Part 2 Reading Imaging Plates ................ 5 1. Setting the Reading Conditions ..................6 2. Setting the IP on the IP Stage ....................8 3. Setting the IP Stage on the FLA-7000 ................8 4. Starting Reading ........................9 Part 3 Reading Fluorescent Samples ............13 1.
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2006. Mar 3. Installation (For Macintosh ) ...................50 4. Uninstallation (For Macintosh ) ..................50 Part 7 Troubleshooting ................... 51 1. Errors ..........................52 2. Warnings ..........................52...
Turn on the FLA-7000 and peripheral devices. Caution If an Imaging Plate is inserted in the FLA-7000 before turning it on, its scanned data cannot be guaranteed. The sensitivity of the Imaging Plate may deteriorate, based on the self-diagnosis of the FLA-7000.
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1. Starting the Image Reader 2006. Mar Make sure that the FLA-7000 has completed the warm-up. (Only the power lamp on the upper left panel on the front of the FLA- 7000 is lit up when the warm-up is completed.) Then, perform the procedures below.
Digitizing Mode Status Click when performing The status of the digitizing. FLA-7000 is displayed. Ready Method The FLA-7000 is Click this button to ready for scanning. register or erase combinations of lasers and filters. Preference... Filter Filter Module Laser...
1. Setting the Reading Conditions 2006. Mar 1. Setting the Reading Click the button. Conditions The Reader Settings window for the IP mode is displayed. : Return to the main window from the IP mode. Make these settings before reading IPs. Refer to the following explanations of reading conditions when making settings.
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1. Setting the Reading Conditions 2006. Mar Before starting reading, make sure that the LD 650 nm laser is shown and the IP filter is selected in the Setting field as shown below. In IP reading, the LD 650 nm laser and IP filter are selected automatically.
2. Setting the IP on the IP Stage/3. Setting the IP Stage on the FLA-7000 2006. Mar Estimated time until reading finishes. Size of the file. Drag and select the scanning area on the IP stage. Note: Select this to specify the reading area based on the 2.5 cm grid lines on the IP stage.
4. Starting Reading 2006. Mar 4. Starting Reading Click the button to start reading. The scanned area is displayed in the real-time window, as shown below. The stage is read from the left towards the right. Reading may be finished at any time before the whole scanning area has completed reading.
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4. Starting Reading 2006. Mar When you want to change the display parameters of the real-time window, refer to the explanations below. : Select the type of tone curve from the pull- down menu. Exponential: The exponential tone curve is used to adjust gradations.
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Click the button to return to the first Reader Settings window. Do not open the stage door of the FLA-7000 until the stage has completely returned. If it is opened, close it immediately. When scanning finishes, the buttons...
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4. Starting Reading 2006. Mar Note: If the photo-multipliers (PMT) are replaced with a multi-alkali PMT, the IP mode switches from that of the regular IP mode to a PMT voltage adjustment mode. The PMT voltage adjustment mode differs from the regular IP mode in that a sensitivity level that matches the scanned sample can be set independently.
1. Setting the Reading Conditions 2006. Mar 1. Setting the Reading Click the button. Conditions The Reader Settings window for the Fluorescence mode is displayed. : Return to the main window from the Fluorescence mode. Make these settings before reading fluorescent samples. Refer to the following explanations of reading conditions when making settings.
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1. Setting the Reading Conditions 2006. Mar Note: When the number of scans is between 2 and 4, the laser name and scan number are automatically added to the specified name. 000- 635- 1 File Laser Scan name name number Example: If reading is set with the LD 635 nm laser, LD 473 nm laser, LD 635 nm laser, and LD 635 nm laser, with "test"...
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1. Setting the Reading Conditions 2006. Mar : Set the pixel size for reading. Click to select from one of the 4 types, as shown on the left. A sample with a smaller pixel size is analyzed more finely. 25 µm Short Reading Time Long...
2. Setting a Fluorescent Sample on the FLUOR Stage/3. Setting the FLUOR Stage on the FLA-7000/4. Starting Reading 2006. Mar : Use this button when saving the reading conditions in a file. You may save reading conditions that are used frequently with this...
