Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 339

Reference sequence of the sequencing install standard
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Basecalls from the run. Basecalls that do not match the known bases in the reference sequence are marked in
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red.
QV20 CRL is referred to as CRL on the Capillaries tab.
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Alignment range. The starting base is determined by the software. In the example above, the alignment range
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starts at 27 because of the contiguous bases before 27 that do not match the reference sequence.
Accuracy is referred to as %RL basecall on the Capillaries tab.
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SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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Chapter 12 Run calibrations and install checks
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Perform an install run
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