Estimated Install Run Times; Prepare For The Install Run - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Chapter 12 Run calibrations and install checks
12
Perform an install run

Estimated install run times

Note: The run times listed below do not include the time for the oven to preheat.
Install standard
BDT v1.1
BDT v3.1
Fragment
[1]
A 50-cm configuration is not supported.
The number of capillaries on the instrument does not affect run time.

Prepare for the install run

Prepare the instrument
1. Display the consumables dashboard by tapping
screen (if the consumables dashboard is not displayed, tap
2. Check consumables status (see "Check consumables status"
• Consumables are not expired
• Adequate injections remain for consumables
IMPORTANT! For install runs, use polymer that has been on the instrument ≤3 days. Slight peak
shifts can occur with polymer that has been on the instrument >3 days.
3. Ensure that buffer levels are at the fill lines (see "Check buffer fill levels"
4. Check the oven and detection cell temperature to ensure they are at the correct temperature (see
"Preheat the instrument oven and the detection cell"
5. Check the pump assembly for bubbles and run the Remove Bubble wizard if needed (see
"Remove bubbles from the polymer pump (wizard)"
330
Polymer-capillary length configuration
™
POP-4
[1]
36 cm 36 cm
Not available 66 minutes
Not supported 66 minutes
60 minutes Not supported
SeqStudio ™
™
POP-6
50 cm
135 minutes
90 minutes
Not supported
(Dashboard) at the top right of the home
on page
on page 377).
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
™
POP-7
36 cm
~40 minutes 125 minutes
~40 minutes
~40 minutes
or ).
on page 66). Ensure that:
on page 70).
68).
50 cm
40 minutes
40 minutes