v. (Optional) Tap the Display Map drop down and choose a color. When unmixing a Multi-
wavelength or Spectro image, the default display map for the component will be set to
this option.
vi. (Optional) Tap Select Group to display a tree that lists all available groups. Select an
existing group for your PA Region or create a new group.
vii. Tap Save when you are done.
If you chose to Multiplex your image, you h ave the option to check or uncheck each
o
PA Region and the ability to select what color the line of data will be displayed as on the
graph.
To Measure Blood Oxygenation in a PA-Mode (Oxy-Hemo) Loop
When you are analyzing an Oxy-Hemo sub-mode image, you can select specific regions of interest and
precisely measure the percentage level of blood oxygenation in that region.
The Vevo Imaging System provides two display types for Oxy-Hemo acquisition. Choose the appropriate
display before taking measurements:
OxyZated Display Type: Used to calculate and quantify oxygen saturation; also particularly useful for
l
studying the hypoxic state of tumor microenvironment to predict disease burden, studying
fetal/maternal physiology, and studying stroke/ischemia.
HemoMeaZure Display Type: Used to measure and quantify hemoglobin content; also particularly
l
useful for studying anemia.
To Complete an Oxygenation Measurement
1. Acquire an Oxy-Hemo image or load an Oxy-Hemo image to review.
2. Tap Measurements, then PA Region. An active anchor point for the measurement will be placed on
the image.
3. Drag the anchor point to the desired start point, and then tap Set.
4. Tap and drag the anchor point to trace the desired area.
5. When you are done drawing your measurement, lift your finger from the control panel and tap Done.
The completed Oxy-Hemo measurement appears.
Contrast Region
The Contrast Region measurement traces a region of interest in Nonlinear Contrast Mode. The Vevo
software then measures the total area of the region.
This measurement is available in Nonlinear Contrast Mode only.
Placing Contrast Region Measurements
You can draw up to 20 Contrast Region measurements on one image.
Session Tasks
Vevo F2 Imaging System User Manual
85
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