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(continued)
Matrix Standards Kit, BigDye
Sequencing Kit or the BigDye
Matrix Standards Kit, BigDye
[1]
Cycle Sequencing Kit
[1]
Alternatively, you can save the spectral calibration from a sequencing install run. See "Perform an install run"
Prepare the standard plate
IMPORTANT! Do not use warped or damaged plates.
1. Load the standards in any injection position in the plate.
The example below shows injection position 1, but you can specify the starting well for an injection
position. For information on other injection positions, see "Plate and strip-tube layout"
8-capillary
96-well plate
A1 through H1
1
2
A
1
B
2
C
3
4
D
E
5
F
6
G
7
H
8
2. Briefly centrifuge the plate that contains the standards.
3. Verify that each standard is positioned correctly in the
bottom of its well.
IMPORTANT! If the contents of any well contain
bubbles or are not located at the bottom of the well,
briefly centrifuge the plate, remove the plate from the
centrifuge, and verify that each standard is positioned
correctly in the bottom of its well.
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Name
™
Terminator v3.1 if you use the BigDye
™
Terminator v3.1 Cycle Sequencing Kit
™
Terminator v1.1 if you use the BigDye
24-capillary
96-well plate
A1 through H1, A2 through
H2, and A3 through H3
1
2
3
4
A
1
2
3
4
5
6
B
7
8
9
C
10
11
12
D
13
14
15
E
F
16
17
18
G
19
20
21
H
22
23
24
Chapter 12 Run calibrations and install checks
™
Direct Cycle
[1]
™
Terminator v1.1
24-capillary
384-well plate
Note: 384-well plates are not supported on 8‑capillary
instruments.
Columns 1, 3, and 5 in rows A, C, E, G, I, K, M, O
1
4
7
10
13
16
19
22
Run a spectral calibration
Cat. No.
4336974
4337449
on page
333.
on page
2
3
5
6
8
9
11
12
12
14
15
12
17
18
12
20
21
12
23
24
12
12
75.
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Table of Contents
Troubleshooting