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4. Starting Reading 2006. Mar The scanned area is displayed in the real-time window, as shown below. The stage is read from the left towards the right. Reading may be finished at any time before the whole scanning area has completed reading.
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Click the button to return to the first Reader Settings window. Do not open the stage door of the FLA-7000 until the stage has completely returned. If it is opened, close it immediately. When scanning finishes, the buttons become active, but the button is grayed out until the stage has completely returned.
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4. Starting Reading 2006. Mar Finish reading. Before turning off the power of the FLA-7000, shut down the Image Reader software.
1. Setting the Reading Conditions 2006. Mar 1. Setting the Reading Click the button. Conditions The Reader Settings window for the Digitizing mode is displayed. : Return to the main window from the Digitizing mode. Make these settings before reading digitized samples. Refer to the following explanations of reading conditions when making settings.
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1. Setting the Reading Conditions 2006. Mar : Select the Method that corresponds with the sample from the pull-down menu. The Method to be used for reading is displayed. The contents of the laser and filter combination to be used for reading are displayed. Note: To use the Digitizing mode, the LD 473 nm laser must be loaded and the Y520 filter must be registered, or the SHG 532 nm laser...
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1. Setting the Reading Conditions 2006. Mar Estimated time until reading finishes. Size of the file. Drag and select the scanning area on the FLUOR stage. Note: Select this to specify the reading area based on the 2.5 grid lines on the FLUOR stage. Select this to specify the reading area arbitrarily.
2. Setting a Digitized Sample on the FLUOR Stage/3. Setting the FLUOR Stage on the FLA-7000/4. Starting Reading 2006. Mar 2. Setting a Digitized Sample After setting the digitized sample on the FLUOR stage, place the on the FLUOR Stage fluorescent plate for digitizing on top of it.
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4. Starting Reading 2006. Mar Note1: If you click Stop during reading, the part that has not been read yet will be saved as an image with a data value of 0 (light intensity of 0). Note2: When you click the button, reading itself is canceled.
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Click the button to return to the first Reader Settings window. Do not open the stage door of the FLA-7000 until the stage has completely returned. If it is opened, close it immediately. When scanning finishes, the buttons...
1. Registering Laser and Filter Combinations (Method) 2006. Mar 1. Registering Laser and Note: Filter Combinations Method settings are registered by a serviceman upon installation. (Method) Under normal circumstances, it is not necessary to register these settings. You may register, change, and delete laser and filter combinations. Click the button on the main window.
Under normal circumstances, it is not necessary to register these Image Reader settings. After exchanging the filters of the FLA-7000, you must register the exchanged filters in the Image Reader. Note: If filters are not registered in the Image Reader, they are not displayed in the Image Reader window, even if they are physically set in the FLA-7000.
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: Use this button to delete a registered filter. Default filters ([**]) cannot be deleted. 2a-2 Exchange the filter of the FLA-7000. For instructions on exchanging the filter of the FLA-7000, see the Fluorescent Image Analyzing System FLA-7000 Operation Manual.
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2. Filter Module Settings (Filter Module) 2006. Mar 2a-3 Click filter position No. 2. A red frame appears around the selected filter position. 2a-4 Select [605DF40] from the Filter List. The selected item is highlighted in blue. 2a-5 Click the button.
2. Filter Module Settings (Filter Module) 2006. Mar Filter position No. 2 changes to [605DF40]. Note: Click the button to keep the filter position empty after removing a filter. 2a-6 Click the button. You may save four types of filter combinations that are currently displayed. Saving the Four Types of Filter Combinations Note:...
2. Filter Module Settings (Filter Module) 2006. Mar Recalling Four Types of You may recall four types of filter combinations that are currently registered. Filter Combinations 2c-1 Click the button on the main window. 2c-2 Click the button. The following dialog box is displayed. 2c-3 Select the name of the filter combination you want to recall, and click the...
3. Other Settings (Preference...) 2006. Mar 2d-3 Select the filter name and icon color you want to register, and click button. The filter is newly registered. Note: To delete or edit the register filter name, select the filter name and click the button.
