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Spectral calibration troubleshooting
See also "QC errors—Spectral calibration (manual)"
Observation
"Calibration from manual"
is reported for spectral
calibration status, but no pull-
up peaks are observed
"Calibration from manual"
is reported for spectral
calibration status and pull-up
peaks are observed
"Calibration from sample"
is reported for spectral
calibration status
Spatial and spectral
calibration reports list original
date of capillary array
installation (not the date of
re-installation)
Spectral calibration failed
SeqStudio
™
Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
Possible cause
Auto-spectral calibration
was not used. For
more information, see "Auto-
spectral calibration during an
injection"
on page
150.
Auto-spectral calibration was
not used because the sample
possibly contains overlapping
peaks that are labelled
with different dyes. For
more information, see "Auto-
spectral calibration during an
injection"
on page
150.
IMPORTANT!
Spectral
calibration status is reported
for informational and
tracking purposes only. This
calibration status does not
reflect the quality of the
sample or affect the results.
However, use of the manual
spectral calibration instead of
the auto-spectral calibration
can increase the likelihood of
pull-up peaks.
Auto-spectral calibration is
run on all samples and
generates the status.
See "Auto-spectral calibration
during an injection"
on
page
150.
—
The dye set definition
that is selected for the
spectral calibration does not
match the spectral calibration
standard used for the run.
Appendix A Troubleshooting
Spectral calibration troubleshooting
on page
492.
Recommended action
No action. Normal occurrence.
Perform a manual spectral calibration, then repeat
the run. See "Run a spectral calibration"
page
310.
IMPORTANT!
When running samples that
contain overlapping peaks that are labelled with
different dyes, manual spectral calibration is
required after changing the capillary array or
moving the instrument.
No action. Normal occurrence.
No action. Normal occurrence.
Specify the correct dye set for the spectral
calibration.
A
on
523
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Table of Contents
Troubleshooting
