Replacing A Column; Ion Source - Thermo Scientific TSQ 9000 Hardware Manual

1
Performing Routine Maintenance

Replacing a Column

Replacing a Column
Replacing a column in a TSQ 9000 system with no VPI or an AEI ion source
12 TSQ 9000 Hardware Manual
The procedure for replacing a column depends on your system configuration. If your system
does not have a VPI or has an AEI ion source installed, see
9000 system with no VPI or an AEI ion source"
vacuum probe interlock (VPI) and the ExtractaBrite ion source is installed, see
column on a TSQ 9000 system with a VPI and an ExtractaBrite ion source"
Note Some of the cleaning procedures in this section require the use of methanol. If
methanol is unavailable or prohibited, substitute LCMS-grade or GC-grade ethanol or
isopropyl alcohol. Do not use denatured ethanol as it may contain impurities that
contaminate the GC-MS system.
If your TSQ 9000 system has no VPI or has an AEI ion source installed on the system, follow
the instructions below to replace the column.

To replace the factory-installed column in the TSQ 9000 system in a system with no
VPI or with an AEI ion source installed
Note If you are running samples, stop the acquisition before powering off the system.
1. Cool down the GC oven and injector. See the GC documentation for information.
2. Open the TSQ Series Dashboard and click Shut Down.
During the shutdown procedure the vacuum and heaters lights will remain off. Once the
procedure is complete and the instrument is ready to be powered off, the power light will
turn amber and start blinking rapidly. At this point it is safe to power off the TSQ 9000
system.
3. On the left side of the instrument, push down on the power switch to power-off the
TSQ 9000 system.
4. Open the front door of the instrument.
5. Look behind the right side of the vacuum interlock shield and twist the vent valve knob
one and a half times in a counterclockwise direction to open the vent.
6. Remove the current column from the MS transfer line. To replace the column in the GC
inlet, follow the instructions in
7. Connect the column to the MS transfer line. When connecting the column to the
transfer line, you may use either the spring loaded transfer line nut with the graphite
Vespel™ ferrule or the regular transfer line nut
.
Note For best results, we recommend you use the spring loaded transfer line nut.
"Replacing a column in a TSQ
on page 12. If your TSQ 9000 MS has a
"Installing the Column in the GC Inlet"
"Replacing a
on page 22.
on page 28.
Thermo Scientific