Perform A Spatial Calibration - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Genetic analyzer with instrument software v1.0
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Perform a spatial calibration

A spatial calibration is automatically performed when you use maintenance wizards to install a capillary
array (described in "Maintenance wizard overview"
This section describes how to manually perform a spatial calibration if you interact with the capillary
array without using a wizard (for example, if you move the instrument or open the detection cell heater
door). You can alternatively run the spatial calibration maintenance wizard (see "Maintenance wizard
overview"
on page
1. From the home screen, tap
2. Use the default Fill setting to fill the capillary array with polymer before starting the calibration.
Filling the capillary array ensures that no residual fragments are present in the capillary array that
can interfere with the spatial calibration and signal optimization.
Note: If you have previously run a spatial calibration and know that the capillary array is filled with
fresh polymer, you can select No Fill.
3. Tap Recalibrate.
Note: If a spatial calibration has not previously been performed for the installed capillary array, the
button displays Start calibration.
When the spatial calibration is complete, the Spatial Calibration screen is displayed.
SeqStudio
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
359).
Actions4Maintenance4Calibration4Spatial calibration.
Chapter 12 Run calibrations and install checks
on page
359).
12
Run a spatial calibration
305

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