Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual page 351

Genetic analyzer with instrument software v1.0
Table of Contents

Advertisement

Result
Min Peak Height
Sizing precision
Pass/Fail
For information only
Nominal Size
Mean size
Mean peak height
Peak Height % >
Min
Sizing Precision
Sizing Accuracy
Export fragment analysis install run logs or report
1. In the plot screen or the results per capillary screen, tap Actions.
2. Tap the item to export:
• Logs (ZIP)—Contains the install run report (PDF), data files (FSA), peak tables (CSV), install run
file (CID), and log files.
Log file name format: InstrumentName_Fragment Analysis_RunDate_Timestamp.zip,
for example: Instrument1_Fragment Analysis_20201021_121952.zip
• Report (PDF)— Contains the results of the install run.
Report file name format: InstrumentName_Fragment
Analysis_RunDate_Timestamp.pdf, for example: Instrument1_Fragment
Analysis_20201021_121952.pdf
3. Select the export location, then tap Next.
Note: It can take up to 15 seconds for the instrument to recognize a USB drive after the drive is
inserted in a USB port. exFAT-formatted USB drives are recommended.
For information on connecting to a network drive, see "Connect the software to a network drive
(software settings)"
4. When the export is complete, click Close to close the message box.
SeqStudio
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Description
Minimum of peak heights for observed
allele peaks of the included capillaries.
Standard deviation of the observed allele
fragment sizes
Alleles with a sizing precision and minimum peak height that do not meet thresholds
fail.
Expected allele fragment peak size (bp).
Average fragment size for the observed allele peaks.
Average peak height for the observed allele peaks.
Percentage of observed allele peaks with a peak height above the minimum
threshold.
Standard deviation of the observed allele fragment sizes vs the expected fragment
sizes.
Difference between the expected allele size and the mean allele size.
on page
443.
Chapter 12 Run calibrations and install checks
Threshold
>175 RFU
<0.15 for expected alleles
12
Perform an install run
351

Advertisement

Table of Contents
loading

This manual is also suitable for:

A50369

Table of Contents