pump for Specific Applications Installing the New Mounting Bracket 1. Unplug the system pressure sensor cable from the P-SYS connector. 2. Remove the seal wash detector assembly (funnel and detector). Grasp and pull the assembly upward by the funnel. The detector including the tubing and cable can remain connected to the funnel.
  • Page 247 • Modifying the Pump for Specific Applications Description Mounting holes for mounting bracket (covered by push rivets while no mounting bracket is installed in this position) Mounting bracket Mounting screws (Torx) Follow these steps 1. Connect the capillary mixer on the purge/sensor unit. Mind the direction of flow through the capillary mixer (indicated by the arrow on the mixer).
  • Page 248 • Modifying the Pump for Specific Applications 7. Connect the inline filter outlet to the autosampler injection valve (see Connecting the Pump and Autosampler (} page 80)). 8. In the Chromeleon software, set the StaticMixer property to the value indicated on the inline filter. If the property is not set to the correct value, the leak tests may not provide reliable results.

  • Page 249: Normal-Phase Compatible Solvents And Additives

    • Modifying the Pump for Specific Applications 10.2 Normal-Phase Compatible Solvents and Additives This section applies to VC‑pumps and VC‑autosamplers. In a Vanquish Core system, normal-phase (NP) compatible solvents and additives may be used if the pump and autosampler are modified with the components from the Normal Phase (NP) kit.
  • Page 250 • Modifying the Pump for Specific Applications Follow these steps 1. Replace the following seal wash lines (silicone tubing) with the NP compatible tubing: Seal wash lines that connect the seal wash reservoir to the ¨ peristaltic tubing in the pump: Follow the steps in the Operating Manual for the Vanquish autosampler.

  • Page 251: Considerations With Normal-Phase Compatible Solvents And Additives

    • Modifying the Pump for Specific Applications 10.2.2 Considerations with Normal-Phase Compatible Solvents and Additives When running applications with normal-phase compatible solvents and additives, observe also the information in this section on: • Seal wash liquid • Long-term shutdown • Routine and preventive maintenance •...
  • Page 252 • Modifying the Pump for Specific Applications Routine and Preventive Maintenance • Observe the information in Routine and Preventive Maintenance (} page 122). • Depending on the application, slightly increased abrasion may be observed with normal-phase piston seals. Therefore, consider testing the static mixer for permeability at shorter intervals (see Testing the Static Mixer for Permeability (} page 168)).
  • Page 253 • Specifications 11 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the device. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 253 Operating Manual...
  • Page 254 • Specifications 11.1 Performance Specifications For details on the performance specifications for the pumps, see the next sections. 11.1.1 Quaternary Pump and Dual Pump The pump performance is specified as follows: Type Specification* Operating principle Serial dual-piston pump Compressibility compensation Fully automated, independent of the composition of the mobile phase Flow range (settable) VC-pumps:...
  • Page 255 • Specifications Type Specification* Biocompatible VC-pumps: VF-pumps: Communication USB: 1 USB port (USB 2.0, "B" type connector) 1 USB hub with 3 ports (USB 2.0, "A" type connectors) I/O Interface: 2 Dig I/O ports (mini-DIN), each providing one input, one relay output, and one bidirectional input/output System Interlink: 2 system interlink ports (RJ45-8 connectors)
  • Page 256 • Specifications 11.1.2 Binary Pump The pump performance is specified as follows: Type Specification* Operating principle Serial dual-piston pump Compressibility compensation Fully automated, independent of the composition of the mobile phase Flow range (settable) VC-pump: VF-pump: 0.001 – 10 mL/min in 1 µL/min 0.001 –...
  • Page 257 • Specifications Type Specification* Communication USB: 1 USB port (USB 2.0, "B" type connector) 1 USB hub with 3 ports (USB 2.0, "A" type connectors) I/O Interface: 2 Dig I/O ports (mini-DIN), each providing one input, one relay output, and one bidirectional input/output System Interlink: 2 system interlink ports (RJ45-8 connectors) Control...
  • Page 258 • Specifications 11.1.3 Isocratic Pump The pump performance is specified as follows: Type Specification* Operating principle Serial dual-piston pump Compressibility compensation Fully automated, independent of the composition of the mobile phase Flow range (settable) 0.001 – 10 mL/min in 1 µL/min increments Flow accuracy ± 0.1% Flow precision...
  • Page 259 • Specifications Type Specification* Good Laboratory Practice (GLP) Predictive Performance functions for scheduling maintenance features procedures based on the actual operating and usage conditions of the pump. All system parameters logged in the Chromeleon Audit Trail. * Typical operating conditions for measurable specifications: Flow accuracy, flow precision, pulsation: 1 mL/min at 20 MPa or 2 mL/min at 40 MPa, water Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 259...

