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CarboPac PA200
Thermo Scientific CarboPac PA200 Manuals
Manuals and User Guides for Thermo Scientific CarboPac PA200. We have
1
Thermo Scientific CarboPac PA200 manual available for free PDF download: Product Manual
Thermo Scientific CarboPac PA200 Product Manual (37 pages)
Columns For Dual EGC ICS-6000
Brand:
Thermo Scientific
| Category:
Laboratory Equipment
| Size: 1 MB
Table of Contents
Table of Contents
5
1 Introduction
7
Carbopac PA200
7
2 Installation
8
The Dionex High Pressure Ion Chromatography Systems
8
System Configuration
9
System Requirements
10
Installation of Disposable Electrode into a Dionex ICS-5000 ED Cell, Ph-Ag/Agcl Reference Electrode or Pdh Reference Electrode
10
System Void Volume
10
The Injection Volume
11
The 1 MM System Injection Volume, 0.4 Μl
11
The 0.4 MM System Injection Volume, 0.1 Μl
11
The Dionex Carbopac PA200 Guard Column
11
System Start-Up
11
ICS-6000 Dual EG Start-Up Procedure for Carbopac PA200, 1 MM Column
12
ICS-6000 Dual EG Start-Up Procedure for Carbopac PA200, 0.4 MM Column
13
Verification of Column Cleanliness
15
3 Operation
16
Dionex Carbopac PA200 Column Operational Parameters
16
The Best Operational Guidelines
16
Initial Check List
16
Purity Requirements and Preparation of Eluents and Standards
17
Deionized Water
17
Carbohydrate Sialyated N-Linked Alditol Standard
17
Sample Preparation
17
Method Development
18
System Re-Start
18
System Re-Start Procedure for Dionex Carbopac PA200, 1 MM Column
18
System Re-Start Procedure for Dionex Carbopac PA200 0.4-MM Column
19
4 Applications
20
Fetuin Oligosaccharide Alditol Profiling
20
Profiling of Inulin
22
Profiling of Galacto-Oligosaccharides
24
5 Troubleshooting Guide
26
High Back Pressure
26
Finding the Source of High System Pressure
26
Replacing Column Bed Support Assemblies for 1 MM Column
27
Filter Samples
27
High Background
28
Preparation of Eluents
28
A Contaminated Guard or Analytical Column
29
Poor Resolution
29
Loss of Column Efficiency
29
Shortened Retention Times
30
Loss of Resolution for Early Eluting Peaks
31
Spurious Peaks
31
No Peaks, Poor Peak Area Reproducibility or too Small Peak Areas
31
Large Baseline Dip in the Chromatogram
32
Unidentified Peaks Appear with Expected Analyte Peaks
32
Decreased Detection Sensitivity
32
Excessive Gradient Rise
33
Reconditioning or Replacement of the Gold (Disposable) Electrodes or Replacement of the Reference Electrode
33
Appendix A - Dionex Carbopac PA200 Column Care
34
Recommended Operation Pressures
34
Column Start-Up
34
Column Storage
34
Dionex Carbopac PA200 Column Cleanup
34
Appendix B - Quality Assurance Report (QAR)
36
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