Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 300

Chapter 11 Manage library resources on the instrument
11
Analysis settings library
Description
Slope thresholds peak
start
Slope thresholds peak end
Smoothing
Use baselining
Minimum peak half width
300
• Peak start—The peak starts when the first derivative (slope of the tangent) in
the beginning of the peak signal before the inflection point becomes equal to or
exceeds this setting. This threshold is set to 0 by default, which means that the
peak will normally start at the leftmost point where the slope of the tangent is
closest to 0° (horizontal line). A value other than 0 moves the peak start point
toward its center. The value entered must be non-negative.
• Peak end—The peak ends when the first derivative (slope of the tangent) in the
end of the peak signal after the inflection point becomes equal to or exceeds
this setting. This value is set to 0 by default, which means that the peak will
normally end at the rightmost point where the slope of the tangent is closest to 0°
(horizontal line). A value other than 0 moves the peak end point toward its center.
The value entered in this field must be non-positive.
Select an option to smooth the outline of peaks and reduce the number of false peaks
detected:
• None (default)—Use for data with sharp, narrow peaks of interest.
• Light—Use for typical data. Light smoothing slightly reduces peak height.
• Heavy—Use for data with very sharp, narrow peaks of interest. Heavy smoothing
can significantly reduce peak height.
Note: Smoothing is applied before peaks are detected.
Specify a window to adjust the baseline signals of all detected dye colors to the same
level for an improved comparison of relative signal intensity. Note the following:
• A small baseline window relative to the width of a cluster, or grouping of peaks
spatially close to each other, can result in shorter peak heights.
• Larger baseline windows relative to the peaks being detected can create an
elevated baseline, resulting in peaks that are elevated or not resolved to the
baseline.
Specify the number of scans across which the minimum full peak width at half
maximum required for peak detection.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Setting