Qc Errors-Spectral Calibration (Manual) - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Appendix A Troubleshooting
A
QC error and alert troubleshooting
QC errors—Spectral calibration (manual)
QC error
Insufficient number of dye spectra
detected.
492
Description
An incorrect calibration standard or
setting was used for calibration.
The calibration standard has
degraded or was incorrectly
prepared.
Figure 94 shows an example where
a peak is missing, possibly due to
degraded matrix standard.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Action
• Ensure that the matrix standard
or sequencing standard that you
use matches the dye set you
select for calibration.
• Sequencing calibration—
– Ensure that you select the
dye set from the Matrix tab
if you are calibrating with a
matrix standard.
– Ensure that you select
the dye set from the
Sequencing tab if you
are calibrating with a
sequencing standard.
Ensure that all of the following
conditions are met:
• The calibration standard is not
expired.
• The calibration standard is
prepared and stored as
specified in the product
information sheet for the
calibration standard.
• The calibration plate was
prepared within 24 hours of the
calibration run.
• Ensure that you used fresh
™
Hi‑Di Formamide. See "Hi‑Di
Formamide storage and usage"
on page 28. Older formamide
can break down to generate
formic acid, which can degrade
the calibration standard.
• Ensure that plate septa are not
being reused.
™