Prepare For Custom Dye Set Spectral Calibration; Prepare For Spectral Calibration - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Chapter 12 Run calibrations and install checks
12
Run a spectral calibration

Prepare for custom dye set spectral calibration

Before you begin, create a custom dye set in the dye set library. See "Create a custom dye set"
page 286.

Prepare for spectral calibration

Prepare the instrument
1. Display the consumables dashboard by tapping
screen (if the consumables dashboard is not displayed, tap
2. Check consumables status (see "Check consumables status"
• Consumables are not expired
• Adequate injections remain for consumables
IMPORTANT! For install runs, use polymer that has been on the instrument ≤3 days. Slight peak
shifts can occur with polymer that has been on the instrument >3 days.
3. Ensure that buffer levels are at the fill lines (see "Check buffer fill levels"
4. Check the oven and detection cell temperature to ensure they are at the correct temperature (see
"Preheat the instrument oven and the detection cell"
5. Check the pump assembly for bubbles and run the Remove Bubble wizard if needed (see
"Remove bubbles from the polymer pump (wizard)"
Prepare the spectral calibration standard
For the instructions to prepare the standard, see the product information sheet. To find the product
information sheet, go to www.thermofisher.com, search for the product, then scroll to the bottom of
the product page.
Prepare the matrix standard appropriate for your application as described in the product insert.
Note: If you use the BigDye
™
BigDye v3.1 matrix standards. For more information, see "Using BigDye
chemistry" on page
DS-02 Matrix Standard Kit (Dye Set E5)
DS-30 Matrix Standard Kit (Dye Set D)
DS-31 Matrix Standard Kit (Dye Set D with VIC
DS-32 Matrix Standard Kit (Dye Set F)
DS-33 Matrix Standard Kit (Dye Set G5)
DS-36 Matrix Standard Kit (Dye Set J6)
DS-37 Matrix Standard Kit (Dye set J6‑T, 6‑dye)
314
™
Direct Cycle Sequencing Kit, perform the spectral calibration with
312.
Name
™ dye)
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
(Dashboard) at the top right of the home
or ).
on page 66). Ensure that:
on page
on page 68).
on page 377).
™
Direct Cycle Sequencing Kit
on
70).
Cat. No.
4323014
4345827
4345829
4345831
4345833
4425042
A31234