Sequence Analysis Results; Parts Of The Sequencing Electropherogram Screen - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Sequence analysis results

Parts of the sequencing electropherogram screen

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Quality Value bars. For more information, see "Understanding Quality Values (QVs)"
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Pure basecall with QV ≥20
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Pure basecall with QV 15–19
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Pure basecall with QV <15
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Mixed basecall
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Basecalls—The background of a mixed basecall is flagged in red. You can set the secondary peak threshold, as
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a percentage of the primary peak, for consideration as a mixed basecall by the basecalling algorithm. Reaching
this threshold is a necessary but not sufficient condition for arriving at a mixed base determination. See "Analysis
settings—Sequencing"
Trace color hide/show—Tap to open, then tap a color to hide or show.
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Analyzed trace.
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Thumbnail trace—Drag the center of the pane in the thumbnail trace to display another trace area in the top pane.
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Drag the right or left handle of the pane to zoom horizontally.
Sample name and result summary. See "Sequencing results and well details"
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Trace tab—Tap to view the raw, EPT, or analyzed trace for the well. If the raw trace is blank, press-drag the thumbnail
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pane over the region of data to view.
Zoom tools—Tap to open.
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Well details—Tap to display well details. See "Sequencing results and well details"
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SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
on page 291.
Chapter 7 View results on the instrument

Sequence analysis results

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on page 178.
on page 176.
on page 176.
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