Table of Contents
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Appendix A Troubleshooting
A
Troubleshooting workflow
■
Spectral calibration troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 523
■
Thermo Fisher
■
Voice command troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 525
Troubleshooting workflow
Follow this general workflow when you are troubleshooting.
Inspect the data
1.
Review the analyzed data
2.
Review the raw data, then review the EPT plot
3.
(Fragment analysis) Check size standard quality
4.
Confirm that the correct injection settings were used to generate the data
(run module, dye set, analysis settings, and size standard definition (fragment
analysis)). See the following sections:
•
"Sequencing results and well details"
•
"Fragment analysis results and well details"
Inspect the samples
1.
Confirm that the correct samples are present in the wells that are specified in
the plate file.
2.
Ensure that the samples are at the bottom of wells and that no bubbles are
present.
Inspect the instrument
1.
Check that capillary tips are not bent or damaged.
2.
Check the pump for bubbles or debris
3.
Check consumables status (see "Check consumables status"
4.
Follow any troubleshooting instructions displayed in the instrument error
messages or plate alerts.
5.
Perform any pending maintenance tasks (see "Review upcoming maintenance"
on page
64).
Request assistance
1.
Export the log files generated by the instrument
2.
Request assistance
3.
If instructed to do so, start a remote support session
472
™
Connect troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 524
Troubleshooting workflow
(page
473).
SeqStudio
™
(page
476).
(page
476).
on page 176
on page 180
(page
480).
on page
(page
482).
(page
475).
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
66).
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Table of Contents
Troubleshooting
