Inspect The Data-Troubleshooting Procedures; View The Raw Data And The Ept Plot; Check Size Standard Quality (Fragment Analysis) - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Appendix A Troubleshooting
A
Inspect the data—Troubleshooting procedures
Tap REMOTE SESSION to return to the remote support session.
Tap View to return to the chat.
6. To end the remote support session, tap the active session, then tap Disconnect.
A Technical Support representative can also end the remote support session. REMOTE SESSION
is not displayed after a session has been disconnected.
Inspect the data—Troubleshooting procedures

View the raw data and the EPT plot

View the raw data and EPT plot to troubleshoot issues with a run.
The EPT view (ElectroPhoresis Telemetry) shows instrument data conditions (current, temperatures,
electrophoresis voltage) as a function of time.
This EPT plot is useful for troubleshooting problems with specific runs.
1. In the home screen, tap
2. Tap the plate of interest, then tap View.
3. Tap a well, tap View, then tap the Raw tab.
4. Tap the EPT tab.

Check size standard quality (fragment analysis)

This procedure describes how to check the size standard quality in the instrument software.
1. In the home screen, tap the plate position for an active run, or tap
2. Tap a fragment analysis plate name, then tap View.
QC and SQ values are listed for each well. For information on how these values are determined,
see "Sample QC and quality alerts"
A QC indicates all QC criteria were met. An SQ of >0.75 is a passing result.
If any wells have a
review the data and the details for the well as described below.
476
Actions4Run history.
on page 172.
or QC (indicating that a quality alert was triggered), or an SQ of ≤0.74,
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Actions4Run history.