Sequencing Results; Fragment Analysis Results; Cancel Injections Or Specify Re-Injections In The Remote Monitoring Software - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Chapter 8 Use the Remote Monitoring software to monitor runs, view results, and use cloud analysis
8
Monitor a run from the Remote Monitoring software

Sequencing results

Result
QC (Sample QC)
CRL (Contiguous Read
Length)

Fragment analysis results

Setting
QC (Sample QC)
Sizing quality (SQ)

Cancel injections or specify re-injections in the Remote Monitoring software

IMPORTANT! You can cancel an injection in the Remote Monitoring software, but you cannot resume
the run. You can resume the run from the instrument only.
IMPORTANT! To cancel injections, re-inject, or pause the instrument, you must be signed in to the
instrument and to Thermo Fisher
If another user is signed in to the instrument when you select any of these commands, a message is
displayed in the Remote Monitoring software indicating that the request was not sent to the instrument.
1. In the Plates tab, select an injection in the Injection list pane.
2. In the Sample QC field, click
Note: If more than one injection is selected, nothing is displayed when you click
3. Select the Re-inject or Cancel injection options.
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• —All QC tests passed.
•
—At least 1 warning quality alert was triggered.
•
—At least 1 failing quality alert was triggered.
For more information see "Sample QC and quality alerts"
The longest uninterrupted segment of basecalls with an average Quality Value (QV)
≥20.
In addition to evaluating the QV of a basecall, the software considers the QV of
adjacent basecalls within a 21-bp moving window to determine a contiguous read
length based on quality values: the software starts from the 5' end and calculates the
average QV across a moving window size of 21, sliding 1 bp at a time, to the 3' end.
The resulting longest contiguous segment is determined as the CRL.
•
—All QC tests passed.
•
—At least 1 warning quality alert was triggered.
•
—At least 1 failing quality alert was triggered.
For more information see "Sample QC and quality alerts"
For more information see "Sample QC and quality alerts"
™
Connect with the same cloud profile.
(Actions).
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Description
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Description
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(Actions).