Analysis Settings Library; Analysis Settings Overview - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Chapter 11 Manage library resources on the instrument
11

Analysis settings library

(continued)
Setting
Uniform binning
Off-scale recovery

Analysis settings library

Analysis settings overview

• Sequencing—Analysis settings define the settings used by the sequencing basecallers to assign
basecalls to each detected peak and assign a quality value.
• Fragment analysis—Analysis settings define the settings for peak detection and sizing.
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This option is available for matrix standard custom dye sets. Custom dye sets are any
dye sets that are not available from Thermo Fisher Scientific, or any user-created dye
set definitions.
Variable binning uses bin sizes that are optimized for the dyes. Uniform binning uses
bins of the same size for all dyes.
A custom dye set inherits the binning setting from the system dye set it was copied
from. System dye sets for fragment analysis D, F, G5, J6, and J6T use variable
binning.
If variable binning does not yield acceptable results, select Uniform binning for the
custom dye set.
The off‑scale recovery feature can be used to decrease data loss caused by an
occasional off-scale peak.
This feature can accurately estimate peaks up to 65,000 RFU when the frequency of
off-scale data is relatively low.
This feature is enabled by default in all system dye sets for fragment analysis.
If you do not want to use this feature, create a custom dye set, then deselect this
option.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Description