Run A Blanking Cycle - Thermo Scientific NanoDrop One User Manual

Run a Blanking Cycle

Thermo Scientific
Run a blanking cycle to verify the following:
• instrument is operating normally (with flat baseline)
• pedestals are clean (i.e., no dried-down sample material on pedestals)
• absorbance contribution of buffer solution you plan to use for sample analyses
Supplies needed
• lint-free laboratory wipes
• calibrated precision pipettor (0–2 µL)
• buffer solution for evaluation
To run a blanking cycle
For quick demonstration, watch multimedia training
for Suitability.
NOTICE
• Do not use a squirt or spray bottle on or near the instrument as liquids will
flow into the instrument and may cause permanent damage.
• Do not use hydrofluoric acid (HF) on the pedestals. Fluoride ions will
permanently damage the quartz fiber optic cables.
9
Learning Center
Prepare Samples and Blanks
Evaluating a Blanking Solution
NanoDrop One User Guide 207