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Chapter 6 Start and monitor a run
6
Auto-spectral calibration during an injection
Tap an alert to display the Notifications screen. The number above the
1
notifications for the plate.
Examples of plate alerts are displayed below.
2. Tap OK to return to the home screen.
Auto-spectral calibration during an injection
Auto-spectral calibration overview
During data collection, the instrument detects the signal of fluorescent dyes that are attached to DNA
amplicons. Although each dye emits its maximum fluorescence at a different wavelength, there is
some overlap in the emission spectra between the dyes. A spectral calibration creates a reference
deconvolution matrix (also referred to as a dye matrix) that corrects for the overlapping fluorescence
emission spectra of the dyes. A deconvolution matrix is created for each capillary in a capillary array.
A dye matrix is created when you run the manual spectral calibration function. The dye matrix is then
optimized and updated by the auto-spectral calibration function, which is performed automatically for
each capillary during each injection.
In the auto-spectral calibration process, the instrument uses spectral data from the samples to optimize
and update the dye matrix from the manual calibration, which generates an auto-spectral dye matrix.
This feature optimally reduces pull‑up (false secondary peaks under a true peak) that can be caused by
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SeqStudio
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Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
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Troubleshooting
