Create A New Size Standard - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Chapter 11 Manage library resources on the instrument
11
Size standards library
(continued)
Size standard definition
GS600LIZ(60-600)+Normalization
GS600LIZ+Normalization
[1]
The 250-bp peak is not included in the size standard definition because it is sensitive to small temperature variations.

Create a new size standard

1. In the home screen, tap
2. Tap Actions4Create new.
Note: You can also create a new size standard by opening, then copying, a factory-provided or
user-created size standard.
3. Enter a Size Standard Name.
4. Select a dye color, then tap Next.
5. Specify the number of peaks in the standard.
276
For use with GeneScan
™ ™
GeneScan 600 LIZ Size Standard v2.0 60–600 bp with normalization,
™ ™
GeneScan 600 LIZ Size Standard v2.0 20–600 bp with size standard
Actions4Library4Size standard.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
™
size standard
for applications that have primer
peaks that obscure the 20-mer
and 40-mer peaks of the GS600
size standard
normalization
Analysis range