  • Page 260: Physical Specifications

    • Specifications 11.2 Physical Specifications The physical conditions of the device are specified as follows: Type Specification Range of use Indoor use only Ambient operating 5 °C - 35 °C temperature Ambient storage temperature -20 °C - 45 °C Ambient operating humidity 20% - 80% relative humidity (non-condensing) Ambient storage humidity Maximum 60% relative humidity (non-condensing) Operating altitude...

  • Page 261: Accessories, Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 12 Accessories, Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the device and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts.

  • Page 262: General Information

    • Accessories, Consumables and Replacement Parts 12.1 General Information The device must be operated only with the replacement parts and additional components, options, and peripherals specifically authorized and qualified by Thermo Fisher Scientific. Accessories, consumables, and replacement parts are always maintained at the latest technical standard.

  • Page 263: Ship Kit

    • Accessories, Consumables and Replacement Parts 12.2 Ship Kit The ship kit includes the items listed in the table. The kit content is subject to change and may vary from the information in this manual. See the content list included in the kit for the most recent information about the kit content at the time when the device is shipped.
  • Page 264 • Accessories, Consumables and Replacement Parts VF-P10 ship kit Item Quantity in shipment Silicone tubing, 3 m Syringe, plastic, 12 mL Tubing connector (90°-angled) Solvent line filter, filter holder (no filter frit included) Solvent line filter, filter frit, biocompatible, 10 µm Plugs and retaining guides for reservoir caps, kit including •...

  • Page 265: Dual Pump

    • Accessories, Consumables and Replacement Parts 12.2.2 Dual Pump For reordering information of the ship kit components, see Consumables and Replacement Parts (} page 273). VC-P32, VC-P33 ship kit Item Quantity in shipment Silicone tubing, 3 m Syringe, plastic, 12 mL Tubing connector (90°-angled) Solvent line filter, filter holder (no filter frit included) Solvent line filter, filter frit, 10 µm (pack of 10) Plugs and retaining guides for reservoir caps, kit including...

  • Page 266: Isocratic Pump

    • Accessories, Consumables and Replacement Parts Item Quantity in shipment Plugs and retaining guides for reservoir caps, kit including • Cap plug to close open holes in the reservoir cap (pack of 5) • Retaining guide to keep the liquid line in place in the reservoir cap (pack of 2) Fitting plug, Viper Tool kit, including...
  • Page 267 • Accessories, Consumables and Replacement Parts Item Quantity in shipment Tool kit, including • Open-end wrench, size 11 x 13 mm • Double-ended ring wrench, size 11 x 13 mm • Open-end wrench, size 1/4" x 5/16" • Hexagon wrench, size 6 •...
  • Page 268 • Accessories, Consumables and Replacement Parts Item Quantity in shipment Tubing bracket Solvent lines (pack of 4), from solvent reservoirs to pump degasser System interlink cable (RJ45), 0,5 m USB cable, type A to type B, high-speed, USB 2.0, cable length: 5 m VF-P20 ship kit Item Quantity in...

  • Page 269: Optional Accessories

    • Accessories, Consumables and Replacement Parts 12.3 Optional Accessories For accessories that are available as an option, see • Optional Accessories (Miscellaneous) (} page 269) • Optional Mixing Systems and Inline Filters (} page 270) 12.3.1 Optional Accessories (Miscellaneous) Solvent shut off valve Description Part No.

