Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 80

Chapter 3 Prepare the samples
3
Prepare and assemble sample plates
1. If the reagents of any tube contain bubbles or are not located at the bottom of the well,
briefly centrifuge the plate, remove the plate from the centrifuge, then verify that each sample
is positioned correctly in the bottom of its well.
2. Place the tubes in the 96-well tray.
Ensure that the orientation of the 8-strip tubes matches the plate layout of the plate file and the
well numbers of the 96-well tray. If the orientation is incorrect, the samples on the plate will not
match the sample information in the plate file. Data will be identified with incorrect well IDs and
sample information.
3. For 8-strip standard tubes, place the retainer on the tubes.
4. Align the holes in the septum strip with the retainer (8-strip standard tube) or the tubes (8-strip fast
tube), then firmly press down.
IMPORTANT! The array tips will be damaged if the plate retainer and septum strip holes are not
aligned.
5. Place the tray-tube-retainer assembly into the plate base.
6. Snap the plate retainer (cover) onto the plate base.
IMPORTANT! Ensure that the plate retainer is firmly snapped in place on top of the plate (see
"Prepare the plate assembly"
assembly can become jammed in the drawer.
7. Verify that the holes of the plate retainer and the septum strip are aligned. If the holes are not
aligned, take the assembly apart, then re-assemble.
384-well plate assembly
IMPORTANT! Use the correct 384-well plate retainer and plate base with the same catalog number.
Using the wrong plate base may affect performance. See Appendix D, "Catalog numbers" for plate
assembly specifications and catalog numbers.
1. If the reagents of any well contain bubbles or are not located at the bottom of the well, briefly
centrifuge the plate, remove the plate from the centrifuge, then verify that each sample is
positioned correctly in the bottom of its well.
2. Place the plate into the plate base.
3. Place the septum on the plate and press down to seat.
Note: Reuse of the septum is not recommended.
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on page 77). If the retainer is not snapped in place, the plate
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide