Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 350

Chapter 12 Run calibrations and install checks
12
Perform an install run
The red line in the plot is the sizing curve. The install run uses the Global Southern sizing method.
You can tap View by to filter results by Pass (
For more information, see "Use the electropherogram screen"
Pass/fail criteria and results for the fragment install check
The software evaluates peaks in the data for each capillary. To be identified as a possible allele, peaks
must be within the following ranges. The nominal allele size, or reference bin size, is hard-coded in the
software.
# Allele peaks
# Size standard peaks
Sizing precision (standard deviation of the observed allele fragment
sizes for all capillaries)
For all peaks that meet the acceptance criteria and are within the nominal size range, the software
calculates the Average Peak Height and the Sizing Precision. Peaks that meet the following
thresholds pass.
The following information is included in an exported install report.
350
) or Flagged (
Result
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
or ).
on page 173.
Acceptance criteria
15 per capillary
34 per capillary
<0.15 base pairs (bp)