  • Page 270: Optional Mixing Systems And Inline Filters

    • Accessories, Consumables and Replacement Parts Normal-Phase (NP) kit Description Part No. Normal-Phase (NP) kit 6036.3972 To modify VC‑pumps and VC‑autosamplers for using normal-phase compatible solvents and additives in a Vanquish Core system. The kit includes the following items: NP compatible tubing and seals for use as both piston seals and seal wash seals.
  • Page 271 • Accessories, Consumables and Replacement Parts Description Part No. Static mixer, volume: 350 µL 6044.5310 (for total volume of mixing system: 400 µL) Binary VC-pump, quaternary pump: The pump is shipped with the mixing system with a volume of 400 µL. Dual pump: The pump is shipped with two mixing systems, each with a volume of 400 µL.
  • Page 272 • Accessories, Consumables and Replacement Parts Description Part No. Set inline filter, volume: 35 µL, including: 6044.3870 • Inline filter (static filter) volume: 10 µL • Capillary mixer, volume: 25 µL In addition, order the capillary to connect the pump to the autosampler. Capillary to connect the pump to the autosampler, for use with 6042.2330 the 100 µL mixing system and 35 µL inline filter set...

  • Page 273: Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 12.4 Consumables and Replacement Parts The following consumables and replacement parts are available for the pump. 12.4.1 Maintenance Kits The maintenance kits that are available for the pumps include all parts that are required for routine maintenance. •...
  • Page 274 • Accessories, Consumables and Replacement Parts VC-P20 Description Part No. Maintenance kit for VC-P20, including: 6040.1951A • Pump head seal (2 PTFE seals, different sized) • Silicone tubing (clear tubing) • Peristaltic tubing (white tubing) • Tubing connector (straight) (pack of 4) •...
  • Page 275 • Accessories, Consumables and Replacement Parts VC-P40 Description Part No. Maintenance kit for VC-P40, including: 6040.1950A • Pump head seal (2 PTFE seals, different sized) • Silicone tubing (clear tubing) • Peristaltic tubing (white tubing) • Tubing connector (straight) (pack of 3) •...
  • Page 276 • Accessories, Consumables and Replacement Parts VF-P20 Description Part No. Maintenance kit for VF-P20, including: 6040.1954A • Pump head seal (2 PTFE seals, different sized) • Silicone tubing (clear tubing) • Peristaltic tubing (white tubing) • Tubing connector (straight) (pack of 4) •...

  • Page 277: Pump Heads And Components

    • Accessories, Consumables and Replacement Parts 12.4.2 Pump Heads and Components • Pump Heads and Components (VC-Pumps) (} page 277). • Pump Heads and Components (VF-Pumps) (} page 278). 12.4.2.1 Pump Heads and Components (VC-Pumps) VC-P10 Description Part No. Pump head (complete assembly), including spacing tool 6045.3205 Piston seal (RP) (pack of 2) 6040.0304...
  • Page 278 • Accessories, Consumables and Replacement Parts Description Part No. Capillary kit, pump head, including: 6040.3001 • U-tube (pack of 2) • Capillary from pump head to purge/sensor unit (pack of 2) Seal-handling tool 6040.7158 VC-P21 and VC-P33 Description Part No. Pump head (complete assembly), including spacing tool 6044.3202 Seals (pack of 2) for use as both seal wash seals and piston seals...
  • Page 279 • Accessories, Consumables and Replacement Parts Description Part No. Capillary kit, pump head, including: 6044.5016 • U-tube (pack of 2) • Capillary from right pump head to purge/sensor unit • Capillary from left pump head to purge/sensor unit Seal-handling tool 6040.7158 Installation tool for Viper capillaries with torque toothing 6040.2314...

  • Page 280: Solvents And Wash Systems

    • Accessories, Consumables and Replacement Parts 12.4.3 Solvents and Wash Systems Reservoirs for solvents and wash liquids Description Part No. Reservoir, 1 L, including cap 2270.0012 Reservoir, 0.25 L, including cap 2270.0026 Cap for reservoirs, screw-cap (pack of 4) 6270.0013 Plugs and retaining guides for reservoir caps, kit including 6030.9101 •...
  • Page 281 • Accessories, Consumables and Replacement Parts Solvent line filters, solvent lines and tubing Description Part No. Solvent line filter, filter holder (pack of 6) (no filter frits included) 6268.0115 Solvent line filter, filter frits, 10 µm (pack of 10) for VF‑pumps (biocompatible frits) 6268.0111 VC‑pumps (stainless steel frits) 6268.0110...

  • Page 282: Mixing Systems

    • Accessories, Consumables and Replacement Parts 12.4.4 Mixing Systems Each mixing system comprises a static mixer and a capillary mixer. The volumes of both mixers determine the entire volume of the mixing system. The tables list the reordering part numbers for the components with which the pump is shipped from the factory.

