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The software reports one of the following statuses.
• Optimized successfully for this run on Date_Timestamp—The spectral calibration was
optimized during auto-spectral calibration using spectral data from the sample and the optimized
auto-spectral dye matrix was applied to the sample and saved for future use.
• Calibration from sample with timestamp Date_Timestamp—Although the software was not
able to optimize the calibration with information from the current sample, it used the most recently
saved optimized auto-spectral dye matrix for the capillary, and applied it to the sample. The most
recently saved optimized auto-spectral dye matrix is more current than the dye matrix from a
manual calibration.
• Calibration from manual calibration performed on Date_Timestamp—The software was not
able to optimize the calibration with spectral data from the current sample and did not find a saved
auto-spectral dye matrix for the capillary position. The dye matrix from the most recent manual
spectral calibration was applied to the sample.
Note: If the calibration status for all samples in a run is reported as "Calibration from manual ...",
and you observe pull up-peaks, perform a manual spectral calibration, then repeat the run.
IMPORTANT! Spectral calibration status is reported for informational and tracking purposes only. This
calibration status does not reflect the quality of the sample or affect the results. However, use of the
manual spectral calibration instead of the auto-spectral calibration can increase the likelihood of pull-up
peaks.
View real-time results in the home screen
For more information on reviewing results, see:
• "View real-time results in the Run Queue screen"
• "View results for a completed run (Run history)"
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Chapter 6 Start and monitor a run
View real-time results in the home screen
on page 162
on page 170
6
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Table of Contents
Troubleshooting