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Note: Registration of the correction settings list is performed by a serviceman. Please contact the dealer where you purchased the FLA-7000, or contact Fuji Photo Film. Does not use ND filter for adjusting light intensity. Uses ND filter for adjusting light intensity.
3. Other Settings (Preference...) 2006. Mar If select Quick, reading time would become shorter. However, noise would stand out during reading. The reading time varies depending on the setting image size and the scanning area. In case of reading the whole area of FLUOR stage, the reading time are as follows: <...
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3. Other Settings (Preference...) 2006. Mar 3b-3 Refer to the explanations below and click a radio button. The standard file format of the Fuji Film BAS/FLA series. Each file is saved as a combination of an information file (inf file) and a raster file (img file).
Installation of FUJI USB Function driver Installation of Image Reader FLA-7000 software Note: 1a. Installation of FUJI USB The computer and FLA-7000 should not be connected with a USB cable Control Driver during the operation. 1a-1 Open the control panel and click “Printers and Other Hardware”.
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® 1. Installation (For Windows 2006. Mar 1a-4 Select “Yes, I have already connected the hardware” and click the “Next” button. 1a-5 Select “Add a new hardware device” and click the “Next” button. 1a-6 Select “Install the hardware that manually select from a list [Advanced]”...
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® 1. Installation (For Windows 2006. Mar 1a-7 Select “Show All Devices” and click the “Next” button. 1a-8 Click “Have Disk...” button. 1a-9 Click “Browse...” button. 1a-10 Set the folder in which a file is saved to FLA-7000 CD-ROM.
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® 1. Installation (For Windows 2006. Mar 1a-11 Open the FUJIUSB control folder. 1a-12 Select a DevMng.inf file and click the “Open” button. 1a-13 Click the “OK” button. 1a-14 Click the “Next” button.
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® 1. Installation (For Windows 2006. Mar 1a-15 Click the “Next” button. 1a-16 Click the “Continue Anyway” button. 1a-17 Click the “Finish” button.
® 1. Installation (For Windows 2006. Mar 1b. Installation of FUJI USB 1b-1 Connect the USB cable and turn ON the power switch of the FLA- Function Driver 7000. Note: Perform this operation, or the personal computer may be reset. 1b-2 Click the “Next”...
® 1. Installation (For Windows 2006. Mar Installation of 1c-1 Insert the installation CD-ROM of FLA-7000. Image Reader FLA-7000 1c-2 Click the setup icon. Software 1c-3 Click the “Next” button. 1c-4 Click the “Install” button. 1c-5 Click the “Finish” button.
2006. Mar 2. Uninstallation Open the control panel and select “Add or Remove program”. ® (For Windows Select Image Reader FLA-7000 and click the “Remove” button to be deleted. ➟ A progress bar is displayed and uninstallation is started. Note: Connection files created after installation of the reader software are deleted.
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Click the “Upgrade” button on the “Installation Type” screen. Provide the name and password of the administrator. Click the “close” button on the “Finish Up” screen. 4. Uninstallation Move the FLA-7000 folder contained in the Application folder on the Machintosh HD to your Trash bin. (For Macintosh Note: Connection files created after installation of the reader software are deleted.
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1. Errors/2. Warnings 2006. Mar 1. Errors An error is the condition in which all of the reading modes of the FLA-7000 cannot be used. When a warning occurs, the indicator lamps make a sound (“Be-beep, be- beep, be-beep”). An error message dialog box is displayed on the Image Reader screen displayed on the computer.
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2. Warnings 2006. Mar Laser error occurred. Meaning: Use other lasers. • A laser error has occurred. • Use a different laser. Countermeasure: • An error has occurred with the laser. • If an error occurs even after restarting the instrument, call a serviceman.
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Note: If an error message is displayed, a serviceman should take the countermeasures to resolve the trouble. Please contact the dealer where you purchased the FLA-7000, or contact Fuji Photo Film. Fuji Photo Film Co., Ltd. 2-26-30 Nishi Azabu, Minato-ku...