  • Page 283: Interface Cables And Power Cords

    • Accessories, Consumables and Replacement Parts Description Part No. Syringe and tubing kit, including 6000.0010 • 5 plastic syringes • 3m silicone tubing Tool kit, including 6007.9304 • Open-end wrench, size 11 x 13 mm • Double-ended ring wrench, size 11 x 13 mm •...
  • Page 284 • Accessories, Consumables and Replacement Parts Page 284 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 285: Appendix

    • Appendix 13 Appendix This chapter provides additional information about compliance and the use of the digital I/O ports. Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Page 285 Operating Manual...

  • Page 286: Compliance Information

    • Appendix 13.1 Compliance Information 13.1.1 Declarations of Conformity CE Declaration of Conformity The device has satisfied the requirements for the CE mark and is compliant with the applicable requirements. cTUVus Compliance The cTUVus label on the device indicates that the device has satisfied the requirements for the cTUVus mark.

  • Page 287: Weee Compliance

    • Appendix 13.1.2 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive. It is marked with the following symbol: Figure 82: WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.

  • Page 288: Digital I/O

    • Appendix 13.2 Digital I/O The digital I/O ports (Dig I/O) can be used to exchange digital signals with external devices. Each port provides: • one digital input • one relay output • one bidirectional input/output Pin Assignment Figure 83: Digital I/O port Description Bidirectional input/output Relay output — Relay_NC (Normally Closed contact)
  • Page 289 • Appendix Wire Color Signal Name Signal Level Remarks Green Ground — Ground Reference potential Yellow Digital input — Input (low active): Digital input; reference potential is Input On: 0-0.4 V ground. Note the following: Off: 2.2-5 V • The maximum input voltage at the Pull-up resistor: 47 kΩ...
  • Page 290 • Appendix Page 290 Pumps C, Pumps F ( VC-Pxx, VF-Pxx) Operating Manual...

  • Page 291: Index

    Index Index pump ............ 125 cleaning ............ 126 accessories .......... 44, 261 compression ........... 101 optional ............. 269 compression values........ 210 ship kit............ 263 condensation.......... 50, 55 additives ............ 26 connectors............ 53 information .......... 26 consumables .......... 261, 273 use............... 92 cTUVus mark .......... 286 air bubbles (remove) ...... 104, 108 curve............... 101 algae .............. 92 audit trail............ 199 Audit Trail messages ........ 200 decontamination .......... 126 degasser mode .......... 101...
  • Page 292 Index flow .............. 97 leak sensor mode ........... 101 Flow (button)............ 88 leak test flow acceleration.......... 97 piston seals........ 159, 209 flow connections .......... 56 pump .......... 179, 209 flow deceleration .......... 97 pump (NP) ......... 250, 252 Flow LEDs ............ 88 purge valve knob........ 209 flow ramp ............ 97 seal wash system....... 130, 209 flush leakage ............ 209 degassing channels ........ 124...
  • Page 293 Index parts ............ 137 pump head seals ........ 157 operating parameters ........ 97 replace............ 138 operating principle ........... 33 pump head bushing........ 137 operation............ 39, 83 pump head seals control elements ......... 86 replace............ 157 interrupt............ 113 pump inlet .......... 221, 230 power on/off .......... 91 pump outlet ...  218, 220, 221, 229, 230, 233, 234 safety guidelines ......... 85 pump pressure (record) ........ 98 optimization ........... 110...
  • Page 294 Index maintenance .......... 119 Smart Standby .......... 113 operation............. 85 Smart Startup ........... 96 power cord .......... 50 solvent protective equipment ......... 22 chloride concentration........ 27 qualification of personnel ...... 22 information .......... 26 servicing ............ 119 pH range............ 27 safety symbols.......... 18, 19 use............... 92 seal wash body .......... 137 solvent composition ......... 99 seal wash cycle ........
  • Page 295 Index pressure issue ........... 210 retention time shift ........ 210 tubing ............... 59 tubing bracket .......... 57 tubing chase ............. 57 tubing guides............ 57 type label............ 19 Universal Serial Bus ........ 53, 54 unpack.............. 42 USB .............  53, 54 Viper knurl ............ 59 torque toothing........... 59 waste .............. 64 waste container (share) ......... 227 waste level ............. 227 waste level monitoring........ 100 WEEE .............. 287...
  • Page 296 www.thermofisher.com Thermo Fisher Scientific Inc. 168 Third Avenue Waltham Massachusetts 02451...

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