Inspect The Instrument -Troubleshooting Procedures; View The Instrument Sensor Details And Ept Plot; Check The Pump For Bubbles Or Debris - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Appendix A Troubleshooting
A
Inspect the instrument —Troubleshooting procedures
Although no alerts were triggered, the low SQ should be investigated. In this example, the low SQ is
caused by the issues with peak resolution later in the electropherogram.
Inspect the instrument —Troubleshooting procedures

View the instrument sensor details and EPT plot

The EPT view (ElectroPhoresis Telemetry) shows instrument data conditions (current, temperatures,
electrophoresis voltage) as a function of time.
This EPT plot is useful for troubleshooting instrument issues that are not related to runs.
1. In the top-right of the home screen, tap
2. Tap
.
3. Tap View sensor details.
4. Tap View EPT.
5. Tap Start to generate the EPT plot.

Check the pump for bubbles or debris

1. Examine the fluidics pathway for bubbles or debris.
Check for small opaque or white debris that could be caused by polymer precipitation or
crystallization (typically observed only under cold temperatures). Check for dark particles or
specks of dust or dried polymer that could originate from the array-port end of the capillary array.
2. Ensure that all bubbles are removed from the fluidic pathway in the following locations:
• Pump channels (Figure 23)
• Anode channels (Figure 24)
480
(Dashboard).
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide