Results Groups Library; Results Group Overview - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

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Description
Peak window size
Polynomial degree

Results Groups library

Results group overview

A results group defines the folder name where sample data files are stored.
If you do not specify a results group, the default results group is used: StartRuntime.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Enter a window width in data points for peak detection sensitivity. If more than one
peak apex is within the window, all are labeled as a single peak. Note the following:
• The maximum value is the number of data points between peaks.
• The setting is limited to odd numbers.
To increase peak detection sensitivity: Increase polynomial degree, decrease peak
window size. To decrease peak detection sensitivity: Decrease polynomial degree,
increase peak window size.
Polynomial degree cannot be greater than the peak window size.
Adjust to affect the sensitivity of peak detection. You can adjust this parameter to
detect a single base pair difference while minimizing the detection of shoulder effects
and/or noise.
The peak detector calculates the first derivative of a polynomial curve fitted to the
data within a window that is centered on each data point in the analysis range.
Using curves with larger polynomial degree values allows the curve to more closely
approximate the signal and, therefore, captures more of the peak structure in the
electropherogram.
Chapter 11 Manage library resources on the instrument
Setting
11

Results Groups library

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