Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 380

Chapter 13 Maintain the Instrument
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Maintain the pump (wizards)
3. Attach the syringe to the forward-facing Luer fitting at the top of the pump block. Hold the fitting
with one hand while threading the syringe onto the fitting with the other hand.
Luer fitting
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Water trap waste
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4. Grasp the attached syringe and turn counterclockwise approximately one-half turn.
5. Slowly depress the plunger.
IMPORTANT! DO NOT USE EXCESSIVE FORCE when you push the syringe plunger which can
damage the water trap seals. Take approximately 30 seconds to flush 5 mL of distilled or deionized
water through the trap.
Note: Because the water trap volume is approximately 325 μL, a relatively small volume of water is
adequate for complete flushing. However, a larger volume improves flushing as long as force and
flow rate are kept within the limits given above.
6. Remove the syringe from the Luer fitting. Hold the fitting with one hand while turning the syringe
counterclockwise with the other hand.
7. Close the Luer fitting by lightly turning clockwise until the fitting seals snugly against the block. Do
not overtighten.
8. Empty the water trap waste container, then reinstall it.
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SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide