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Chapter 13 Maintain the Instrument
13
Replenish, change, flush, and store polymer (wizards)
Replenish, change, flush, and store polymer (wizards)
IMPORTANT!
·
Wear appropriate protection, including gloves, laboratory goggles, and coat whenever you work with
the fluids used on this instrument, or parts that may come into contact with these fluids.
·
Use only the parts listed in Appendix D, "Catalog numbers".
·
To minimize background fluorescence, use clean, powder-free gloves whenever you handle the pump
assembly or any item in the polymer path.
Precautions for use
• Do not reuse a polymer pouch that has been installed on another type of instrument. For example,
if you remove a partially used polymer pouch from an 8-capillary instrument, do not install that
polymer pouch on a 24-capillary instrument.
• If you remove a polymer pouch for storage (2–8°C), place a pouch cap (Cat. No. 4462785) onto
the pouch. Install an empty pouch or conditioning reagent pouch on the instrument to prevent
desiccation of any residual polymer on the connector. Follow the instructions in the maintenance
wizards to ensure proper installation of the polymer pouch.
Replenish polymer or change polymer type (wizard)
For the time required, see "Maintenance wizard overview"
IMPORTANT! During the Change polymer type wizard, you can optionally replace the buffers and/or
the capillary array. If the CBC is not in the forward, home position when you need to install or remove
the CBC or capillary array, see "Check the position of the cathode buffer container (CBC)"
1. Allow the refrigerated polymer to equilibrate to room temperature (15–30°C) before use.
2. In the home screen, tap
type or Replenish polymer.
3. Follow the wizard instructions. See "Replenish Polymer maintenance wizard"
"Change Polymer Type (with change array option) maintenance wizard"
instructed to install the polymer, refer to this procedure.
4. Check the expiration date on the label to ensure that the polymer is not expired and will not expire
during intended use.
IMPORTANT! Do not use if the product is expired, if the pouch or label is damaged, or if the top
seal is missing or damaged.
5. When instructed to install the polymer, peel off the seal at the top of the pouch fitting.
Ensure that you completely remove the seal. If remnants of the seal are attached to the pouch
inlet when you install the polymer, the remnants can be pushed into the pouch and clog the pouch
fitting.
370
Actions4Maintenance4Maintenance wizards4Change polymer
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
on page
359.
on page
on page
483.
on page 417
or
394. When
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