Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 307

3. Tap View details to view the results for each capillary.
Average peak height
Uniformity (peak height
similarity)
Spacing (capillary spacing)
Intensity (individual peak
height)
Signal optimization factor
Fragment analysis on 24-capillary instruments only: A signal optimization factor is calculated
for each capillary during spatial calibration. The factor for each capillary is applied during data
collection to minimize optical variation effects and to increase signal uniformity between capillaries.
Note: The signal optimization factor is calculated using a fitted curve method. The fitted curve
method minimizes background and reduces noise. The adjusted spatial intensity, not the spatial
intensity displayed for the capillary, is used to calculate the signal optimization factor.
4. Tap Done.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Attribute
Chapter 12 Run calibrations and install checks
Calculation
(sum of all peak heights)
divided by
(number of peaks)
(standard deviation)
divided by
(average peak height)
Maximum spacing – minimum
spacing
Peak height
Described below
Run a spatial calibration
Threshold
• 8-cap: ≥6,400 RFU
• 24-cap: ≥2,500 RFU
0.2
2 pixels
1,000 RFU
—
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