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Appendix B Run modules and dye sets
B
Dye sets
Table 18 Fragment analysis run modules (continued)
Run
Run module
module
name
type
Long
LongFragAnalysi
fragment
s50_POP7
analysis
LongFragAnalysi
s50_POP7xl
SNaPshot
SNaPshot50_PO
™
P7
SNaPshot50_PO
P7xl
[1]
Throughput (samples/day): The total number of samples run in 23 hours (0.5 hour is required for user interaction and 0.5 hour is required for
warm-up time).
[2]
Resolution range: The range of bases over which the resolution (peak spacing interval divided by the peak width at half-maximum in a
™
™
GeneScan
600 LIZ
Size Standard or GeneScan
resolution range in ≥90% of samples.
[3]
Sizing precision: Standard deviation of sizes for one allele in the DS-33 install standard sized with the GS600 LIZ size standard across multiple
capillaries in the same run. For one injection to pass, 100% of the alleles in that injection must meet the intra-run sizing precision specifications.
The table shows the sizing precision of 100% of alleles in ≥90% of samples.
[4]
Throughput is an estimate extrapolated from average run time.
[5]
Not applicable because of the size of the fragments collected in the run.
Dye sets
Table 19 Sequence analysis dye sets
™
E (v1.1 BigDye
™
Z (v3.1 BigDye
Table 20 Fragment analysis dye sets
E5
G5
J6 or J6-T
F
D
530
Configuration
Cap.
length
Pol. type
(cm)
™
50
POP-7
50
POP-7
™
™
1200 LIZ
Dye set
Terminator)
Terminator)
Dye set
SeqStudio
[1]
23 hours throughput
Run
(8-
(24-
time
cap.)
cap.)
(min)
≤125
≥88
≥264
≤30
≥368
≥1,104
™
Size Standard sample sized with a third order fit) is ≥1. The table shows the
Rapid DNA sequencing
DNA sequencing
™
SNaPshot
kit
DNA sizing for 5-dye chemistry
DNA sizing for 6-dye chemistry
DNA sizing for 4-dye chemistry
DNA sizing for 4-dye chemistry
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Performance
Sizing precision
[2]
Range
50–
401–
400bp
600bp
≥40 to
<0.15
<0.30
≤700
≥40 to
<0.50
NA
≤120
Application
Application
[3]
601–
1,200
bp
<0.45
[5]
NA
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