Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 296

Chapter 11 Manage library resources on the instrument
11
Analysis settings library
Description
Size calling method
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• Local Southern (default)—Determines the fragment sizes using the reciprocal
relationship between fragment length and electrophoretic mobility.
• Global Southern—Compensates for standard fragments with anomalous
electrophoretic mobility (similar to least squares methods).
• Second Order Least Squares—Uses regression analysis to build a best-fit size
calling curve.
• Third Order Least Squares—Uses regression analysis to build a best-fit size
calling curve.
• Cubic Spline—Forces the sizing curve through all the known points of the
selected size standard.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Setting