Fill Array With Polymer Maintenance Wizard; Remove Bubbles Maintenance Wizard - Thermo Scientific appliedbiosystems SeqStudio Flex Series User Manual

Change Polymer Type—Flush array and spatial calibration
The final step in the wizard is to flush the capillary array with polymer and perform a spatial calibration.
A spatial calibration associates the signal from each capillary to a specific position on the CCD camera.
Tap Flush array and spatial calibration.
Wait ~15 minutes for the flush array and spatial calibration procedures to complete.

Fill Array with Polymer maintenance wizard

Fill Array with Polymer
Use the Fill Array with Polymer maintenance wizard to fill the capillary array with polymer. This wizard
can be useful for troubleshooting.
Note: The capillary array is automatically filled with polymer during each injection and when running
other maintenance wizards that affect the pump.
Item
Time required
Materials required
Tap Next to continue.

Remove Bubbles maintenance wizard

Remove Bubbles—Time and materials required
Use the Remove Bubbles maintenance wizard to remove bubbles from the fluid pathway. This function
is automatically performed during other wizards as needed.
Run this wizard if you observe bubbles in the fluidic path.
IMPORTANT! Running this wizard consumes polymer. Do not run this wizard excessively.
Note: A polymer pouch includes a small reserve volume that is used for the Remove Bubbles
maintenance wizard, which consumes ~350 μL of polymer. The reserve volume is sufficient to run the
wizard ~4 times (including the remove bubbles step during other maintenance wizards). If you manually
run the Remove Bubbles maintenance wizard >4 times, the volume of polymer that is available for
samples may be depleted.
Item
Time required
Materials required
Tap Next to continue.
SeqStudio ™ Flex Series Genetic Analyzer with Instrument Software v1.0 User Guide
~5 minutes
None
~7 minutes
None
Chapter 13 Maintain the Instrument
Maintenance wizard procedures
Requirements
Requirements
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