Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 274

Chapter 11 Manage library resources on the instrument
11
Run modules library
(continued)
Setting
Do not change the following settings unless advised to do so by support person.
Voltage tolerance (volts)
Voltage step interval (sec)
Voltage # of steps
First read-out time (ms)
Fragment analysis only: Normalization parameters—Leave at default settings (for information on how these
parameters are used, see "Size standard normalization feature"
Norm. target
Norm. factor threshold min
Norm. factor threshold
max
Normalization factor
calculated by the software
(this value is not displayed
in the software)
274
Maximum allowed voltage variation.
Dwell time at each voltage ramp step.
Number of voltage ramp steps to reach run voltage.
The interval of time for a data point to be produced.
Normalization target. The expected average RFU for the subset of peaks in the
™ ™
GeneScan 600 LIZ Size Standard v2.0 used for normalization.
The default value for each run module has been experimentally determined based on
the average peak height of selected peaks in the GS600 size standard with a specific
injection time.
Optimize the normalization target for your size standard peak heights under your run
conditions.
Normalization factor thresholds. The passing range for the normalization factor (the
default range is 0.3–3.0).
IMPORTANT! Increasing the factor threshold above 3.0 may cause amplification of
noise.
If the calculated normalization factor is outside the normalization factor range, the
software multiplies the peak heights of the sample by the min or max normalization
factor threshold setting. For example, if the normalization factor range is 0.3–3.0 and
the calculated normalization factor is 5, the software applies a normalization factor of
3.0.
Average peak height of the subset of peaks in the GeneScan
v2.0 used for normalization divided by the normalization target.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Description
on page 278).
™ ™
600 LIZ Size Standard