Install Run Troubleshooting - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Appendix A Troubleshooting
A

Install run troubleshooting

Install run troubleshooting

See also "QC errors—Sequencing or fragment analysis install run"
Observation
Install run fails (sequencing or
fragment)
Install run—no signal is
observed
Fragment install run fails
because all expected peaks
are not detected
498
Possible cause
A capillary is blocked.
Insufficient filling of capillary
array.
The install run standards
and reagents are expired or
degraded.
Bubbles are present in the
pump channels.
Crystal deposits are present
in the polymer because the
polymer was not at room
temperature during the run.
Incorrect preparation of
reagents and standards.
Bubbles are present in the
sample wells.
Capillary tips are not touching
the sample or are hitting the
bottom of the wells.
Polymer has been installed on
the instrument for >3 days.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
on page 494.
Recommended action
Run the Fill array with polymer maintenance
wizard. (In the home screen, tap
Actions4Maintenance4Maintenance
wizards4Fill array with polymer.)
Check for broken capillaries.
Run the Fill array with polymer maintenance
wizard. (In the home screen, tap
Actions4Maintenance4Maintenance
wizards4Fill array with polymer.)
Use fresh standards and reagents. Use fresh
™
Hi‑Di Formamide. See "Hi‑Di
storage and usage" on page
Run the Remove bubbles maintenance
wizard. (In the home screen, tap
Actions4Maintenance4Maintenance
wizards4Remove bubbles.)
Allow the polymer to reach room temperature.
Do not heat it.
Prepare fresh reagents and standards. Use
™
fresh Hi‑Di Formamide.
Centrifuge the plate before running.
Contact Technical Support.
Install fresh polymer, then repeat the install
run.
™
Formamide
28.